{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T03:56:51Z","timestamp":1764993411876,"version":"3.46.0"},"reference-count":15,"publisher":"BMJ","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"accepted":{"date-parts":[[1998,3,31]]},"published-print":{"date-parts":[[1998,10,1]]},"abstract":"<jats:p>\n                    <jats:italic>Background<\/jats:italic>\n                    \u2014CD3 and \u03b3\u03b4 cells in the rectal mucosa increase after local instillation of gluten in children with coeliac disease and in half of their siblings.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Aim<\/jats:italic>\n                    \u2014To establish an in vitro system for assessing immunological changes induced by gluten in the rectum.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Patients and Methods<\/jats:italic>\n                    \u2014Rectal biopsy specimens obtained from 13 treated coeliac children, nine of their siblings, and nine controls were cultured in vitro with a peptic-tryptic digest of gliadin or ovalbumin. CD3 and CD25 cells were counted, and the expression of adhesion molecules evaluated.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Results<\/jats:italic>\n                    \u2014In the lamina propria of coeliac biopsy samples cultured with gliadin, but not in those from controls, the expression of vascular cell adhesion molecule 1 (VCAM-1) was enhanced, and the number of CD25 cells was significantly higher than in those cultured in medium alone; the density of intraepithelial CD3 cells was also significantly higher. No differences were noted in coeliac biopsy specimens cultured with ovalbumin. A discriminant analysis allowed correct classification of all controls and all coeliacs but one, but three of nine siblings were allocated to the coeliac group.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Conclusions<\/jats:italic>\n                    \u2014Our data confirm that gliadin is able to activate cell mediated immunity in the rectal mucosa in coeliac patients and in a subset of their first degree relatives.\n                  <\/jats:p>","DOI":"10.1136\/gut.43.4.484","type":"journal-article","created":{"date-parts":[[2010,6,30]],"date-time":"2010-06-30T12:46:12Z","timestamp":1277901972000},"page":"484-489","source":"Crossref","is-referenced-by-count":15,"title":["Gliadin activates mucosal cell mediated immunity in cultured rectal mucosa from coeliac patients and a subset of their siblings"],"prefix":"10.1136","volume":"43","author":[{"given":"R","family":"Troncone","sequence":"first","affiliation":[{"name":"aDepartment of Pediatrics, University Federico II, Naples, Italy, \n\nbIstituto di Scienze dell\u2019Alimentazione CNR, Avellino, Italy, \n\ncLaboratory for Metabolism and Pathological Biochemistry, Istituto Superiore di Sanita\u2019, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G","family":"Mazzarella","sequence":"additional","affiliation":[{"name":"aDepartment of Pediatrics, University Federico II, Naples, Italy, \n\nbIstituto di Scienze dell\u2019Alimentazione CNR, Avellino, Italy, \n\ncLaboratory for Metabolism and Pathological Biochemistry, Istituto Superiore di Sanita\u2019, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N","family":"Leone","sequence":"additional","affiliation":[{"name":"aDepartment of Pediatrics, University Federico II, Naples, Italy, \n\nbIstituto di Scienze dell\u2019Alimentazione CNR, Avellino, Italy, \n\ncLaboratory for Metabolism and Pathological Biochemistry, Istituto Superiore di Sanita\u2019, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Mayer","sequence":"additional","affiliation":[{"name":"aDepartment of Pediatrics, University Federico II, Naples, Italy, \n\nbIstituto di Scienze dell\u2019Alimentazione CNR, Avellino, Italy, \n\ncLaboratory for Metabolism and Pathological Biochemistry, Istituto Superiore di Sanita\u2019, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"De Vincenzi","sequence":"additional","affiliation":[{"name":"aDepartment of Pediatrics, University Federico II, Naples, Italy, \n\nbIstituto di Scienze dell\u2019Alimentazione CNR, Avellino, Italy, \n\ncLaboratory for Metabolism and Pathological Biochemistry, Istituto Superiore di Sanita\u2019, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L","family":"Greco","sequence":"additional","affiliation":[{"name":"aDepartment of Pediatrics, University Federico II, Naples, Italy, \n\nbIstituto di Scienze dell\u2019Alimentazione CNR, Avellino, Italy, \n\ncLaboratory for Metabolism and Pathological Biochemistry, Istituto Superiore di Sanita\u2019, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Auricchio","sequence":"additional","affiliation":[{"name":"aDepartment of Pediatrics, University Federico II, Naples, Italy, \n\nbIstituto di Scienze dell\u2019Alimentazione CNR, Avellino, Italy, \n\ncLaboratory for Metabolism and Pathological Biochemistry, Istituto Superiore di Sanita\u2019, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1998,10,1]]},"reference":[{"key":"2025120518402185000_43.4.484.1","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(92)91819-P"},{"key":"2025120518402185000_43.4.484.2","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-3955(05)70410-7"},{"key":"2025120518402185000_43.4.484.3","doi-asserted-by":"publisher","DOI":"10.1136\/gut.34.9.1225"},{"key":"2025120518402185000_43.4.484.4","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1111\/j.1365-2249.1993.tb03396.x","article-title":"Time-course of adhesion molecule expression in rectal mucosa of gluten- sensitive subjects after gluten challenge.","volume":"92","author":"Ensari","year":"1993","journal-title":"Clin Exp Immunol"},{"key":"2025120518402185000_43.4.484.5","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/0016-5085(89)91411-X","article-title":"Studies of intestinal lymphoid tissue. 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