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Lectin binding was detected by polyclonal antibodies using PAP staining to the surface and the parietal cell region of the stomach, the brush border epithelium of the small intestine and to the surface membrane of the caecum and colon. To characterise the metabolic changes in the gut organ weights, protein, RNA, DNA, and polyamine contents were measured. While phytohaemagglutinin induced a dose and time dependent growth of the small intestine by lengthening the tissue and thickening the gut wall by increasing the number of crypt cells, the lectin also changed the size and metabolism of the large intestine and pancreas, but this growth was by hypertrophy. Phytohaemagglutinin in the diet influences the size, metabolism, and function of the entire digestive tract. The lectin induced changes were fully or partially reversed within three days.<\/jats:p>","DOI":"10.1136\/gut.37.3.353","type":"journal-article","created":{"date-parts":[[2007,9,18]],"date-time":"2007-09-18T17:41:31Z","timestamp":1190137291000},"page":"353-360","source":"Crossref","is-referenced-by-count":75,"title":["Reversible effect of phytohaemagglutinin on the growth and metabolism of rat gastrointestinal tract."],"prefix":"10.1136","volume":"37","author":[{"given":"S","family":"Bardocz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G","family":"Grant","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S W","family":"Ewen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T J","family":"Duguid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D S","family":"Brown","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Englyst","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Pusztai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[1995,9,1]]},"reference":[{"key":"ref_1","first-page":"1283","article-title":"Phaseolus vulgaris lectin induces the growth and increases the polyamine content of rat small intestine in vivo","volume":"16","author":"Pusztai, A.; Grant, G.; Brown, D.S.; Ewen, S.W.B.; Bardocz, S.","year":"1988","unstructured":"Pusztai A, Grant G, Brown DS, Ewen SWB, Bardocz S. 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Kidney bean lectin-induced Escherichia coli overgrowth in the small intestine is blocked by GNA, a mannose-specific lectin. J Appl Bacteriol 1993; 75: 360-8.","journal-title":"J Appl Bacteriol"},{"key":"ref_9","first-page":"22","article-title":"Lectins and also bacteria modify the glycosylation of gut surface receptors in the rat","volume-title":"Glycoconj3","volume":"12","author":"Pusztai, A.; Ewen, S.W.B.; Grant, G.; Peumans, W.J.; Van Damme, E.J.M.; Coates, M.E.","year":"1995","unstructured":"Pusztai A, Ewen SWB, Grant G, Peumans WJ, Van Damme EJM, Coates ME, et al. Lectins and also bacteria modify the glycosylation of gut surface receptors in the rat. Glycoconj3 1995; 12: 22-35."},{"key":"ref_10","series-title":"Proceedings of the Interdisciplinary Conference on Effects ofFood on the Immune and Hormonal Systems. Zurich: Swiss Federal Institute of Technology and University of","first-page":"22","article-title":"Immune and hormonal effects of dietary lectins. 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