{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T08:45:54Z","timestamp":1784191554315,"version":"3.55.0"},"reference-count":32,"publisher":"BMJ","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"published-print":{"date-parts":[[1996,3]]},"abstract":"<jats:p>In healthy controls (n = 8) living in shigella endemic areas, accumulation of interferon gamma (IFN gamma) in the epithelial lining was seen in the rectal tissues. At the single cell level, however, few or no IFN gamma protein producing cells or mRNA expressing cells were detected at that site indicating the involvement of the whole large intestine in the production of IFN gamma in controls. Persistent numbers of IFN gamma producing cells were detected in the rectum of patients with Shigella dysenteriae type 1 infection (n = 8) throughout the course of disease with a tendency to increase in the convalescent stage. A significantly increased extra cellular deposition of secreted IFN gamma in tissue was seen in convalescence when compared with the acute stage (p &lt; 0.05). In addition, enzyme immunoassay showed increased stool concentration of IFN gamma in patients at the convalescent stage as well as in healthy controls. In situ hybridisation confirmed the results by showing increased frequency of IFN gamma mRNA containing cells at the late stage of the disease (p &lt; 0.05). Extensive message for IFN gamma was evident in cells in the lamina propria with no detectable transcripts in the surface epithelium. A colocalisation of IFN gamma with the IFN gamma receptor expression, predominantly found in the epithelial lining was detected by immunohistochemistry. Semiquantitative evaluation by computerised image analysis showed a gradual increased expression of IFN gamma and its corresponding receptor in the convalescent stage of shigellosis. This suggested progressive entrapment and binding of IFN gamma to its specific receptor at the local site. The enhanced surface expression of IFN gamma receptor evident at the convalescent stage of shigellosis was comparable to the constitutive level of expression in the healthy subjects. Thus, immunity to shigellosis correlated to up-regulation of IFN gamma production and expression of IFN gamma receptor.<\/jats:p>","DOI":"10.1136\/gut.38.3.328","type":"journal-article","created":{"date-parts":[[2007,9,18]],"date-time":"2007-09-18T17:41:31Z","timestamp":1190137291000},"page":"328-336","source":"Crossref","is-referenced-by-count":33,"title":["Local entrapment of interferon gamma in the recovery from Shigella dysenteriae type 1 infection."],"prefix":"10.1136","volume":"38","author":[{"given":"R","family":"Raqib","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Ljungdahl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A A","family":"Lindberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"U","family":"Andersson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Andersson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[1996,3,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4173","DOI":"10.1128\/IAI.59.11.4173-4179.1991","article-title":"Shiga toxin-associated haemolyticuraemic syndrome: combined cytotoxic effects of Shiga toxin, interleukin-1, B, and tumour necrosis factor alpha on human vascular endothelial cells in vitro","volume":"59","author":"Louise, C.B.; Obrig, T.G.","year":"1991","unstructured":"Louise CB, Obrig TG. Shiga toxin-associated haemolyticuraemic syndrome: combined cytotoxic effects of Shiga toxin, interleukin-1,B, and tumour necrosis factor alpha on human vascular endothelial cells in vitro. Infect Immun 1991; 59: 4173-9.","journal-title":"Infect Immun"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.1084\/jem.180.4.1307","article-title":"Acute inflammation causes epithelial invasion and mucosal destruction in experimental shigellosis","volume":"180","author":"Perdomo, O.J.J.; Cavaillon, J.M.; Huerre, M.; Ohayon, H.; Gounon, P.; Sansonette, P.J.","year":"1994","unstructured":"Perdomo OJJ, Cavaillon JM, Huerre M, Ohayon H, Gounon P, Sansonette PJ. Acute inflammation causes epithelial invasion and mucosal destruction in experimental shigellosis. J Exp Med 1994; 180: 1307-19.","journal-title":"J Exp Med"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1172\/JCI117015","article-title":"Polymorphonuclear leukocyte transmigration promotes invasion of colonic epithelial monolayer by Shigella flexneri","volume":"93","author":"Perdomo, O.J.J.; Gounon, P.; Sansonetti, P.J.","year":"1994","unstructured":"Perdomo OJJ, Gounon P, Sansonetti PJ. Polymorphonuclear leukocyte transmigration promotes invasion of colonic epithelial monolayer by Shigella flexneri. J Clin Invest 1994; 93: 633-43.","journal-title":"J Clin Invest"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"5085","DOI":"10.1128\/IAI.62.11.5085-5094.1994","article-title":"Purified Shiga-like toxins induce expression of pro inflammatory cytokines from murine peritoneal macrophages","volume":"62","author":"Tesh, V.L.; Ramegowda, B.; Samuel, J.E.","year":"1994","unstructured":"Tesh VL, Ramegowda B, Samuel JE. Purified Shiga-like toxins induce expression of pro inflammatory cytokines from murine peritoneal macrophages. Infect Immun 1994; 62: 5085-94.","journal-title":"Infect Immun"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1093\/infdis\/164.2.344","article-title":"Evaluation of the role of Shiga and Shiga-like toxins in mediating direct damage to human vascular endothelial cells","volume":"164","author":"Tesh, V.L.; Samuel, J.E.; Perera, L.P.; Sharefitn, J.B.; O'Brien, A.D.","year":"1991","unstructured":"Tesh VL, Samuel JE, Perera LP, Sharefitn JB, O'Brien AD. Evaluation of the role of Shiga and Shiga-like toxins in mediating direct damage to human vascular endothelial cells. JInfect Dis 1991; 164: 344-52.","journal-title":"JInfect Dis"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1328","DOI":"10.1172\/JCI117452","article-title":"Interleukin 1 is released by murine macrophages during apoptosis induced by Shigella flexneri","volume":"94","author":"C, Zychlinsky A.Fitting; J-M, Cavaillon; PJ, Sansonetti","year":"1994","unstructured":"Zychlinsky A. Fitting C, Cavaillon J-M, Sansonetti PJ. Interleukin 1 is released by murine macrophages during apoptosis induced by Shigella flexneri. J Clin Invest 1994; 94: 1328-32.","journal-title":"J Clin Invest"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1128\/IAI.63.1.289-296.1995","article-title":"Persistence of local cytokine production in shigellosis in acute and convalescent stages","volume":"63","author":"Raqib, R.; Lindberg, A.A.; Wretlind, B.; Bardhan, P.K.; Andersson, U.; Andersson, J.","year":"1995","unstructured":"Raqib R, Lindberg AA, Wretlind B, Bardhan PK, Andersson U, Andersson J. Persistence of local cytokine production in shigellosis in acute and convalescent stages. Infect Immun 1995; 63: 289-96.","journal-title":"Infect Immun"},{"key":"ref_8","first-page":"376","article-title":"Cytokine secretion in acute shigellosis is correlated to disease activity and detected more","volume":"171","author":"Raqib, R.; Wretlind, B.; Andersson, J.; Lindberg, A.A.","year":"1995","unstructured":"Raqib R, Wretlind B, Andersson J, Lindberg AA. Cytokine secretion in acute shigellosis is correlated to disease activity and detected more in stool than in plasma. J Infect Dis 1995; 171: 376-84.","journal-title":"in stool than in plasma. J Infect Dis"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1172\/JCI113938","article-title":"Interferon-y directly affects barrier function of cultured intestinal epithelial monolayers","volume":"83","author":"Madara, J.L.; Stafford, J.","year":"1989","unstructured":"Madara JL, Stafford J. Interferon-y directly affects barrier function of cultured intestinal epithelial monolayers. 7 Clin Invest 1989; 83: 724-7.","journal-title":"7 Clin Invest"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1083\/jcb.120.3.785","article-title":"Neutrophil migration across cultured intestinal epithelial monolayers is modulated by epithelial exposure to IFN--y in a highly polarised fashion","volume":"120","author":"Colgan, S.P.; Parkos, C.A.; Delp, C.; Arnaout, M.A.; Madara, J.L.","year":"1993","unstructured":"Colgan SP, Parkos CA, Delp C, Arnaout MA, Madara JL. Neutrophil migration across cultured intestinal epithelial monolayers is modulated by epithelial exposure to IFN--y in a highly polarised fashion. J Cell Biol 1993; 120: 785-98.","journal-title":"J Cell Biol"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1016\/0016-5085(94)90614-9","article-title":"Cytokines in intestinal inflammation: pathophysiological and clinical considerations","volume":"106","author":"Sartor, R.B.","year":"1994","unstructured":"Sartor RB. Cytokines in intestinal inflammation: pathophysiological and clinical considerations. Gastroenterology 1994; 106: 533-9.","journal-title":"Gastroenterology"},{"key":"ref_12","first-page":"591","article-title":"The role of TNF-a in T-cell-mediated inflammation depends on the Th1\/Th2 cytokine balance","volume":"82","author":"r, Hernandez-Pando; GAW, Rook","year":"1994","unstructured":"Hernandez-Pando r, Rook GAW. The role of TNF-a in T-cell-mediated inflammation depends on the Th1\/Th2 cytokine balance. Immunology 1994; 82: 591-5.","journal-title":"Immunology"},{"key":"ref_13","first-page":"2403","article-title":"Distribution of interferon-y receptor in human tissues","volume":"22","author":"Valente, G.; Ozmen, L.; Novelli, F.; Geuna, M.; Palestro, G.; Forni, G.","year":"1992","unstructured":"Valente G, Ozmen L, Novelli F, Geuna M, Palestro G, Forni G, et al. Distribution of interferon-y receptor in human tissues. EurJImmunol 1992; 22: 2403-12.","journal-title":"EurJImmunol"},{"key":"ref_14","first-page":"2407","article-title":"Release of reactive nitrogen intermediates and reactive oxygen intermediates from mouse peritoneal macrophages: comparison of activating cytokines and evidence for independent production.7 Immunol","volume":"141","author":"Ding, A.H.; Nathan, C.F.; Steuehr, D.J.","year":"1988","unstructured":"Ding AH, Nathan CF, Steuehr DJ. Release of reactive nitrogen intermediates and reactive oxygen intermediates from mouse peritoneal macrophages: comparison of activating cytokines and evidence for independent production.7 Immunol 1988; 141: 2407-12."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1002\/jlb.54.6.545","article-title":"Detection of intracellular interferon-g by light microscopy using an immunoperoxidase technique: correlation with the corresponding mRNA and protein product","volume":"54","author":"Dolhain, R.J.E.M.; Andersson, U.; Haar, N.T.; Brinkman, B.M.N.; Verweij, C.L.; Daha, M.R.","year":"1993","unstructured":"Dolhain RJEM, Andersson U, Haar NT, Brinkman BMN, Verweij CL, Daha MR, et al. Detection of intracellular interferon-g by light microscopy using an immunoperoxidase technique: correlation with the corresponding mRNA and protein product. Y Leukoc Biol 1993; 54: 545-51.","journal-title":"Y Leukoc Biol"},{"key":"ref_16","first-page":"69","article-title":"Bacterial toxin-induced cytokine production studied at the single-cell level Immunol","volume":"127","author":"Andersson, J.; Nagy, S.; Bjork, L.; Abrams, J.; Holm, S.; Andersson, U.","year":"1992","unstructured":"Andersson J, Nagy S, Bjork L, Abrams J, Holm S, Andersson U. Bacterial toxin-induced cytokine production studied at the single-cell level Immunol Rev 1992; 127: 69-96.","journal-title":"Rev"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1007\/BF00716936","article-title":"Sensitive mRNA detection using unfixed tissue: combined radioactive and non-radioactive in situ hybridisation histochemistry","volume":"98","author":"Dagerlind, A.; Friberg, K.; Bean, A.J.; Hokfelt, T.","year":"1992","unstructured":"Dagerlind A, Friberg K, Bean AJ, Hokfelt T. Sensitive mRNA detection using unfixed tissue: combined radioactive and non-radioactive in situ hybridisation histochemistry. Histochemistry 1992; 98: 39-49.","journal-title":"Histochemistry"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1038\/295503a0","article-title":"Expression of human immune interferon cDNA in E coli and monkey cells","volume":"295","author":"Gray, P.W.; Leung, D.W.; Pennica, D.; Yelverton, E.; Najarian, R.; Simonsen, C.C.","year":"1982","unstructured":"Gray PW, Leung DW, Pennica D, Yelverton E, Najarian R, Simonsen CC, et al. Expression of human immune interferon cDNA in E coli and monkey cells. Nature 1982; 295: 503-8.","journal-title":"Nature"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1038\/312724a0","article-title":"Human tumor necrosis factor: precursor structure, expression and homology to lymphotoxin","volume":"312","author":"Pennica, D.; Nedwin, G.E.; Hayflick, J.S.; Seeburg, P.H.; Derynck, R.; Palladino, M.A.; Kohr, W.J.","year":"1984","unstructured":"Pennica D, Nedwin GE, Hayflick JS, Seeburg PH, Derynck R, Palladino MA, Kohr WJ, et al. Human tumor necrosis factor: precursor structure, expression and homology to lymphotoxin. Nature 1984; 312: 724-9.","journal-title":"Nature"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"147","DOI":"10.4049\/jimmunol.139.1.147","article-title":"Internalization and degradation of receptor-bound interferon-y by murine macrophages. Demonstration of receptor recycling","volume":"139","author":"Celada, A.; Schreiber, R.D.","year":"1987","unstructured":"Celada A, Schreiber RD. Internalization and degradation of receptor-bound interferon-y by murine macrophages. Demonstration of receptor recycling. J Immunol 1987; 139: 147-53.","journal-title":"J Immunol"},{"key":"ref_21","first-page":"2675","article-title":"Localization of IFN-y receptor in first trimester placenta to trophoblasts but lack of stimulation of HLA-DRA, DRB, or invariant chain mRNA expression by IFN-","author":"Peyman, J.A.; Hammond, G.L.","unstructured":"Peyman JA, Hammond GL. Localization of IFN-y receptor in first trimester placenta to trophoblasts but lack of stimulation of HLA-DRA,-DRB, or invariant chain mRNA expression by IFN-.JImmunol 1994;-149: 2675-80."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1627","DOI":"10.1182\/blood.V77.8.1627.1627","article-title":"Interleukin-1 and interleukin-1 antagonism","volume":"77","author":"Dinarello, C.A.","year":"1991","unstructured":"Dinarello CA. Interleukin-1 and interleukin-1 antagonism. Blood 1991; 77: 1627-52.","journal-title":"Blood"},{"key":"ref_23","first-page":"721","article-title":"Inhibition of Shiga-like toxins by Brefeldin A. J7 Infect","volume":"171","author":"Donta, S.T.; Tomicic, T.K.; Donohue-Rolfe, A.","year":"1995","unstructured":"Donta ST, Tomicic TK, Donohue-Rolfe A. Inhibition of Shiga-like toxins by Brefeldin A. J7 Infect Dis 1995; 171: 721-4.","journal-title":"Dis"},{"key":"ref_24","first-page":"568","article-title":"Shiga toxin inhibition of endothelial cell IL-8, IL-6 and sICAM release is dependent on pre-stimulation with LPS, TNF-a or IL-1","volume":"6","author":"Kennedy, J.S.; Dinarello, C.A.","year":"1994","unstructured":"Kennedy JS, Dinarello CA. Shiga toxin inhibition of endothelial cell IL-8, IL-6 and sICAM release is dependent on pre-stimulation with LPS, TNF-a or IL-1, [Abstract]. Cytokine 1994; 6: 568."},{"key":"ref_25","first-page":"823","article-title":"Natural and recombinant interferons inhibit epithelial cell invasion by Shigella spp","volume":"52","author":"Niesel, D.W.; Hess, C.B.; Cho, Y.J.; Klimpel, K.D.; Klimpel, G.R.","year":"1986","unstructured":"Niesel DW, Hess CB, Cho YJ, Klimpel KD, Klimpel GR. Natural and recombinant interferons inhibit epithelial cell invasion by Shigella spp. Infect Immun 1986; 52: 823-33.","journal-title":"Infect Immun"},{"key":"ref_26","first-page":"1","article-title":"In vitro treatment of HEp-2 cells with human tumor necrosis factor-a and human interferons reduces invasiveness of Salmonella typhimurium","volume":"3","author":"Degre, M.; Bukholm, G.; Czarniecki, C.W.","year":"1989","unstructured":"Degre M, Bukholm G, Czarniecki CW. In vitro treatment of HEp-2 cells with human tumor necrosis factor-a and human interferons reduces invasiveness of Salmonella typhimurium. J Biol Regul Homeost Agents 1989; 3: 1-7.","journal-title":"J Biol Regul Homeost Agents"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1093\/clinids\/13.Supplement_4.S304","article-title":"Shiga toxin: intestinal cell receptors and pathophysiology of enterotoxic effects","volume":"13","author":"Keusch, G.T.; Jacewicz, M.; Mobassaleh, M.; Donhue-Rolfe, A.","year":"1991","unstructured":"Keusch GT, Jacewicz M, Mobassaleh M, Donhue-Rolfe A. Shiga toxin: intestinal cell receptors and pathophysiology of enterotoxic effects. Rev Infect Dis 1991; 13 (suppl): S304-10.","journal-title":"Rev Infect Dis"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2755","DOI":"10.1182\/blood.V80.11.2755.2755","article-title":"Tumor necrosis factor and interleukin-1 induce expression of the verocytotoxin receptor globotriaosylceramide on human endothelial cells: implication for the pathogenesis of the hemolytic uremic syndrome","volume":"80","author":"de Kar NCAJ, Van; LAH, Monnens; MA, Karmali; VWM, Van Hinsbergh","year":"1992","unstructured":"Van de Kar NCAJ, Monnens LAH, Karmali MA, Van Hinsbergh VWM. Tumor necrosis factor and interleukin-1 induce expression of the verocytotoxin receptor globotriaosylceramide on human endothelial cells: implication for the pathogenesis of the hemolytic uremic syndrome. Blood 1992; 80: 2755-64.","journal-title":"Blood"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"277","DOI":"10.4049\/jimmunol.153.1.277","article-title":"Peripheral blood mononuclear cells stimulated with C5a or lipopolysaccharide to synthesise equivalent levels of IL-13 mRNA show unequal IL-13 protein accumulation but similar polyribosome profiles","volume":"153","author":"Kasper, R.L.; Gehrke, L.","year":"1994","unstructured":"Kasper RL, Gehrke L. Peripheral blood mononuclear cells stimulated with C5a or lipopolysaccharide to synthesise equivalent levels of IL-13 mRNA show unequal IL-13 protein accumulation but similar polyribosome profiles. JImmunol 1994; 153: 277-86.","journal-title":"JImmunol"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1631","DOI":"10.1182\/blood.V76.8.1631.1631","article-title":"Recombinant C5a stimulate transcription of interleukin 1 (IL-1) and tumor necrosis factor: translational signal provided by the lipopolysaccharide or IL-1 itself","volume":"76","author":"Schindler, R.; Gelfand, J.A.; Dinarello, C.A.","year":"1990","unstructured":"Schindler R, Gelfand JA, Dinarello CA. Recombinant C5a stimulate transcription of interleukin 1 (IL-1) and tumor necrosis factor: translational signal provided by the lipopolysaccharide or IL-1 itself. Blood 1990; 76: 1631-8.","journal-title":"Blood"},{"key":"ref_31","first-page":"889","article-title":"Regulation of interferon-y mRNA in a cytolytic T cell clone: Ca+-induced transcription followed by mRNA stablization through activation of protein kinase C or increase in cAMP","volume":"25","author":"Kaldy, P.; Schmitt-Verhulst, A.-M.","year":"1995","unstructured":"Kaldy P, Schmitt-Verhulst A-M. Regulation of interferon-y mRNA in a cytolytic T cell clone: Ca+-induced transcription followed by mRNA stablization through activation of protein kinase C or increase in cAMP. Eur 7 Immunol 1995; 25: 889-5."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2054","DOI":"10.1128\/IAI.63.5.2054-2061.1995","article-title":"Immunoglobulin subclass distribution and dynamics of Shigella-specific antibody responses in serum and stool samples in shigellosis","volume":"63","author":"Islam, D.; Wretlind, B.; Ryd, M.; Lindberg, A.A.; Christensson, B.","year":"1995","unstructured":"Islam D, Wretlind B, Ryd M, Lindberg AA, Christensson B. Immunoglobulin subclass distribution and dynamics of Shigella-specific antibody responses in serum and stool samples in shigellosis. 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