{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T12:46:01Z","timestamp":1765629961584,"version":"3.48.0"},"reference-count":32,"publisher":"BMJ","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"accepted":{"date-parts":[[1997,6,24]]},"published-print":{"date-parts":[[1998,3,1]]},"abstract":"<jats:p>\n                    <jats:italic>Background<\/jats:italic>\n                    \u2014The bacterial chemotactic peptide\n                    <jats:italic>N-<\/jats:italic>\n                    formyl-methionine-leucine-phenylalanine (FMLP) is produced by enteric flora and is one of the factors implicated in contributing to inflammatory bowel disease. Expression of receptors for FMLP on human phagocytes (polymorphs and monocytes) is well established, but there is conflicting evidence regarding the potential expression of FMLP receptors on other cells within the mucosa, particularly the epithelial cells.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Aims<\/jats:italic>\n                    \u2014To map FMLP receptors within intestinal mucosa using several different experimental approaches.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Methods and results\u2014<\/jats:italic>\n                    Radioligand binding assays with\n                    <jats:sup>3<\/jats:sup>\n                    H-FMLP revealed no specific binding to primary cultured colonic enterocytes or to the cell line HT29, whereas neutrophils, as expected, exhibited specific binding with a Kd of 19 nM and approximately 2 \u00d7 10\n                    <jats:sup>4<\/jats:sup>\n                    receptors per cell. FITC labelled FMLP exhibited specific, displaceable binding on flow cytometry to neutrophils and monocytes but not to 10 gastrointestinal epithelial cell lines. Isolated lamina propria lymphocytes and peripheral blood lymphocytes exhibited no binding. To confirm the absence of receptors on epithelia, reverse transcription polymerase chain reaction for mRNA for the classic FMLP receptor was performed. While the presence of message was detected in activated peripheral blood phagocytes, it was not detected in epithelial cell lines. To exclude the possibility of FMLP binding to other receptors such as tachykinin receptors on epithelia, FITC labelled FMLP binding in tissue sections confirmed that the binding is subepithelial\u2014that is, in the lamina propria.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Conclusion<\/jats:italic>\n                    \u2014Receptors for FMLP are subepithelial and map to the lamina propria of the gastrointestinal mucosa.\n                  <\/jats:p>","DOI":"10.1136\/gut.42.3.374","type":"journal-article","created":{"date-parts":[[2010,6,30]],"date-time":"2010-06-30T12:46:12Z","timestamp":1277901972000},"page":"374-379","source":"Crossref","is-referenced-by-count":33,"title":["Mucosal subepithelial binding sites for the bacterial chemotactic peptide, formyl-methionyl-leucyl-phenylalanine (FMLP)"],"prefix":"10.1136","volume":"42","author":[{"given":"P","family":"Anton","sequence":"first","affiliation":[{"name":"Department of Medicine, Pharmacology, and Surgery, National University of Ireland, Cork, Republic of Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"O\u2019Connell","sequence":"additional","affiliation":[{"name":"Department of Medicine, Pharmacology, and Surgery, National University of Ireland, Cork, Republic of Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D","family":"O\u2019Connell","sequence":"additional","affiliation":[{"name":"Department of Medicine, Pharmacology, and Surgery, National University of Ireland, Cork, Republic of Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L","family":"Whitaker","sequence":"additional","affiliation":[{"name":"Department of Medicine, Pharmacology, and Surgery, National University of Ireland, Cork, Republic of Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G C","family":"O\u2019Sullivan","sequence":"additional","affiliation":[{"name":"Department of Medicine, Pharmacology, and Surgery, National University of Ireland, Cork, Republic of Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J K","family":"Collins","sequence":"additional","affiliation":[{"name":"Department of Medicine, Pharmacology, and Surgery, National University of Ireland, Cork, Republic of Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F","family":"Shanahan","sequence":"additional","affiliation":[{"name":"Department of Medicine, Pharmacology, and Surgery, National University of Ireland, Cork, Republic of Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1998,3,1]]},"reference":[{"key":"2025121304411090000_42.3.374.1","doi-asserted-by":"crossref","first-page":"121","DOI":"10.3109\/00365528809093861","article-title":"Production of peptides inducing chemotaxis and lysosomal enzyme release in human neutrophils by intestinal bacteria in vitro and in vivo.","volume":"23","author":"Chadwick","year":"1988","journal-title":"Scand J Gastroenterol"},{"key":"2025121304411090000_42.3.374.2","doi-asserted-by":"crossref","first-page":"5430","DOI":"10.1016\/S0021-9258(18)91029-X","article-title":"Purification and identification of formyl-methionyl-leucyl-phenylalanine as the major peptide neutrophil chemotactic factor produced by Escherichia coli.","volume":"259","author":"Marasco","year":"1984","journal-title":"J Biol Chem"},{"key":"2025121304411090000_42.3.374.3","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1111\/j.1440-1746.1990.tb01765.x","article-title":"Radioimmunoassay for formyl-methionyl-leucyl-phenylalanine (FMLP): I. 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