{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T13:27:21Z","timestamp":1762176441836,"version":"3.44.0"},"reference-count":29,"publisher":"BMJ","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"accepted":{"date-parts":[[1997,1,28]]},"published-print":{"date-parts":[[1997,8,1]]},"abstract":"<jats:p>\n                  <jats:italic>Background and aims<\/jats:italic>\u2014To gain further insight into the role of the extracellular matrix during healing of peptic ulcers, sequential changes of procollagen expression were studied over 30 days of ulcer healing.<\/jats:p>\n               <jats:p>\n                  <jats:italic>Materials and methods<\/jats:italic>\u2014Procollagens \u03b11(I), \u03b11(III), and \u03b11(IV) RNA and their polypeptides were assessed in acetic acid induced rat gastric ulcers by in situ hybridisation and immunohistochemistry.<\/jats:p>\n               <jats:p>\n                  <jats:italic>Results<\/jats:italic>\u2014Three days after ulcer induction, intense hybridisation signals were obtained with all probes, with procollagen \u03b11(I) showing the highest transcript levels. Procollagen gene expression remained elevated up to day 15, but was reduced to initial low levels on day 30. Immunohistochemical staining documented increased deposition of the three procollagen types parallel to their respective transcript levels, again with type I showing the earliest and the most prominent deposits. The highest procollagen transcript levels were found in the intact submucosa surrounding the ulcer margins, followed by the muscularis propria and the serosa, with the lamina propria exhibiting the lowest transcript levels.<\/jats:p>\n               <jats:p>\n                  <jats:italic>Conclusion<\/jats:italic>\u2014The procollagens studied are regulated differentially at the transcriptional and post-transcriptional levels. The early onset and long duration of procollagen expression as well as the involvement of all layers of the gastric wall points to their central structural and functional role in gastric ulcer healing.<\/jats:p>","DOI":"10.1136\/gut.41.2.187","type":"journal-article","created":{"date-parts":[[2010,6,30]],"date-time":"2010-06-30T12:46:12Z","timestamp":1277901972000},"page":"187-194","source":"Crossref","is-referenced-by-count":12,"title":["Gastric ulcer healing in the rat: kinetics and localisation of de novo procollagen synthesis"],"prefix":"10.1136","volume":"41","author":[{"given":"M","family":"Shahin","sequence":"first","affiliation":[{"name":"aDepartment of Medicine B, University of M\u00fcnster, Germany, \n\nbDepartment of Gastroenterology, Free University of Berlin, Germany, \n\ncInstitute of Pathology, University of Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Gillessen","sequence":"additional","affiliation":[{"name":"aDepartment of Medicine B, University of M\u00fcnster, Germany, \n\nbDepartment of Gastroenterology, Free University of Berlin, Germany, \n\ncInstitute of Pathology, University of Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Pohle","sequence":"additional","affiliation":[{"name":"aDepartment of Medicine B, University of M\u00fcnster, Germany, \n\nbDepartment of Gastroenterology, Free University of Berlin, Germany, \n\ncInstitute of Pathology, University of Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Weber","sequence":"additional","affiliation":[{"name":"aDepartment of Medicine B, University of M\u00fcnster, Germany, \n\nbDepartment of Gastroenterology, Free University of Berlin, Germany, \n\ncInstitute of Pathology, University of Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D","family":"Schuppan","sequence":"additional","affiliation":[{"name":"aDepartment of Medicine B, University of M\u00fcnster, Germany, \n\nbDepartment of Gastroenterology, Free University of Berlin, Germany, \n\ncInstitute of Pathology, University of Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"Herbst","sequence":"additional","affiliation":[{"name":"aDepartment of Medicine B, University of M\u00fcnster, Germany, \n\nbDepartment of Gastroenterology, Free University of Berlin, Germany, \n\ncInstitute of Pathology, University of Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W","family":"Domschke","sequence":"additional","affiliation":[{"name":"aDepartment of Medicine B, University of M\u00fcnster, Germany, \n\nbDepartment of Gastroenterology, Free University of Berlin, Germany, \n\ncInstitute of Pathology, University of Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1997,8,1]]},"reference":[{"key":"2025090714545906000_41.2.187.1","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/0016-5085(94)90157-0","article-title":"Persistent lack of somatostatin receptors of gastric mucosa in healing ulcers in rat.","volume":"107","author":"Reubi","year":"1994","journal-title":"Gastroenterology"},{"key":"2025090714545906000_41.2.187.2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1994.1925"},{"key":"2025090714545906000_41.2.187.3","doi-asserted-by":"crossref","unstructured":"Tarnawski A (1993) Cellular mechanisms of gastric ulcer healing. in The stomach. 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