{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T18:46:14Z","timestamp":1788201974167,"version":"build-2803163510"},"reference-count":22,"publisher":"BMJ","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"accepted":{"date-parts":[[2000,8,8]]},"published-print":{"date-parts":[[2001,2,1]]},"abstract":"<jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      In duodenal ulcer patients, intragastric acidity during omeprazole treatment is significantly lower before\n                      <jats:italic>Helicobacter pylori<\/jats:italic>\n                      eradication than after cure.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>AIMS<\/jats:title>\n                    <jats:p>\n                      To determine if\n                      <jats:italic>H pylori<\/jats:italic>\n                      enhances the acid inhibitory potency of omeprazole in isolated parietal cells and on H\n                      <jats:sup>+<\/jats:sup>\n                      \/K\n                      <jats:sup>+<\/jats:sup>\n                      -ATPase.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec sec-type=\"methods\">\n                    <jats:title>METHODS<\/jats:title>\n                    <jats:p>\n                      Rat parietal cells and pig gastric membrane vesicles enriched in H\n                      <jats:sup>+<\/jats:sup>\n                      \/K\n                      <jats:sup>+<\/jats:sup>\n                      -ATPase activity were incubated with\n                      <jats:italic>H pylori<\/jats:italic>\n                      and the\n                      <jats:italic>H pylori<\/jats:italic>\n                      fatty acid\n                      <jats:italic>cis<\/jats:italic>\n                      9,10-methyleneoctadecanoic acid (MOA), and the inhibitory effects of omeprazole on parietal cell acid production, H\n                      <jats:sup>+<\/jats:sup>\n                      \/K\n                      <jats:sup>+<\/jats:sup>\n                      -ATPase enzyme activity, and ATPase mediated proton transport were assessed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      In isolated parietal cells,\n                      <jats:italic>H pylori<\/jats:italic>\n                      and MOA increased the acid inhibitory potency of omeprazole 1.8 fold.\n                      <jats:italic>H pylori<\/jats:italic>\n                      did not affect the inhibitory potency of omeprazole on H\n                      <jats:sup>+<\/jats:sup>\n                      \/K\n                      <jats:sup>+<\/jats:sup>\n                      -ATPase enzyme activity. In proton transport studies,\n                      <jats:italic>H pylori<\/jats:italic>\n                      (intact bacteria and sonicate) and MOA accelerated the onset of the inhibitory effect of omeprazole and enhanced the proton dissipation rate in response to omeprazole.\n                      <jats:italic>H pylori<\/jats:italic>\n                      itself increased proton permeability at the vesicle membrane.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>\n                      Our results show that\n                      <jats:italic>H pylori<\/jats:italic>\n                      augments the acid inhibitory potency of omeprazole in parietal cells and enhances omeprazole induced proton efflux rate from gastric membrane vesicles. We suggest that omeprazole unmasks the permanent effect of\n                      <jats:italic>H pylori<\/jats:italic>\n                      on proton permeability at the apical parietal cell membrane, which is counteracted in the absence of a proton pump inhibitor by a reserve H\n                      <jats:sup>+<\/jats:sup>\n                      \/K\n                      <jats:sup>+<\/jats:sup>\n                      -ATPase capacity.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/gut.48.2.157","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T06:37:59Z","timestamp":1027751879000},"page":"157-162","source":"Crossref","is-referenced-by-count":12,"title":["<i>Helicobacter pylori<\/i>\n                    augments the acid inhibitory effect of omeprazole on parietal cells and gastric H\n                    <sup>+<\/sup>\n                    \/K\n                    <sup>+<\/sup>\n                    -ATPase"],"prefix":"10.1136","volume":"48","author":[{"given":"W","family":"Beil","sequence":"first","affiliation":[{"name":"aDepartment of General Pharmacology, Hannover Medical School, Hannover, Germany, \n\nbDepartment of Gastroenterology and Hepatology, Hannover Medical School, Hannover, Germany, \n\ncDepartment of Physiology, School of Veterinary Medicine, Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K-F","family":"Sewing","sequence":"additional","affiliation":[{"name":"aDepartment of General Pharmacology, Hannover Medical School, Hannover, Germany, \n\nbDepartment of Gastroenterology and Hepatology, Hannover Medical School, Hannover, Germany, \n\ncDepartment of Physiology, School of Veterinary Medicine, Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Busche","sequence":"additional","affiliation":[{"name":"aDepartment of General Pharmacology, Hannover Medical School, Hannover, Germany, \n\nbDepartment of Gastroenterology and Hepatology, Hannover Medical School, Hannover, Germany, \n\ncDepartment of Physiology, School of Veterinary Medicine, Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Wagner","sequence":"additional","affiliation":[{"name":"aDepartment of General Pharmacology, Hannover Medical School, Hannover, Germany, \n\nbDepartment of Gastroenterology and Hepatology, Hannover Medical School, Hannover, Germany, \n\ncDepartment of Physiology, School of Veterinary Medicine, Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2001,2,1]]},"reference":[{"key":"2025121300245415000_48.2.157.1","first-page":"192","article-title":"Ingestion of Campylobacter pyloridis causes gastritis and raised fasting gastric pH.","volume":"82","author":"Morris","year":"1987","journal-title":"Am J 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