{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T02:52:35Z","timestamp":1790909555282,"version":"4.1.0"},"reference-count":16,"publisher":"BMJ","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"published-print":{"date-parts":[[1996,8]]},"abstract":"<jats:p>BACKGROUND: While it is clear that luminal epidermal growth factor (EGF) stimulates repair of the damaged bowel, its significance in maintaining normal gut growth remains uncertain. If EGF is important in maintaining normal gut growth, the EGF receptor (EGF-R) should be present on the apical (luminal) surface in addition to the basolateral surface. AIMS\/SUBJECTS\/METHODS: This study examined the distribution of the EGF-R in the epithelium throughout the human gastro-intestinal tract using immunohistochemistry, electron microscopy, and western blotting of brush border preparations. RESULTS: Immunostaining of the oesophagus showed circumferential EGF-R positivity in the cells of the basal portions of the stratified squamous epithelium but surface cells were EGF-R negative. In the normal stomach, small intestine, and colon, immunostaining localised the receptor to the basolateral surface with the apical membranes being consistently negative. EGF-R positivity within the small intestine appeared to be almost entirely restricted to the proliferative (crypt) region. Western blotting demonstrated a 170 kDa protein in whole tissue homogenates but not in the brush border vesicle preparations. CONCLUSIONS: As the EGF-R is located only on the basolateral surfaces in the normal adult gastrointestinal tract, the major role of luminal EGF is probably to stimulate repair rather than to maintain normal gut growth.<\/jats:p>","DOI":"10.1136\/gut.39.2.262","type":"journal-article","created":{"date-parts":[[2007,9,18]],"date-time":"2007-09-18T17:41:31Z","timestamp":1190137291000},"page":"262-266","source":"Crossref","is-referenced-by-count":132,"title":["The epidermal growth factor receptor (EGF-R) is present on the basolateral, but not the apical, surface of enterocytes in the human gastrointestinal tract."],"prefix":"10.1136","volume":"39","author":[{"given":"R J","family":"Playford","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A M","family":"Hanby","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Gschmeissner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L P","family":"Peiffer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N A","family":"Wright","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"McGarrity","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[1996,8,1]]},"reference":[{"key":"ref_1","first-page":"408","article-title":"Immunohistochemical localisation of urogastrone to human duodenal and submaxillary glands","volume-title":"Gut","volume":"19","author":"Heitz, P.U.; Kasper, M.; Van Norden, S.; Polak, J.M.; Gregory, H.; Pearse, A.G.E.","year":"1978","unstructured":"Heitz PU, Kasper M, Van Norden S, Polak JM, Gregory H, Pearse AGE. Immunohistochemical localisation of urogastrone to human duodenal and submaxillary glands. Gut 1978 19: 408-13."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1136\/gut.28.5.573","article-title":"Intravenous but not intragastric urogastrone (EGF) is trophic to the intestine of parenterally fed rats","volume":"28","author":"Goodlad, R.A.; Wilson, T.J.G.; Lenton, W.; Gregory, H.; McClagh, K.G.; Wright, N.A.","year":"1987","unstructured":"Goodlad RA, Wilson TJG, Lenton W, Gregory H, McClagh KG, Wright NA. Intravenous but not intragastric urogastrone (EGF) is trophic to the intestine of parenterally fed rats. Gut 1987; 28: 573-82.","journal-title":"Gut"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1097\/00004836-199206001-00022","article-title":"Protection of gastric mucosa against ethanol injury by intragastric bolus administration of epidermal growth factor combined with hydroxypropylcellulose","volume":"14","author":"Itoh, M.; Imai, S.; Kawai, T.; Katsumi, K.; Yokochi, K.; Takeuchi, T.","year":"1992","unstructured":"Itoh M, Imai S, Kawai T, Katsumi K, Yokochi K, Takeuchi T. Protection of gastric mucosa against ethanol injury by intragastric bolus administration of epidermal growth factor combined with hydroxypropylcellulose. J Clin Gastroenterol 1992; 14: S 127-30.","journal-title":"J Clin Gastroenterol"},{"key":"ref_4","first-page":"284","article-title":"Immunohistochemical detection of the epidermal growth factor receptor in parrafin-embedded tissue","volume":"164","author":"Gullick, W.J.; Hughes, C.M.; Mellon, K.; Neal, D.E.; Lemoine, N.R.","year":"1991","unstructured":"Gullick WJ, Hughes CM, Mellon K, Neal DE, Lemoine NR. Immunohistochemical detection of the epidermal growth factor receptor in parrafin-embedded tissue. Jf Pathol 1991; 164: 284-9.","journal-title":"Jf Pathol"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/0005-2736(78)90440-6","article-title":"A modified procedure for the rapid preparation of efficiently transporting vesicles from small intestine brush border membranes","volume":"506","year":"1978","unstructured":"Kessler M, Acuto 0, Storelli C, Murer H, Muller M, Semenza G. A modified procedure for the rapid preparation of efficiently transporting vesicles from small intestine brush border membranes. Biochem Biophys Acta 1978; 506: 136-54.","journal-title":"Biochem Biophys Acta"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1016\/0005-2736(84)90047-6","article-title":"Studies of Fe3+ transport across isolated intestinal brush border membrane of the mouse","volume":"772","author":"Simpson, R.J.; Peters, T.J.","year":"1984","unstructured":"Simpson RJ, Peters TJ. Studies of Fe3+ transport across isolated intestinal brush border membrane of the mouse. Biochem Biophys Acta 1984; 772: 220-6.","journal-title":"Biochem Biophys Acta"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1016\/0003-2697(73)90523-X","article-title":"A simplified method for the quantitative assay of small amounts of protein in biological material","volume":"51","author":"Schacterle, G.; Pollack, R.","year":"1973","unstructured":"Schacterle G, Pollack R. A simplified method for the quantitative assay of small amounts of protein in biological material. Anal Biochem 1973; 51: 654-5.","journal-title":"Anal Biochem"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/0016-5085(95)90012-8","article-title":"EGF is digested to smaller, less active, forms in acidic gastric juice","volume":"108","author":"T, Playford R.J.Marchbank; J, Calam; FH, Hansen","year":"1995","unstructured":"Playford RJ. Marchbank T, Calam J, Hansen FH. EGF is digested to smaller, less active, forms in acidic gastric juice. Gastroenterology 1995; 108: 92-101.","journal-title":"Gastroenterology"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1016\/0140-6736(93)93057-8","article-title":"Effect of luminal growth factor preservation on intestinal growth","volume":"341","author":"Playford, R.J.; Watanaba, P.; Woodman, A.C.; Deprez, P.H.; Calam, J.","year":"1993","unstructured":"Playford RJ, Watanaba P, Woodman AC, Deprez PH, Calam J. Effect of luminal growth factor preservation on intestinal growth. Lancet 1993; 341: 843-8.","journal-title":"Lancet"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1278","DOI":"10.1016\/0016-5085(94)90528-2","article-title":"Developmental regulation of epidermal growth factor receptor kinase in rat intestine","volume":"107","author":"Thompson, J.F.; Van Den Berg, M.; Stokkers, P.C.F.","year":"1994","unstructured":"Thompson JF, Van Den Berg M, Stokkers PCF. Developmental regulation of epidermal growth factor receptor kinase in rat intestine. Gastroenterology 1994; 107: 1278-87.","journal-title":"Gastroenterology"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1071\/RD9920183","article-title":"Characterisation and localisation of the epidermal growth factor receptor in the jejunum of the neonatal and weaned pig","volume":"4","author":"Kelly, D.; McFadyen, M.; King, T.P.; Morgan, P.J.","year":"1992","unstructured":"Kelly D, McFadyen M, King TP, Morgan PJ. Characterisation and localisation of the epidermal growth factor receptor in the jejunum of the neonatal and weaned pig. Reprod Fertil Dev 1992; 4: 183-9.","journal-title":"Reprod Fertil Dev"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1136\/gut.33.4.439","article-title":"Epidermal growth-factor receptors in the oesophagus","volume":"33","author":"Jankowski, J.; Murphy, S.; Coghill, G.; Grant, A.; Wormsley, K.G.; Sanders, D.S.A.","year":"1992","unstructured":"Jankowski J, Murphy S, Coghill G, Grant A, Wormsley KG, Sanders DSA, et al. Epidermal growth-factor receptors in the oesophagus. Gut 1992; 33: 439-43.","journal-title":"Gut"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1042\/cs0820581","article-title":"Growth regulatory peptides in gastric mucosa","volume":"82","author":"Jankowski, J.; Al-Rawi, H.J.; Johnston, D.A.; Hopwood, D.; Filipe, M.I.; Coghill, G.","year":"1992","unstructured":"Jankowski J, Al-Rawi HJ, Johnston DA, Hopwood D, Filipe MI, Coghill G, et al. Growth regulatory peptides in gastric mucosa. 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Cell Tissue Res 1994; 278: 439-50.","journal-title":"Cell Tissue Res"},{"key":"ref_15","first-page":"1909","article-title":"Electron microscopy evidence for epidermal growth factor receptor (EGF-R) like immunoreactivity associated with the basolateral surface of gastric pariental cells","volume":"37","author":"Mori, S.; Morishita, Y.; Sakai, K.; Kurimoto, S.; Okamoto, M.; Kawamoto, T.","year":"1987","unstructured":"Mori S, Morishita Y, Sakai K, Kurimoto S, Okamoto M, Kawamoto T, et al. Electron microscopy evidence for epidermal growth factor receptor (EGF-R) like immunoreactivity associated with the basolateral surface of gastric pariental cells. Acta PatholJ'pn 1987; 37: 1909-17.","journal-title":"Acta PatholJ'pn"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1136\/gut.37.5.595","article-title":"The role of peptides in mucosal integrity and repair","volume":"37","author":"Playford, R.J.","year":"1995","unstructured":"Playford RJ. The role of peptides in mucosal integrity and repair. 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