{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T22:04:01Z","timestamp":1791497041647,"version":"4.3.4"},"reference-count":36,"publisher":"BMJ","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"accepted":{"date-parts":[[1997,6,4]]},"published-print":{"date-parts":[[1997,11,1]]},"abstract":"<jats:p>\n                  <jats:italic>Background<\/jats:italic>\u2014Recent reports have described a modulating influence of nitric oxide (NO) on intestinal mucosal permeability and have implicated a role for mast cells in this NO mediated process.<\/jats:p>\n               <jats:p>\n                  <jats:italic>Aims<\/jats:italic>\u2014To assess further the contribution of mast cells to the mucosal permeability changes elicited by the NO synthase (NOS) inhibitor N<jats:sup>G<\/jats:sup>-nitro-<jats:sc>l<\/jats:sc>-arginine methylester (<jats:sc>l-NAME<\/jats:sc>), using mast cell deficient (W\/W<jats:sup>V<\/jats:sup>) and mast cell replete mice (+\/+).<\/jats:p>\n               <jats:p>\n                  <jats:italic>Methods<\/jats:italic>\u2014Chromium-51 EDTA clearance (from blood to jejunal lumen), jejunal NOS and myeloperoxidase (MPO) activities, and plasma nitrate\/nitrite levels were monitored.<\/jats:p>\n               <jats:p>\n                  <jats:italic>Results<\/jats:italic>\u2014The increased EDTA clearance elicited by intraluminal <jats:sc>l-NAME<\/jats:sc> in W\/W<jats:sup>V<\/jats:sup> mice (4.4-fold) was significantly greater than the response observed in control (+\/+) mice (1.8-fold). The exacerbated response in W\/W<jats:sup>v<\/jats:sup> mice was greatly attenuated by pretreatment with either dexamethasone (1.3-fold) or the selective inducible NOS inhibitor, aminoguanidine (1.4-fold), and partially attenuated by the mast cell stabiliser, lodoxamide (2.9-fold). Jejunal inducible NOS activity was significantly higher in W\/W<jats:sup>V<\/jats:sup> than in +\/+ mice, while jejunal MPO was lower in W\/W<jats:sup>V<\/jats:sup> mice than in +\/+ mice, suggesting that the higher inducible NOS in W\/W<jats:sup>V<\/jats:sup> does not result from the recruitment of inflammatory cells into the gut. The higher inducible NOS activity in the jejunum of W\/W<jats:sup>V<\/jats:sup> was significantly reduced by dexamethasone treatment.<\/jats:p>\n               <jats:p>\n                  <jats:italic>Conclusions<\/jats:italic>\u2014Our results suggest that mast cells normally serve to inhibit inducible NOS activity tonically in the gut and that inhibitors of NOS elicit a larger permeability response when this tonic inhibitory influence is released by mast cell depletion.<\/jats:p>","DOI":"10.1136\/gut.41.5.636","type":"journal-article","created":{"date-parts":[[2010,6,30]],"date-time":"2010-06-30T12:46:12Z","timestamp":1277901972000},"page":"636-641","source":"Crossref","is-referenced-by-count":15,"title":["Enhanced mucosal permeability and nitric oxide synthase activity in jejunum of mast cell deficient mice"],"prefix":"10.1136","volume":"41","author":[{"given":"S","family":"Komatsu","sequence":"first","affiliation":[{"name":"Department of Physiology, LSU Medical Center, 1501 Kings Highway, Shreveport, Louisiana 71130\u20133932, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M B","family":"Grisham","sequence":"additional","affiliation":[{"name":"Department of Physiology, LSU Medical Center, 1501 Kings Highway, Shreveport, Louisiana 71130\u20133932, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J M","family":"Russell","sequence":"additional","affiliation":[{"name":"Department of Physiology, LSU Medical Center, 1501 Kings Highway, Shreveport, Louisiana 71130\u20133932, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D N","family":"Granger","sequence":"additional","affiliation":[{"name":"Department of Physiology, LSU Medical Center, 1501 Kings Highway, Shreveport, Louisiana 71130\u20133932, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[1997,11,1]]},"reference":[{"key":"2025090714164569000_41.5.636.1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(92)90643-Y"},{"key":"2025090714164569000_41.5.636.2","doi-asserted-by":"crossref","unstructured":"Salvemini D, Masini E, Pistelli A, Mannaioni PF, Vane JR. 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