{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T05:45:10Z","timestamp":1791265510544,"version":"4.1.0"},"reference-count":41,"publisher":"BMJ","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"accepted":{"date-parts":[[1997,12,18]]},"published-print":{"date-parts":[[1998,5,1]]},"abstract":"<jats:p>\n                    <jats:italic>Background<\/jats:italic>\n                    \u2014Up to 15% of colorectal cancers are characterised by DNA microsatellite instability (MIN), shown by the presence of DNA replication errors (RERs).\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Aims<\/jats:italic>\n                    \u2014To identify pathological features that are discriminating for colorectal cancer (CRC) showing extensive MIN.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Subjects<\/jats:italic>\n                    \u2014A prospective series of 303 patients with CRC and no family history of either familial adenomatous polyposis or hereditary non-polyposis colorectal cancer.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Methods<\/jats:italic>\n                    \u2014DNA was extracted from fresh tissue samples and the presence of MIN was studied at nine loci that included TGF\u03b2RII, IGFIIR, and BAX. The 61 cases showing RERs were compared with 63 RER negative cases with respect to a comprehensive set of clinical and pathological variables. Predictive utility of the variables was tested by decision tree analysis.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Results<\/jats:italic>\n                    \u2014Twenty seven patients with CRC showed extensive RERs (three loci or more) (RER+) and 34 had limited RERs only (28 = one locus; 6 = two loci) (RER+\/\u2212), yielding a bimodal distribution. RER+ cancers differed from RER\u2212 and RER+\/\u2212 cases. Tumour type (adenocarcinoma, mucinous carcinoma, and undifferentiated carcinoma) (p=0.001), tumour infiltrating lymphocytes (p=0.001), and anatomical site (p=0.001) were the most significant of the discriminating variables. Algorithms developed by decision tree analysis allowed cases to be assigned to RER+ versus RER\u2212 and +\/\u2212 status with a global sensitivity of 81.5%, specificity of 96%, and overall accuracy of 93%.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Conclusion<\/jats:italic>\n                    \u2014Pathological examination of CRC allows assignment of RER+ status; assignment is specific and relatively sensitive. Conversely RER\u2212 and RER+\/\u2212 CRC are indistinguishable.\n                  <\/jats:p>","DOI":"10.1136\/gut.42.5.673","type":"journal-article","created":{"date-parts":[[2010,6,30]],"date-time":"2010-06-30T12:46:12Z","timestamp":1277901972000},"page":"673-679","source":"Crossref","is-referenced-by-count":358,"title":["Morphology of sporadic colorectal cancer with DNA replication errors"],"prefix":"10.1136","volume":"42","author":[{"given":"J R","family":"Jass","sequence":"first","affiliation":[{"name":"aDepartment of Pathology, \n\nbDepartment of Surgery, University of Queensland, \n\ncGlaxo Conjoint Gastroenterology Laboratory, \n\ndQueensland Health Pathology Services, Royal Brisbane Hospital, \n\neQueensland Institute of Medical Research and Centre for Statistics, University of Queensland, Queensland, Australia, \n\nfFirst Department of Surgery, Yamanashi Medical School, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K-A","family":"Do","sequence":"additional","affiliation":[{"name":"aDepartment of Pathology, \n\nbDepartment of Surgery, University of Queensland, \n\ncGlaxo Conjoint Gastroenterology Laboratory, \n\ndQueensland Health Pathology Services, Royal Brisbane Hospital, \n\neQueensland Institute of Medical Research and Centre for Statistics, University of Queensland, Queensland, Australia, \n\nfFirst Department of Surgery, Yamanashi Medical School, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L A","family":"Simms","sequence":"additional","affiliation":[{"name":"aDepartment of Pathology, \n\nbDepartment of Surgery, University of Queensland, \n\ncGlaxo Conjoint Gastroenterology Laboratory, \n\ndQueensland Health Pathology Services, Royal Brisbane Hospital, \n\neQueensland Institute of Medical Research and Centre for Statistics, University of Queensland, Queensland, Australia, \n\nfFirst Department of Surgery, Yamanashi Medical School, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Iino","sequence":"additional","affiliation":[{"name":"aDepartment of Pathology, \n\nbDepartment of Surgery, University of Queensland, \n\ncGlaxo Conjoint Gastroenterology Laboratory, \n\ndQueensland Health Pathology Services, Royal Brisbane Hospital, \n\neQueensland Institute of Medical Research and Centre for Statistics, University of Queensland, Queensland, Australia, \n\nfFirst Department of Surgery, Yamanashi Medical School, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Wynter","sequence":"additional","affiliation":[{"name":"aDepartment of Pathology, \n\nbDepartment of Surgery, University of Queensland, \n\ncGlaxo Conjoint Gastroenterology Laboratory, \n\ndQueensland Health Pathology Services, Royal Brisbane Hospital, \n\neQueensland Institute of Medical Research and Centre for Statistics, University of Queensland, Queensland, Australia, \n\nfFirst Department of Surgery, Yamanashi Medical School, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S P","family":"Pillay","sequence":"additional","affiliation":[{"name":"aDepartment of Pathology, \n\nbDepartment of Surgery, University of Queensland, \n\ncGlaxo Conjoint Gastroenterology Laboratory, \n\ndQueensland Health Pathology Services, Royal Brisbane Hospital, \n\neQueensland Institute of Medical Research and Centre for Statistics, University of Queensland, Queensland, Australia, \n\nfFirst Department of Surgery, Yamanashi Medical School, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Searle","sequence":"additional","affiliation":[{"name":"aDepartment of Pathology, \n\nbDepartment of Surgery, University of Queensland, \n\ncGlaxo Conjoint Gastroenterology Laboratory, \n\ndQueensland Health Pathology Services, Royal Brisbane Hospital, \n\neQueensland Institute of Medical Research and Centre 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