{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T23:01:25Z","timestamp":1765666885511,"version":"3.44.0"},"reference-count":15,"publisher":"BMJ","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"accepted":{"date-parts":[[1999,6,2]]},"published-print":{"date-parts":[[1999,11,1]]},"abstract":"<jats:sec>\n                  <jats:title>BACKGROUND<\/jats:title>\n                  <jats:p>Animal studies have shown that the neuromuscular structures on the luminal side of the colonic circular muscle coordinate circular muscle activity. These structures have been identified by electron microscopy in the normal human colon, but have never been thoroughly studied in patients with acquired intestinal hypoganglionosis.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>AIMS<\/jats:title>\n                  <jats:p>To perform histological, immunocytochemical, and electron microscopic examinations of the colon of a patient with acquired intestinal hypoganglionosis presenting as megacolon.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>PATIENT<\/jats:title>\n                  <jats:p>A 32 year old man with a one year history of constipation and abdominal distention, a massively dilated ascending and transverse colon, and a normal calibre rectum and descending and sigmoid colon. He had a high titre of circulating serum anti-neuronal nuclear antibodies.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec sec-type=\"methods\">\n                  <jats:title>METHODS<\/jats:title>\n                  <jats:p>Histology, immunocytochemistry (for neurofilaments, neurone specific enolase, synaptophysin, glial fibrillar acidic protein, S100 protein, and smooth muscle \u03b1-actin), and electron microscopic examinations on the resected colon.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>RESULTS<\/jats:title>\n                  <jats:p>The number of ganglion cells and nerve trunks was decreased throughout the colon. Disruption of the neural network and a loss of interstitial cells of Cajal were observed on the luminal side of the circular muscle; in their place, the non-dilated colon contained a hypertrophic fibromuscular layer.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>CONCLUSIONS<\/jats:title>\n                  <jats:p>Striking architectural alterations occurred at the site regarded as the source of the coordination of colonic circular muscle activity in an adult patient with acquired intestinal hypoganglionosis presenting as megacolon.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1136\/gut.45.5.775","type":"journal-article","created":{"date-parts":[[2010,6,30]],"date-time":"2010-06-30T12:46:12Z","timestamp":1277901972000},"page":"775-779","source":"Crossref","is-referenced-by-count":59,"title":["Loss of interstitial cells and a fibromuscular layer on the luminal side of the colonic circular muscle presenting as megacolon in an adult patient"],"prefix":"10.1136","volume":"45","author":[{"given":"M S","family":"Faussone-Pellegrini","sequence":"first","affiliation":[{"name":"aDepartment of Anatomy, Histology and Forensic Medicine, University of Florence, Florence, Italy, \n\nbDepartment of Pathological Anatomy, University of Milan, Milan, Italy, \n\ncDepartment of Gastroenterology, Institute of Medical Sciences of the University, IRCCS-Ospedale Maggiore, Milan, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P","family":"Fociani","sequence":"additional","affiliation":[{"name":"aDepartment of Anatomy, Histology and Forensic Medicine, University of Florence, Florence, Italy, \n\nbDepartment of Pathological Anatomy, University of Milan, Milan, Italy, \n\ncDepartment of Gastroenterology, Institute of Medical Sciences of the University, IRCCS-Ospedale Maggiore, Milan, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Buffa","sequence":"additional","affiliation":[{"name":"aDepartment of Anatomy, Histology and Forensic Medicine, University of Florence, Florence, Italy, \n\nbDepartment of Pathological Anatomy, University of Milan, Milan, Italy, \n\ncDepartment of Gastroenterology, Institute of Medical Sciences of the University, IRCCS-Ospedale Maggiore, Milan, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G","family":"Basilisco","sequence":"additional","affiliation":[{"name":"aDepartment of Anatomy, Histology and Forensic Medicine, University of Florence, Florence, Italy, \n\nbDepartment of Pathological Anatomy, University of Milan, Milan, Italy, \n\ncDepartment of Gastroenterology, Institute of Medical Sciences of the University, IRCCS-Ospedale Maggiore, Milan, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1999,11,1]]},"reference":[{"key":"2025090711504614000_45.5.775.1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(97)70151-3"},{"key":"2025090711504614000_45.5.775.2","doi-asserted-by":"publisher","DOI":"10.1139\/y90-218"},{"key":"2025090711504614000_45.5.775.3","doi-asserted-by":"crossref","first-page":"C215","DOI":"10.1152\/ajpcell.1987.252.2.C215","article-title":"Origin and propagation of electrical slow waves in circular muscle of canine proximal colon.","volume":"252","author":"Smith","year":"1987","journal-title":"Am J Physiol"},{"key":"2025090711504614000_45.5.775.4","doi-asserted-by":"publisher","DOI":"10.1139\/cjpp-75-2-112"},{"key":"2025090711504614000_45.5.775.5","doi-asserted-by":"publisher","DOI":"10.1136\/gut.40.2.188"},{"key":"2025090711504614000_45.5.775.6","doi-asserted-by":"crossref","first-page":"1937","DOI":"10.1212\/WNL.45.10.1937","article-title":"Guidelines on the detection of paraneoplastic anti-neuronal-specific antibodies: Report from the Workshop to the Fourth Meeting of the International Society of Neuro-immunology on Paraneoplastic Neurological Disease, held October 22\u201323, 1994, in Rotterdam, The Netherlands.","volume":"45","author":"Moll","year":"1995","journal-title":"Neurology"},{"key":"2025090711504614000_45.5.775.7","first-page":"G1309","article-title":"Regional and transmural density of interstitial cells of Cajal in human colon and rectum.","volume":"275","author":"Hagger","year":"1998","journal-title":"Am J Physiol"},{"key":"2025090711504614000_45.5.775.8","first-page":"G381","article-title":"Neural injury, repair, and adaptation in the GI tract. IV. Pathophysiology of GI motility related to interstitial cells of Cajal.","volume":"275","author":"Huizinga","year":"1998","journal-title":"Am J Physiol"},{"key":"2025090711504614000_45.5.775.9","unstructured":"Phillips SF Pemberton JH (1997) Megacolon: congenital and acquired. in Gastrointestinal and liver disease. Pathophysiology\/diagnosis\/management. eds Feldman M Scharschmidt BF Sleisenger MH (Saunders, Philadelphia), pp 1810\u20131819."},{"key":"2025090711504614000_45.5.775.10","doi-asserted-by":"publisher","DOI":"10.1136\/gut.41.2.252"},{"key":"2025090711504614000_45.5.775.11","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(94)90546-0"},{"key":"2025090711504614000_45.5.775.12","first-page":"673","article-title":"The muscle coat of the lower esophageal sphincter in patients with achalasia and hypertensive sphincter. An electron microscopic study.","volume":"17","author":"Faussone-Pellegrini","year":"1985","journal-title":"J Submicrosc Cytol"},{"key":"2025090711504614000_45.5.775.13","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.1800840406"},{"key":"2025090711504614000_45.5.775.14","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(96)70057-4"},{"key":"2025090711504614000_45.5.775.15","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(96)00935-3"}],"container-title":["Gut"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/gut.45.5.775","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,7]],"date-time":"2025-09-07T18:50:54Z","timestamp":1757271054000},"score":1,"resource":{"primary":{"URL":"https:\/\/gut.bmj.com\/lookup\/doi\/10.1136\/gut.45.5.775"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,11,1]]},"references-count":15,"journal-issue":{"issue":"5","published-online":{"date-parts":[[1999,11,1]]},"published-print":{"date-parts":[[1999,11,1]]}},"alternative-id":["10.1136\/gut.45.5.775"],"URL":"https:\/\/doi.org\/10.1136\/gut.45.5.775","relation":{},"ISSN":["0017-5749","1468-3288"],"issn-type":[{"type":"print","value":"0017-5749"},{"type":"electronic","value":"1468-3288"}],"subject":[],"published":{"date-parts":[[1999,11,1]]}}}