{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T17:19:01Z","timestamp":1767374341442,"version":"3.48.0"},"reference-count":42,"publisher":"BMJ","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"accepted":{"date-parts":[[2000,2,22]]},"published-print":{"date-parts":[[2000,8,1]]},"abstract":"<jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>Luminal anionic sulphide may contribute to epithelial damage in ulcerative colitis. Thiol methyltransferase (TMT) governs sulphide detoxification by the colonic mucosa and circulating erythrocytes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>AIMS<\/jats:title>\n                    <jats:p>To measure levels of TMT activity in erythrocytes of surgically treated cases of colitis or in rectal biopsies of defined groups of colitis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>PATIENTS<\/jats:title>\n                    <jats:p>Venepuncture blood was obtained from 37 blood donors and 27 subjects who had previously undergone a proctocolectomy for colitis: 18 for ulcerative colitis and nine for Crohn's colitis. Rectal biopsies from 122 cases were obtained: 47 without mucosal disease, 33 post-colon resection for cancer, 14 with moderate to severe ulcerative colitis, 15 with quiescent ulcerative colitis, seven with acute Crohn's colitis, and six with radiation proctitis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec sec-type=\"methods\">\n                    <jats:title>METHODS<\/jats:title>\n                    <jats:p>\n                      TMT activity was measured by high performance liquid chromatography with radioactive detection to measure\n                      <jats:sup>14<\/jats:sup>\n                      C methylmercaptoethanol formation, the reaction product of cell extracts incubated with mercaptoethanol and\n                      <jats:sup>14<\/jats:sup>\n                      C S-adenosylmethionine.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>Erythrocyte TMT activity of surgically treated cases of colitis was significantly elevated (p&lt;0.001) compared with control cases. TMT activity of rectal biopsies was significantly decreased (p&lt;0.02) in acute but not quiescent ulcerative colitis, Crohn's colitis, or radiation colitis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS<\/jats:title>\n                    <jats:p>Erythrocyte TMT activity was persistently elevated after proctocolectomy for Crohn's disease and ulcerative colitis. No primary defect of TMT activity was found in any case of unoperated colitis but mucosal activity was diminished with disease progression of ulcerative colitis. Studies of genetic control of TMT activity of erythrocytes in inflammatory bowel disease appear worthwhile.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/gut.47.2.206","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T06:32:32Z","timestamp":1027751552000},"page":"206-210","source":"Crossref","is-referenced-by-count":18,"title":["Thiol methyltransferase activity in inflammatory bowel disease"],"prefix":"10.1136","volume":"47","author":[{"given":"W E","family":"Roediger","sequence":"first","affiliation":[{"name":"Colorectal Unit and Cell Physiology Laboratory of the Department of Surgery, University of Adelaide at the Queen Elizabeth Hospital, Adelaide, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W J","family":"Babidge","sequence":"additional","affiliation":[{"name":"Colorectal Unit and Cell Physiology Laboratory of the Department of Surgery, University of Adelaide at the Queen Elizabeth Hospital, Adelaide, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[2000,8,1]]},"reference":[{"key":"2025121310505138000_47.2.206.1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1097(91)90655-T"},{"key":"2025121310505138000_47.2.206.2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.1998.083_c.x"},{"key":"2025121310505138000_47.2.206.3","first-page":"A170","article-title":"A role for sulfate reducing bacteria in ulcerative colitis.","volume":"98","author":"Florin","year":"1990","journal-title":"Gastroenterology"},{"key":"2025121310505138000_47.2.206.4","first-page":"A15","article-title":"Salicylates inhibit bacterial sulphide production within the colonic lumen in ulcerative colitis.","volume":"37","author":"Pitcher","year":"1995","journal-title":"Gut"},{"key":"2025121310505138000_47.2.206.5","doi-asserted-by":"publisher","DOI":"10.1023\/A:1018848709769"},{"key":"2025121310505138000_47.2.206.6","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(93)91016-B"},{"key":"2025121310505138000_47.2.206.7","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006838231432"},{"key":"2025121310505138000_47.2.206.8","doi-asserted-by":"publisher","DOI":"10.1042\/cs0850623"},{"key":"2025121310505138000_47.2.206.9","first-page":"27","article-title":"5-ASA decreases colonic sulfide formation: Implications for ulcerative colitis.","volume":"24","author":"Roediger","year":"1996","journal-title":"Med Sci Res"},{"key":"2025121310505138000_47.2.206.10","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(98)70311-7"},{"key":"2025121310505138000_47.2.206.11","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(98)70629-8"},{"key":"2025121310505138000_47.2.206.12","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)61120-8"},{"key":"2025121310505138000_47.2.206.13","doi-asserted-by":"publisher","DOI":"10.1136\/gut.38.Suppl_1.A29"},{"key":"2025121310505138000_47.2.206.14","doi-asserted-by":"publisher","DOI":"10.1111\/j.1445-2197.1997.tb01956.x"},{"key":"2025121310505138000_47.2.206.15","first-page":"A664","article-title":"Effect of hydrogen sulphide on cell proliferation of colonic mucosa.","volume":"106","author":"Christl","year":"1994","journal-title":"Gastroenterology"},{"key":"2025121310505138000_47.2.206.16","first-page":"S69","article-title":"Hydrogen sulphide induced damage to the colonic mucosal barrier in the rat.","volume":"33","author":"Aslam","year":"1992","journal-title":"Gut"},{"key":"2025121310505138000_47.2.206.17","doi-asserted-by":"crossref","first-page":"393","DOI":"10.3109\/08923979709046984","article-title":"Sulfide influence on polymorphonuclear functions: a possible role for Ca2+ involvement.","volume":"19","author":"Mariggio","year":"1997","journal-title":"Immunopharmacol Immunotoxicol"},{"key":"2025121310505138000_47.2.206.18","doi-asserted-by":"publisher","DOI":"10.1023\/A:1007034417320"},{"key":"2025121310505138000_47.2.206.19","doi-asserted-by":"publisher","DOI":"10.1136\/gut.41.6.731"},{"key":"2025121310505138000_47.2.206.20","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.10.4.8647346"},{"key":"2025121310505138000_47.2.206.21","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(80)90029-5"},{"key":"2025121310505138000_47.2.206.22","doi-asserted-by":"publisher","DOI":"10.1023\/A:1018867516575"},{"key":"2025121310505138000_47.2.206.23","first-page":"A31","article-title":"Systemic sulphide detoxication in inflammatory bowel disease.","volume":"44","author":"Picton","year":"1999","journal-title":"Gut"},{"key":"2025121310505138000_47.2.206.24","first-page":"A8","article-title":"Is there impaired detoxication of hydrogen sulphide in ulcerative colitis?","volume":"42","author":"Picton","year":"1997","journal-title":"Gut"},{"key":"2025121310505138000_47.2.206.25","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.1136\/bmj.1.4979.1315","article-title":"Biopsy studies in ulcerative colitis.","volume":"i","author":"Truelove","year":"1956","journal-title":"BMJ"},{"key":"2025121310505138000_47.2.206.26","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(78)90331-5"},{"key":"2025121310505138000_47.2.206.27","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3894(92)87011-4"},{"key":"2025121310505138000_47.2.206.28","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.48.7.641"},{"key":"2025121310505138000_47.2.206.29","doi-asserted-by":"crossref","first-page":"671","DOI":"10.3109\/00498259309059404","article-title":"S-methyltransferases in human intestine: differential distribution of the microsomal thiol methyltransferase and cytosolic methyltransferase along the human bowel.","volume":"23","author":"Pacifici","year":"1993","journal-title":"Xenobiotica"},{"key":"2025121310505138000_47.2.206.30","first-page":"A27","article-title":"Mechanisms for detoxifying hydrogen sulphide in the gastrointestinal tract.","volume":"40","author":"Singh","year":"1997","journal-title":"Gut"},{"key":"2025121310505138000_47.2.206.31","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1111\/j.1399-302X.1993.tb00542.x","article-title":"Chemotaxis and degranulation of polymorphonuclear leukocytes in the presence of sulfide.","volume":"8","author":"Persson","year":"1993","journal-title":"Oral Microbiol Immunol"},{"key":"2025121310505138000_47.2.206.32","first-page":"47","article-title":"Dietary habits as risk factors for inflammatory bowel disease.","volume":"7","author":"Tragnone","year":"1995","journal-title":"Eur J Gastroenterol Hepatol"},{"key":"2025121310505138000_47.2.206.33","doi-asserted-by":"publisher","DOI":"10.1136\/gut.41.1.70"},{"key":"2025121310505138000_47.2.206.34","first-page":"A27","article-title":"The contribution of dietary protein from meat to faecal sulphide excretion.","volume":"40","author":"Magee","year":"1997","journal-title":"Gut"},{"key":"2025121310505138000_47.2.206.35","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1038\/clpt.1983.208","article-title":"Thiol methylation pharmacogenetics: heritability of human erythrocyte thiol methyltransferase activity.","volume":"34","author":"Keith","year":"1983","journal-title":"Clin Pharmacol Ther"},{"key":"2025121310505138000_47.2.206.36","doi-asserted-by":"publisher","DOI":"10.1136\/gut.32.1.46"},{"key":"2025121310505138000_47.2.206.37","unstructured":"Roediger WEW (1991) The role of sulphur metabolism and mercapto fatty acids in the aetiology of ulcerative colitis. in Inflammatory bowel disease. Progress in basic research and clinical implications. eds Goebell H Malchow H Ewe K (Kluwer Acad Publications, Lancaster), pp 17\u201327."},{"key":"2025121310505138000_47.2.206.38","doi-asserted-by":"crossref","first-page":"729","DOI":"10.3109\/00365529409092501","article-title":"The expression of glutathione transferase \u03bc in patients with inflammatory bowel disease.","volume":"29","author":"Hertervig","year":"1994","journal-title":"Scand J Gastroenterol"},{"key":"2025121310505138000_47.2.206.39","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2036.1999.00475.x"},{"key":"2025121310505138000_47.2.206.40","doi-asserted-by":"crossref","first-page":"A546","DOI":"10.1016\/0016-5085(95)26496-5","article-title":"Quantification of the essential methyl group donor S-adenosylmethionine in human colorectal mucosa of patients with inflammatory bowel disease (IBD) and colorectal adenomas.","volume":"108","author":"Thiede","year":"1995","journal-title":"Gastroenterology"},{"key":"2025121310505138000_47.2.206.41","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/S0261-5614(06)80012-9","article-title":"Folate status, DNA methylation and colon cancer risk in inflammatory bowel disease.","volume":"14","author":"Cravo","year":"1995","journal-title":"Clin Nutr"},{"key":"2025121310505138000_47.2.206.42","doi-asserted-by":"publisher","DOI":"10.1136\/gut.39.1.1"}],"container-title":["Gut"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/gut.47.2.206","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T18:50:59Z","timestamp":1765651859000},"score":1,"resource":{"primary":{"URL":"https:\/\/gut.bmj.com\/lookup\/doi\/10.1136\/gut.47.2.206"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,8,1]]},"references-count":42,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2000,8,1]]},"published-print":{"date-parts":[[2000,8,1]]}},"alternative-id":["10.1136\/gut.47.2.206"],"URL":"https:\/\/doi.org\/10.1136\/gut.47.2.206","relation":{},"ISSN":["0017-5749","1468-3288"],"issn-type":[{"type":"print","value":"0017-5749"},{"type":"electronic","value":"1468-3288"}],"subject":[],"published":{"date-parts":[[2000,8,1]]}}}