{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T19:09:59Z","timestamp":1772824199012,"version":"3.50.1"},"reference-count":49,"publisher":"BMJ","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"published-print":{"date-parts":[[1995,6]]},"abstract":"<jats:p>p53 Protein accumulation in early gastric carcinoma was studied in relation to the histological type (Lauren classification) and the type of growth pattern, including the chronology of p53 protein accumulation during carcinogenesis. Forty five, paraffin embedded gastrectomy specimens from early carcinomas were examined for the presence of chronic atrophic gastritis, subtypes of intestinal metaplasia, and dysplasia. The Lauren type and the type of growth pattern were reassessed for all early carcinomas. p53 Protein accumulation was examined using the monoclonal antibody DO-7. Complete absence of p53 protein accumulation was observed in normal gastric mucosa, chronic atrophic gastritis, and intestinal metaplasia, irrespective of subtype. In gastric dysplasia (one mild, two moderate, and one severe), only severe dysplasia was p53 positive. Intestinal type (n = 20) and diffuse type early gastric carcinomas (n = 25) were p53 positive in 70% and 52% of cases, respectively. Both tumour types differed significantly in the percentage of p53 positive tumour cells per tumour (p &lt; 0.01) and in staining intensity (p &lt; 0.05). No significant difference in p53 protein accumulation was found between early carcinomas with different types of growth pattern. It is concluded that p53 protein accumulation--usually reflecting missense p53 gene mutation--seems to be a late event in gastric carcinogenesis. Moreover, it is suggested that missense p53 gene mutation occurs in a final pathway common to both intestinal and diffuse type of early gastric carcinoma. Finally, the types of growth pattern do not seem to differ in p53 protein accumulation.<\/jats:p>","DOI":"10.1136\/gut.36.6.848","type":"journal-article","created":{"date-parts":[[2007,9,18]],"date-time":"2007-09-18T17:41:31Z","timestamp":1190137291000},"page":"848-852","source":"Crossref","is-referenced-by-count":44,"title":["Chronology of p53 protein accumulation in gastric carcinogenesis."],"prefix":"10.1136","volume":"36","author":[{"given":"M E","family":"Craanen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P","family":"Blok","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W","family":"Dekker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G J","family":"Offerhaus","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G N","family":"Tytgat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1995,6,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1111\/apm.1965.64.1.31","article-title":"The two histological main types of gastric carcinoma: diffuse and so-called intestinal-type carcinoma","volume":"64","author":"Lauren, P.","year":"1965","unstructured":"Lauren P. The two histological main types of gastric carcinoma: diffuse and so-called intestinal-type carcinoma. Acta Pathologica et Microbiolica Scandinavica 1965; 64: 31-49.","journal-title":"Acta Pathologica et Microbiolica Scandinavica"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/0046-8177(90)90234-V","article-title":"Gastric adenocarcinoma: Prognostic significance of several pathologic parameters and histologic classification","volume":"21","author":"Davessar, K.; Pezzulo, J.C.; Kessimian, N.; Hale, J.H.; Jauregui, H.O.","year":"1990","unstructured":"Davessar K, Pezzulo JC, Kessimian N, Hale JH, Jauregui HO. Gastric adenocarcinoma: Prognostic significance of several pathologic parameters and histologic classification. Hum Pathol 1990; 21: 325-32.","journal-title":"Hum Pathol"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1002\/ijc.2910030614","article-title":"Histologic types of gastric carcinoma in highand low-risk areas","volume":"3","author":"Munoz, N.; Correa, P.; Cuello, C.; Duque, E.","year":"1968","unstructured":"Munoz N, Correa P, Cuello C, Duque E. Histologic types of gastric carcinoma in highand low-risk areas. Int Jf Cancer 1968; 3: 809-18.","journal-title":"Int Jf Cancer"},{"key":"ref_4","first-page":"2554","article-title":"Precursors of gastric and esophageal cancer","volume":"50","author":"Correa, P.","year":"1982","unstructured":"Correa P. Precursors of gastric and esophageal cancer. Cancer 1982; 50: 2554-65.","journal-title":"Cancer"},{"key":"ref_5","first-page":"13","article-title":"A human model of gastric carcinogenesis","volume":"48","author":"Correa, P.","year":"1988","unstructured":"Correa P. A human model of gastric carcinogenesis. Cancer Res 1988; 48: 13 19-26.","journal-title":"Cancer Res"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1136\/jcp.45.4.319","article-title":"Helicobacter pylori infection and chronic gastritis in gastric cancer","volume":"45","author":"Sipponen, P.; Kosunen, T.U.; Valle, J.; Riihela, M.; Seppala, K.","year":"1992","unstructured":"Sipponen P, Kosunen TU, Valle J, Riihela M, Seppala K. Helicobacter pylori infection and chronic gastritis in gastric cancer. J Clin Pathol 1992; 45: 319-23.","journal-title":"J Clin Pathol"},{"key":"ref_7","first-page":"1370","article-title":"Helicobacter pyloni and early gastric cancer","volume":"35","author":"Craanen, M.E.; Blok, P.; Dekker, W.; Tytgat, G.N.J.","year":"1994","unstructured":"Craanen ME, Blok P, Dekker W, Tytgat GNJ. Helicobacter pyloni and early gastric cancer. Gut 1994: 35: 1370-2.","journal-title":"Gut"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1056\/NEJM199110173251603","article-title":"Helicobacter pylori infection and the risk of gastric carcinoma","volume":"325","author":"Parsonnet, J.; Friedman, G.D.; Vandersteen, J.P.; Chang, Y.; Vogelman, J.H.; Orentreich, N.; Sibley, R.K.","year":"1991","unstructured":"Parsonnet J, Friedman GD, Vandersteen JP, Chang Y, Vogelman JH, Orentreich N, Sibley RK. Helicobacter pylori infection and the risk of gastric carcinoma. N EnglJ Med 1991; 325: 1127-31.","journal-title":"N EnglJ Med"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1132","DOI":"10.1056\/NEJM199110173251604","article-title":"Helicobacter pylori infection and gastric carcinoma among Japanese Americans","volume":"325","author":"Nomura, A.; Stemmermann, G.N.; Chyou, P.-H.; Kato, I.; Perez-Perez, G.I.; Blaser, M.J.","year":"1991","unstructured":"Nomura A, Stemmermann GN, Chyou P-H, Kato I, Perez-Perez GI, Blaser MJ. Helicobacter pylori infection and gastric carcinoma among Japanese Americans. N Engl Y Med 1991; 325: 1132-6.","journal-title":"N Engl Y Med"},{"key":"ref_10","first-page":"525","article-title":"Genetic alterations during colorectal tumor development","volume":"319","author":"Vogelstein, B.; Fearon, E.R.; Hamilton, SR, Kern; SE, Preisinger; AC, Leppert; M.","year":"1989","unstructured":"Vogelstein B, Fearon ER, Hamilton SR, Kern SE, Preisinger AC, Leppert M, et al. Genetic alterations during colorectal tumor development. NEnglJ7Med 1989; 319: 525-32."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"892","DOI":"10.1016\/0016-5085(92)90022-Q","article-title":"p53 Gene mutations in gastric and esophageal cancers","volume":"103","author":"Imazeki, F.; Omata, M.; Nose, H.; Ohto, M.; Isono, K.","year":"1992","unstructured":"Imazeki F, Omata M, Nose H, Ohto M, Isono K. p53 Gene mutations in gastric and esophageal cancers. Gastroenterology 1992; 103: 892-6.","journal-title":"Gastroenterology"},{"key":"ref_12","first-page":"3056","article-title":"Detection of frequent p53 gene mutations in primary gastric cancer by cell sorting and polymerase chain reaction single-strand conformation polymorphism analysis","volume":"51","author":"Tamura, G.; Kihana, T.; Nomura, K.; Terada, M.; Sugimura, T.; Hirohashi, S.","year":"1991","unstructured":"Tamura G, Kihana T, Nomura K, Terada M, Sugimura T, Hirohashi S. Detection of frequent p53 gene mutations in primary gastric cancer by cell sorting and polymerase chain reaction single-strand conformation polymorphism analysis. Cancer Res 1991; 51: 3056-8.","journal-title":"Cancer Res"},{"key":"ref_13","first-page":"5800","article-title":"p53 Gene mutations in gastric cancer metastases and in gastric cancer cell lines derived from metastases","volume":"51","author":"Yamada, Y.; Yoshida, T.; Hayashi, K.; Sekiya, T.; Yokota, J.; Hirohashi, S.","year":"1991","unstructured":"Yamada Y, Yoshida T, Hayashi K, Sekiya T, Yokota J, Hirohashi S, et al. p53 Gene mutations in gastric cancer metastases and in gastric cancer cell lines derived from metastases. 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Cancer 1983; 51: 320-6.","journal-title":"Cancer"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1002\/ijc.2910500604","article-title":"p53 Expression and prognosis in gastric carcinoma","volume":"50","author":"Martin, H.M.; Filipe, M.I.; Morris, R.W.; Lane, D.L.; Silvestre, F.","year":"1992","unstructured":"Martin HM, Filipe MI, Morris RW, Lane DL, Silvestre F. p53 Expression and prognosis in gastric carcinoma. Int J Cancer 1992; 50: 859-62.","journal-title":"Int J Cancer"},{"key":"ref_17","first-page":"589","article-title":"Gastric cancer with p53 overexpression has high potential for metastasising to lymph nodes","volume":"67","author":"Kakeji, Y.; Korenaga, D.; Tsujitani, S.; Baba, H.; Anai, H.; Maehara, Y.; Sugimachi, K.","year":"1993","unstructured":"Kakeji Y, Korenaga D, Tsujitani S, Baba H, Anai H, Maehara Y, Sugimachi K. Gastric cancer with p53 overexpression has high potential for metastasising to lymph nodes. BrJCancer 1993; 67: 589-93.","journal-title":"BrJCancer"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1038\/bjc.1992.314","article-title":"Prognostic significance of p53 overexpression in gastric and colorectal carcinoma","volume":"66","author":"Starzynska, T.; Bromley, M.; Ghosh, A.; Stern, P.L.","year":"1992","unstructured":"Starzynska T, Bromley M, Ghosh A, Stern PL. Prognostic significance of p53 overexpression in gastric and colorectal carcinoma. BrJ Cancer 1992; 66: 558-62.","journal-title":"BrJ Cancer"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1111\/j.1440-1746.1991.tb01467.x","article-title":"The Sydney system: A new classification of gastritis. Introduction","volume":"6","author":"Misiewicz, J.J.","year":"1991","unstructured":"Misiewicz JJ. The Sydney system: A new classification of gastritis. Introduction. J Gastroenterol Hepatol 1991; 6: 207-8.","journal-title":"J Gastroenterol Hepatol"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1111\/j.1440-1746.1991.tb01468.x","article-title":"The Sydney system: Histological division","volume":"6","author":"Price, A.B.","year":"1991","unstructured":"Price AB. The Sydney system: Histological division. J Gastroenterol Hepatol 1991; 6: 209-22.","journal-title":"J Gastroenterol Hepatol"},{"key":"ref_21","article-title":"Histochemistry of intestinal mucins","volume-title":"oesophageal pathology","author":"Filipe, M.I.","unstructured":"Filipe MI. Histochemistry of intestinal mucins. In: Whitehead R, ed. Gastrointestinal and oesophageal pathology. 1st ed. Edinburgh\/London\/Melbourne\/New York: Churchill Livingstone, 1989: 71.","edition":"1st ed"},{"key":"ref_22","first-page":"53","article-title":"WHO international histological classification of tumours. The histological typing of oesophageal and gastric tumors. Berlin\/Heidelberg\/New York\/Paris\/Tokyo","volume-title":"Pathomorphological diagnosis. Definition and gross classification of early gastric cancer","volume":"23","author":"Watanabe, H.; Jass, JR, Sobin; LH, eds","year":"1990","unstructured":"Watanabe H, Jass JR, Sobin LH, eds. WHO international histological classification of tumours. The histological typing of oesophageal and gastric tumors. Berlin\/Heidelberg\/New York\/Paris\/Tokyo: Springer Verlag, 1990 23 Murakami T. Pathomorphological diagnosis. Definition and gross classification of early gastric cancer. In: Murakami T, ed. Early gastric cancer. Gann monograph on cancer research. Vol 11. Tokyo: University of Tokyo Press, 1971: 53-5."},{"key":"ref_23","first-page":"577","article-title":"The use of the avidin-biotinperoxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody procedures","volume":"19","author":"Hsu, S.M.; Raine, L.; Fanger, H.","year":"1985","unstructured":"Hsu SM, Raine L, Fanger H. The use of the avidin-biotinperoxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody procedures. Jf Histochem Cytochem 1985; 19: 577-80.","journal-title":"Jf Histochem Cytochem"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1002\/path.1711660402","article-title":"p53 in tumour pathology: can we trust immunohistochemistry?","volume":"166","author":"Wynford-Thomas, D.","year":"1992","unstructured":"Wynford-Thomas D. p53 in tumour pathology: can we trust immunohistochemistry? J Pathol 1992; 166: 329-30.","journal-title":"J Pathol"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/path.1711720103","article-title":"p53 In tumour pathology: can we trust immunohistochemistry? - revisited","volume":"172","author":"Hall, P.A.; Lane, D.P.","year":"1994","unstructured":"Hall PA, Lane DP. p53 In tumour pathology: can we trust immunohistochemistry? - revisited. J Pathol 1994; 172: 1-4.","journal-title":"J Pathol"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1016\/0046-8177(94)90113-9","article-title":"p53 Immunohistochemistry: A word of caution","volume":"25","author":"Battifora, H.","year":"1994","unstructured":"Battifora H. p53 Immunohistochemistry: A word of caution. Hum Pathol 1994; 25: 435-6.","journal-title":"Hum Pathol"},{"key":"ref_27","first-page":"3651","article-title":"Irradiationinduced micronuclei from mouse hepatoma cells accumulate high levels of the tumor suppressor protein p53","volume":"54","author":"Unger, C.; Kress, S.; Buchmann, A.; Schwarz, M.","year":"1994","unstructured":"Unger C, Kress S, Buchmann A, Schwarz M. Irradiationinduced micronuclei from mouse hepatoma cells accumulate high levels of the tumor suppressor protein p53. Cancer Res 1994; 54: 3651-5.","journal-title":"Cancer Res"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1997","DOI":"10.1128\/MCB.14.3.1997","article-title":"Accumulation of p53 in a mutant cell line defective in the ubiquitin pathway","volume":"14","author":"Chowdary, D.R.; Dermody, J.J.; Jha, K.K.; Ozer, H.L.","year":"1994","unstructured":"Chowdary DR, Dermody JJ, Jha KK, Ozer HL. Accumulation of p53 in a mutant cell line defective in the ubiquitin pathway. Mol Cell Biol 1994; 14: 1997-2003.","journal-title":"Mol Cell Biol"},{"key":"ref_29","first-page":"5006","article-title":"Genetic basis for p53 overexpression","volume-title":"human breast cancer. Proc Nad Acad Sci USA","volume":"88","author":"Davidoff, A.M.; Humphrey, P.A.; Iglehart, J.D.; Marks, JR","year":"1991","unstructured":"Davidoff AM, Humphrey PA, Iglehart JD, Marks JR. Genetic basis for p53 overexpression in human breast cancer. Proc Nad Acad Sci USA 1991; 88: 5006-10."},{"key":"ref_30","first-page":"2979","article-title":"Overexpression and mutation of p53 in epithelial ovarian cancer","volume":"51","author":"Marks, JR, Davidoff; AM, Kerns; BJ, Humphrey; PA, Pence; JC, Dodge; R.K.","year":"1991","unstructured":"Marks JR, Davidoff AM, Kerns BJ, Humphrey PA, Pence JC, Dodge RK, et al. Overexpression and mutation of p53 in epithelial ovarian cancer. Cancer Res 1991; 51: 2979-84.","journal-title":"Cancer Res"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1016\/0140-6736(90)90801-B","article-title":"Increased expression of mutant forms of p53 oncogene in primary lung cancer","volume":"335","author":"Iggo, R.; Gatter, K.; Bartek, J.; Lane, D.; Harris, A.L.","year":"1990","unstructured":"Iggo R, Gatter K, Bartek J, Lane D, Harris AL. Increased expression of mutant forms of p53 oncogene in primary lung cancer. Lancet 1990; 335: 675-9.","journal-title":"Lancet"},{"key":"ref_32","first-page":"551","article-title":"Immunohistochemical evidence of p53 overexpression in gastric epithelial dysplasia","volume":"1","author":"Rugge, M.; Shiao, Y.H.; Correa, P.; Baffa, R.; Dimario, F.","year":"1992","unstructured":"Rugge M, Shiao YH, Correa P, Baffa R, Dimario F. Immunohistochemical evidence of p53 overexpression in gastric epithelial dysplasia. Cancer Epidemiology - Biomarkers and Prevention 1992; 1: 551-4.","journal-title":"Cancer Epidemiology - Biomarkers and Prevention"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1002\/path.1711700310","article-title":"Expression of p53 protein in normal, dysplastic, and malignant gastric mucosa: an immunohistochemical study","volume":"170","author":"Joypaul, B.V.; Newman, E.L.; Hopwood, D.; Grant, A.; Qureshi, S.; Lane, D.P.; Cuschieri, A.","year":"1993","unstructured":"Joypaul BV, Newman EL, Hopwood D, Grant A, Qureshi S, Lane DP, Cuschieri A. Expression of p53 protein in normal, dysplastic, and malignant gastric mucosa: an immunohistochemical study. J Pathol 1993; 170: 279-83.","journal-title":"J Pathol"},{"key":"ref_34","first-page":"1916","article-title":"p53 Expression in precancerous gastric lesions - an immunohistochemical study of Pab 1801 monoclonal antibody on adenomatous and hyperplastic gastric polyps","volume":"88","author":"Lauwers, G.Y.; Wahl, S.J.; Melamed, J.; Rojascorona, R.R.","year":"1993","unstructured":"Lauwers GY, Wahl SJ, Melamed J, Rojascorona RR. p53 Expression in precancerous gastric lesions - an immunohistochemical study of Pab 1801 monoclonal antibody on adenomatous and hyperplastic gastric polyps. Am J Gastroenterol 1993; 88: 1916-9.","journal-title":"Am J Gastroenterol"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1111\/j.1365-2559.1980.tb02921.x","article-title":"Sulphomucins and precancerous lesions of the human stomach","volume":"4","author":"Jass, JR, Filipe; M.I.","year":"1980","unstructured":"Jass JR, Filipe MI. Sulphomucins and precancerous lesions of the human stomach. Histopathology 1980; 4: 271-9.","journal-title":"Histopathology"},{"key":"ref_36","first-page":"1110","article-title":"Detection of an increased incidence of early gastric cancer in patients with intestinal metaplasia type III who are closely followed up","volume-title":"Gut","volume":"32","author":"Rokkas, T.; Filipe, M.I.; Sladen, G.E.","year":"1991","unstructured":"Rokkas T, Filipe MI, Sladen GE. Detection of an increased incidence of early gastric cancer in patients with intestinal metaplasia type III who are closely followed up. Gut 1991; 32: 1110-3."},{"key":"ref_37","first-page":"935","article-title":"Early gastric cancer and intestinal metaplasia","volume":"3","author":"Craanen, M.E.; Blok, P.; Dekker, W.; Ferwerda, J.; Tytgat, G.N.J.","year":"1991","unstructured":"Craanen ME, Blok P, Dekker W, Ferwerda J, Tytgat GNJ. Early gastric cancer and intestinal metaplasia. Eur Jf Gastroenterol Hepatol 1991; 3: 935-40.","journal-title":"Eur Jf Gastroenterol Hepatol"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1637","DOI":"10.1016\/0016-5085(93)91058-P","article-title":"p53 Protein accumulation in Barrett's metaplasia, dysplasia and carcinoma: A follow-up study","volume":"105","author":"Younes, M.; Lebovitz, R.M.; Lechago, L.V.; Lechago, J.","year":"1993","unstructured":"Younes M, Lebovitz RM, Lechago LV, Lechago J. p53 Protein accumulation in Barrett's metaplasia, dysplasia and carcinoma: A follow-up study. Gastroenterology 1993; 105: 1637-42.","journal-title":"Gastroenterology"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1111\/j.1432-1033.1986.tb09919.x","article-title":"Isolation of human p53 specific monoclonal antibodies and their use in the studies of human p53 expression","volume":"159","author":"Banks, L.; Matlashewski, G.; Crawford, L.","year":"1986","unstructured":"Banks L, Matlashewski G, Crawford L. Isolation of human p53 specific monoclonal antibodies and their use in the studies of human p53 expression. Eur Jf Biochem 1986; 159: 529-34.","journal-title":"Eur Jf Biochem"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1242\/jcs.101.1.183","article-title":"Analysis of p53 expression in human tumours: an antibody raised against human p53 expressed in Escherichia coli","volume":"101","author":"Midgley, C.A.; Fisher, C.J.; Bartek, J.; Vojtesek, B.; Lane, D.; Barnes, D.M.","year":"1992","unstructured":"Midgley CA, Fisher CJ, Bartek J, Vojtesek B, Lane D, Barnes DM. Analysis of p53 expression in human tumours: an antibody raised against human p53 expressed in Escherichia coli. Jf Cel Sci 1992; 101: 183-9.","journal-title":"Jf Cel Sci"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/0022-1759(92)90122-A","article-title":"An immunohistochemical analysis of the human nuclear phosphoprotein p53","volume":"151","author":"Vojtesek, B.; Bartek, J.; Midgley, C.A.; Lane, D.P.","year":"1992","unstructured":"Vojtesek B, Bartek J, Midgley CA, Lane DP. An immunohistochemical analysis of the human nuclear phosphoprotein p53. Immunol Methods 1992; 151: 237-44. _","journal-title":"Immunol Methods"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1002\/path.1711720104","article-title":"An evaluation of six antibodies for immunohistochemistry of mutant p53 gene product in archival colorectal neoplasms","volume":"172","author":"Bass, I.O.; Mulder, J.W.R.; Offerhaus, G.J.A.; Vogelstein, B.; Hamilton, SR","year":"1994","unstructured":"Bass IO, Mulder JWR, Offerhaus GJA, Vogelstein B, Hamilton SR. An evaluation of six antibodies for immunohistochemistry of mutant p53 gene product in archival colorectal neoplasms. J Pathol 1994; 172: 5-12.","journal-title":"J Pathol"},{"key":"ref_43","first-page":"511","article-title":"p53 Alteration in gastric precancerous lesions","volume":"144","author":"Shiao, Y.-H.; Rugge, M.; Correa, P.; Lehmann, H.P.; Scheer, W.D.","year":"1994","unstructured":"Shiao Y-H, Rugge M, Correa P, Lehmann HP, Scheer WD. p53 Alteration in gastric precancerous lesions. Am J Pathol 1994; 144: 511-7.","journal-title":"Am J Pathol"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1111\/j.1365-2559.1993.tb01223.x","article-title":"Antigen retrieval by microwave irradiation lowers immunohistological detection thresholds","volume":"23","author":"McKee, P.H.; Hobbs, C.; Hall, P.A.","year":"1993","unstructured":"McKee PH, Hobbs C, Hall PA. Antigen retrieval by microwave irradiation lowers immunohistological detection thresholds. Histopathology 1993; 23: 377-9.","journal-title":"Histopathology"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1002\/path.1711720105","article-title":"Variations in immunohistochemical detection of p53 protein overexpression in cervical carcinomas with different antibodies and methods of detection","volume":"172","author":"Lambkin, H.A.; Mothersill, C.M.; Kelehan, P.","year":"1994","unstructured":"Lambkin HA, Mothersill CM, Kelehan P. Variations in immunohistochemical detection of p53 protein overexpression in cervical carcinomas with different antibodies and methods of detection. J Pathol 1994; 172: 13-8.","journal-title":"J Pathol"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1595","DOI":"10.1002\/j.1460-2075.1990.tb08279.x","article-title":"Activating mutations in p53 produce a common conformational effect. A monoclonal antibody specific for the mutant form","volume":"9","author":"Gannon, J.V.; Greaves, R.; Iggo, R.; Lane, D.P.","year":"1990","unstructured":"Gannon JV, Greaves R, Iggo R, Lane DP. Activating mutations in p53 produce a common conformational effect. A monoclonal antibody specific for the mutant form. EMBO J 1990; 9: 1595-602.","journal-title":"EMBO J"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1038\/362847a0","article-title":"p53 Is required for radiation induced apoptosis in mouse thymocytes","volume":"362","author":"Lowe, S.W.; Schmitt, E.M.; Smith, S.W.; Osborne, B.A.; Jacks, T.","year":"1993","unstructured":"Lowe SW, Schmitt EM, Smith SW, Osborne BA, Jacks T. p53 Is required for radiation induced apoptosis in mouse thymocytes. 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