{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,28]],"date-time":"2025-12-28T02:26:29Z","timestamp":1766888789536,"version":"3.48.0"},"reference-count":53,"publisher":"BMJ","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"accepted":{"date-parts":[[1999,6,2]]},"published-print":{"date-parts":[[1999,12,1]]},"abstract":"<jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>Paracellular permeability to solutes across the descending colon is much higher in cattle than sheep. This is a possible route for transmission of infective materials, such as scrapie prion.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>AIMS<\/jats:title>\n                    <jats:p>To compare the permeabilities of labelled scrapie prion protein and other macromolecules in bovine and ovine descending colons in vitro.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec sec-type=\"methods\">\n                    <jats:title>METHODS<\/jats:title>\n                    <jats:p>Using fresh slaughterhouse material, transepithelial fluxes of macromolecules across colonic mucosae mounted in Ussing chambers were measured by monitoring transport of either enzyme activity or radioactivity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      The comparative bovine to ovine permeability ratio of the probes increased with molecular weight: from 3.1 (0.13) for PEG400 to 10.67 (0.20) (p&lt;0.001) for PEG4000; and from 1.64 (0.17) for microperoxidase to 7.03 (0.20) (p&lt;0.001) for horseradish peroxidase (HRP). The permeability of\n                      <jats:sup>125<\/jats:sup>\n                      I-labelled inactivated Syrian hamster scrapie prion protein (ShaPrP\n                      <jats:sup>sc<\/jats:sup>\n                      ) was 7.02 (0.33)-fold higher in bovine than ovine colon (p&lt;0.0025). In each species, the probe permeabilities decreased according to the formula: P = P\n                      <jats:sub>o<\/jats:sub>\n                      .exp(\u2212K.ra). The \u201cideal\u201d permeabilities, P\n                      <jats:sub>o<\/jats:sub>\n                      are similar, however, K\n                      <jats:sub>(ovine)<\/jats:sub>\n                      = 2.46 (0.20) cm\/h\/nm exceeds K\n                      <jats:sub>(bovine)<\/jats:sub>\n                      = 0.85 (0.15) cm\/h\/nm (p&lt;0.001) indicating that bovine colon has a higher proportion of wide pores than ovine. Image analysis confirmed that HRP permeated through the bovine mucosal layer via a pericryptal paracellular route much more rapidly than in sheep.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS<\/jats:title>\n                    <jats:p>These data may imply that scrapie prion is transmitted in vivo more easily across the low resistance bovine colonic barrier than in other species.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/gut.45.6.879","type":"journal-article","created":{"date-parts":[[2010,6,30]],"date-time":"2010-06-30T12:46:12Z","timestamp":1277901972000},"page":"879-888","source":"Crossref","is-referenced-by-count":16,"title":["Comparison of cattle and sheep colonic permeabilities to horseradish peroxidase and hamster scrapie prion protein in vitro"],"prefix":"10.1136","volume":"45","author":[{"given":"A T","family":"McKie","sequence":"first","affiliation":[{"name":"Division 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