{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:09:53Z","timestamp":1791216593971,"version":"4.1.0"},"reference-count":44,"publisher":"BMJ","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"accepted":{"date-parts":[[1997,7,31]]},"published-print":{"date-parts":[[1998,1,1]]},"abstract":"<jats:p>\n                    <jats:italic>Background<\/jats:italic>\n                    \u2014Increased concentrations of 5-hydroxytryptamine (5-HT) can be detected in the systemic circulation after a meal and may be involved in the physiological control of gastrointestinal motility. Abnormalities of 5-HT release after a meal might explain some of the postprandial symptoms associated with the irritable bowel syndrome (IBS).\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Aim<\/jats:italic>\n                    \u2014To investigate the effect of a standard meal on plasma 5-HT and urinary 5-hydroxyindole acetic acid (5-HIAA) concentrations in patients with diarrhoea predominant IBS and in healthy volunteers.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Methods<\/jats:italic>\n                    \u2014After an overnight fast, six volunteers and five patients with IBS were given a carbohydrate-rich meal. Blood and urine samples were taken before and for four hours after the meal. Platelet-poor plasma 5-HT and urinary 5-HIAA were analysed by reversed phase high performance liquid chromatography with fluorometric detection. 5-HIAA was expressed as a ratio with urinary creatinine concentration, which was measured by spectrophotometry.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Results<\/jats:italic>\n                    \u2014During the four hour postprandial period, 5-HT concentrations were significantly higher in patients with IBS than in healthy volunteers at 0.5 hours (p&lt;0.05), 2 hours (p&lt;0.05) and 2.5 hours (p&lt;0.05). 5-HT was not detected in the plasma in the fasting state in patients or volunteers. Median peak 5-HT in patients with IBS (359 (198\u2013796) nmol\/l) was significantly greater than volunteers (83 (7\u2013190)) (p&lt;0.05). \u201cArea under the curve\u201d for 5-HT detection was greater for patients with IBS (317 (138\u2013771)) than for healthy volunteers (51 (4\u2013129); p&lt;0.05).The duration of the 5-HT peak was significantly longer in patients with IBS (3 (1\u20133) hours) than in the healthy volunteers (1 (1\u20131) hours; p&lt;0.01). Postprandial urinary median 5-HIAA values in controls (5.6 (5.5\u20135.8) \u03bcmol\/mmol creatinine) and patients with IBS (3.0 (2.5\u20136.8) \u03bcmol\/mmol creatinine) were not significantly different from preprandial values (controls: 5.9 (5.5\u20136.6) \u03bcmol\/mmol creatinine; patients with IBS: (6.2 (2.4\u20139.3) \u03bcmol\/mmol creatinine).\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Conclusion<\/jats:italic>\n                    \u2014These findings indicate that there may be a difference in the way that 5-HT is released in patients with diarrhoea predominant IBS, and could suggest a possible role for 5-HT in the postprandial symptoms of these patients.\n                  <\/jats:p>","DOI":"10.1136\/gut.42.1.42","type":"journal-article","created":{"date-parts":[[2010,6,30]],"date-time":"2010-06-30T12:46:12Z","timestamp":1277901972000},"page":"42-46","source":"Crossref","is-referenced-by-count":247,"title":["Postprandial plasma 5-hydroxytryptamine in diarrhoea predominant irritable bowel syndrome: a pilot study"],"prefix":"10.1136","volume":"42","author":[{"given":"C P","family":"Bearcroft","sequence":"first","affiliation":[{"name":"aDigestive Diseases Research Centre and St Bartholomew\u2019s and the Royal London School of Medicine and Dentistry, Turner Street, London E1 2AD, UK, \n\nbDepartment of Medicine, St Bartholomew\u2019s and the Royal London School of Medicine and Dentistry"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Perrett","sequence":"additional","affiliation":[{"name":"aDigestive Diseases Research Centre and St Bartholomew\u2019s and the Royal London School of Medicine and Dentistry, Turner Street, London E1 2AD, UK, \n\nbDepartment of Medicine, St Bartholomew\u2019s and the Royal London School of Medicine and Dentistry"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M J G","family":"Farthing","sequence":"additional","affiliation":[{"name":"aDigestive Diseases Research Centre and St Bartholomew\u2019s and the Royal London School of Medicine and Dentistry, Turner Street, London E1 2AD, UK, \n\nbDepartment of Medicine, St Bartholomew\u2019s and the Royal London School of Medicine and Dentistry"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[1998,1,1]]},"reference":[{"key":"2025121308485127000_42.1.42.1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(92)91789-7"},{"key":"2025121308485127000_42.1.42.2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.35.2.203"},{"key":"2025121308485127000_42.1.42.3","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(87)90434-3"},{"key":"2025121308485127000_42.1.42.4","doi-asserted-by":"publisher","DOI":"10.1016\/0022-3999(94)00126-P"},{"key":"2025121308485127000_42.1.42.5","doi-asserted-by":"publisher","DOI":"10.1136\/pgmj.50.585.416"},{"key":"2025121308485127000_42.1.42.6","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1113\/jphysiol.1960.sp006532","article-title":"Tissue 5-hydroxytryptamine and urinary 5-hydroxyindoleacetic acid after partial or total removal of the gastrointestinal tract in the rat.","volume":"153","author":"Bertaccini","year":"1960","journal-title":"J Physiol (Lond)"},{"key":"2025121308485127000_42.1.42.7","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1111\/j.2042-7158.1959.tb12603.x","article-title":"Observations on the release and turnover rate of 5-hydroxytryptamine in the gastrointestinal tract.","volume":"11","author":"Erspamer","year":"1959","journal-title":"J Pharm Pharmacol"},{"key":"2025121308485127000_42.1.42.8","doi-asserted-by":"publisher","DOI":"10.1007\/BF01965326"},{"key":"2025121308485127000_42.1.42.9","first-page":"49P","article-title":"Does endogenous neuronal 5-hydroxytryptamine influence canine intestinal motility [abstract]?","volume":"376","author":"Davidson","year":"1986","journal-title":"J Physiol (Lond)"},{"key":"2025121308485127000_42.1.42.10","first-page":"444","article-title":"Observations concerning the action of 5-hydroxytryptamine on the peristaltic reflex.","volume":"13","author":"Bulbring","year":"1958","journal-title":"Br J Pharmacol"},{"key":"2025121308485127000_42.1.42.11","doi-asserted-by":"publisher","DOI":"10.1136\/gut.35.4.496"},{"key":"2025121308485127000_42.1.42.12","doi-asserted-by":"publisher","DOI":"10.1007\/BF01536922"},{"key":"2025121308485127000_42.1.42.13","doi-asserted-by":"crossref","first-page":"562","DOI":"10.3109\/00365529509089790","article-title":"Ascending colon response to feeding: evidence for a 5-hydroxytryptamine-3 mechanism.","volume":"30","author":"Scolapio","year":"1995","journal-title":"Scand J Gastroenterol"},{"key":"2025121308485127000_42.1.42.14","doi-asserted-by":"crossref","first-page":"1972","DOI":"10.1016\/0016-5085(86)90269-6","article-title":"Significance of calcium for the prostaglandin E2-mediated secretory response to 5-hydroxytryptamine in the small intestine.","volume":"90","author":"Beubler","year":"1986","journal-title":"Gastroenterology"},{"key":"2025121308485127000_42.1.42.15","doi-asserted-by":"publisher","DOI":"10.1172\/JCI109862"},{"key":"2025121308485127000_42.1.42.16","first-page":"237","article-title":"Carcinoid tumours and syndrome.","volume":"14","author":"Feldman","year":"1987","journal-title":"Semin Oncol"},{"key":"2025121308485127000_42.1.42.17","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.294.6580.1129"},{"key":"2025121308485127000_42.1.42.18","doi-asserted-by":"crossref","first-page":"1184","DOI":"10.1016\/0016-5085(94)90008-6","article-title":"A 5-HT3 antagonist corrects the postprandial colonic hypertonic response in carcinoid diarrhoea.","volume":"106","author":"Von der Ohe","year":"1994","journal-title":"Gastroenterology"},{"key":"2025121308485127000_42.1.42.19","doi-asserted-by":"publisher","DOI":"10.3109\/00365527809181914"},{"key":"2025121308485127000_42.1.42.20","doi-asserted-by":"publisher","DOI":"10.1007\/BF01296048"},{"key":"2025121308485127000_42.1.42.21","first-page":"1","article-title":"Enterochromaffin cells and macrophages in ulcerative colitis and irritable colon.","volume":"8","author":"Ahonen","year":"1976","journal-title":"Ann Clin Res"},{"key":"2025121308485127000_42.1.42.22","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1111\/j.1365-2036.1993.tb00087.x","article-title":"Reduction of rectal sensitivity and post-prandial motility by granisetron, a 5-HT3 receptor antagonist in patients with irritable bowel syndrome.","volume":"7","author":"Prior","year":"1993","journal-title":"Aliment Pharmacol Ther"},{"key":"2025121308485127000_42.1.42.23","doi-asserted-by":"publisher","DOI":"10.1016\/S0025-6196(12)60797-6"},{"key":"2025121308485127000_42.1.42.24","doi-asserted-by":"publisher","DOI":"10.1016\/0026-0495(92)90141-V"},{"key":"2025121308485127000_42.1.42.25","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.2.6138.653"},{"key":"2025121308485127000_42.1.42.26","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.1130090105"},{"key":"2025121308485127000_42.1.42.27","first-page":"307","article-title":"The irritable colon syndrome: a study of the clinical features, predisposing causes, and prognosis in 130 cases.","volume":"31","author":"Chaudhary","year":"1962","journal-title":"Q J Med"},{"key":"2025121308485127000_42.1.42.28","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(87)90568-6"},{"key":"2025121308485127000_42.1.42.29","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(85)90406-0"},{"key":"2025121308485127000_42.1.42.30","doi-asserted-by":"crossref","first-page":"2117","DOI":"10.1093\/clinchem\/36.12.2117","article-title":"Determination of reference values for serotonin concentration in platelets of healthy newborns, children, adults, and elderly subjects by HPLC with electrochemical detection.","volume":"36","author":"Flachaire","year":"1990","journal-title":"Clin Chem"},{"key":"2025121308485127000_42.1.42.31","doi-asserted-by":"publisher","DOI":"10.1177\/000456328702400613"},{"key":"2025121308485127000_42.1.42.32","doi-asserted-by":"crossref","first-page":"862","DOI":"10.1038\/bjc.1990.395","article-title":"Cisplatin induced emesis: preliminary results indicative of changes in plasma levels of 5-hydroxytryptamine.","volume":"62","author":"Barnes","year":"1990","journal-title":"Br J Cancer"},{"key":"2025121308485127000_42.1.42.33","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1093\/clinchem\/30.1.131","article-title":"Simplified determination of serotonin in plasma by liquid chromatography with electrochemical detection.","volume":"30","author":"Tagari","year":"1984","journal-title":"Clin Chem"},{"key":"2025121308485127000_42.1.42.34","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(95)90447-6"},{"key":"2025121308485127000_42.1.42.35","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1993.2243"},{"key":"2025121308485127000_42.1.42.36","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1016\/S0016-5085(76)80488-X","article-title":"Validation and application of a radioimmunoassay for serotonin.","volume":"70","author":"Kellum","year":"1976","journal-title":"Gastroenterology"},{"key":"2025121308485127000_42.1.42.37","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/S0016-5085(85)80137-2","article-title":"Effect of a meal on human whole blood serotonin.","volume":"88","author":"Anderson","year":"1985","journal-title":"Gastroenterology"},{"key":"2025121308485127000_42.1.42.38","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199003223221204"},{"key":"2025121308485127000_42.1.42.39","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1992.242"},{"key":"2025121308485127000_42.1.42.40","doi-asserted-by":"publisher","DOI":"10.1002\/j.1552-4604.1993.tb05608.x"},{"key":"2025121308485127000_42.1.42.41","doi-asserted-by":"publisher","DOI":"10.1016\/0165-6147(88)90106-X"},{"key":"2025121308485127000_42.1.42.42","doi-asserted-by":"publisher","DOI":"10.1136\/gut.24.5.405"},{"key":"2025121308485127000_42.1.42.43","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1111\/j.1365-2036.1990.tb00458.x","article-title":"Colonic transit in man is slowed by ondansetron (GR38032F), a selective 5-hydroxytryptamine receptor (type 3) antagonist.","volume":"4","author":"Gore","year":"1990","journal-title":"Aliment Pharmacol Ther"},{"key":"2025121308485127000_42.1.42.44","doi-asserted-by":"crossref","unstructured":"Sanger GJ Wardle KA Shapcott S Yee KF (1991) Constipation evoked by 5-HT3 receptor antagonists. in Serotonin: molecular biology, receptors and functional effects. eds Fozard JR Saxena PR (Birkhauser Verlag, Basel), pp 381\u2013388.","DOI":"10.1007\/978-3-0348-7259-1_37"}],"container-title":["Gut"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/gut.42.1.42","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T16:49:00Z","timestamp":1765644540000},"score":1,"resource":{"primary":{"URL":"https:\/\/gut.bmj.com\/lookup\/doi\/10.1136\/gut.42.1.42"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,1,1]]},"references-count":44,"journal-issue":{"issue":"1","published-online":{"date-parts":[[1998,1,1]]},"published-print":{"date-parts":[[1998,1,1]]}},"alternative-id":["10.1136\/gut.42.1.42"],"URL":"https:\/\/doi.org\/10.1136\/gut.42.1.42","relation":{},"ISSN":["0017-5749","1468-3288"],"issn-type":[{"value":"0017-5749","type":"print"},{"value":"1468-3288","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,1,1]]}}}