{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T10:23:11Z","timestamp":1792146191092,"version":"4.4.0"},"reference-count":30,"publisher":"BMJ","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"accepted":{"date-parts":[[2000,12,5]]},"published-print":{"date-parts":[[2001,5,1]]},"abstract":"<jats:p>Biliary duct carcinomas (BDCs) are relatively rare and the carcinogenic mechanisms underlying their induction are poorly understood. There are two growth patterns, polypoid and non-polypoid infiltrative type, but little information is available concerning the relation between growth pattern and genetic alterations. A comparative study was therefore conducted to clarify if differences in genetic changes, including loss of heterozygosity (LOH) at 5q, 9p, 17p, and 18q, and K-<jats:italic>ras<\/jats:italic> mutations exist between polypoid and non-polypoid infiltrative type BDCs. LOH analysis was performed using microsatellite markers and K-<jats:italic>ras<\/jats:italic> point mutations were analysed by dot blot hybridisation. The incidences of changes for polypoid and non-polypoid infiltrative types were 73% and 26% on 5q, 63% and 59% on 9p, 55% and 50% on 17p, and 20% and 18% on 18q, and 25% and 27% for K-<jats:italic>ras<\/jats:italic> mutations. Most importantly, we found the frequency of 5qLOH to be significantly higher with polypoid growth than in the non-polypoid infiltrative type (p&lt;0.05), especially in extrahepatic duct carcinomas (p&lt;0.05). The incidences of other genetic alterations (LOH at 9p, 17p, and 18q, and K-<jats:italic>ras<\/jats:italic> mutations) showed similar rates with both tumour types. The present data suggest that 5qLOH may have a close relation with polypoid growth in BDCs.<\/jats:p>","DOI":"10.1136\/gut.48.5.656","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T06:37:59Z","timestamp":1027751879000},"page":"656-659","source":"Crossref","is-referenced-by-count":6,"title":["Genetic alterations and growth pattern in biliary duct carcinomas: loss of heterozygosity at chromosome 5q bears a close relation with polypoid growth"],"prefix":"10.1136","volume":"48","author":[{"given":"E","family":"Hidaka","sequence":"first","affiliation":[{"name":"aDepartment of Pathology, Cancer Institute, Tokyo, Japan, \n\nbDepartment of Surgery, Cancer Institute Hospital, Tokyo, Japan, \n\ncDepartment of Surgery, Miyazaki Medical College, Miyazaki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Yanagisawa","sequence":"additional","affiliation":[{"name":"aDepartment of Pathology, Cancer Institute, Tokyo, Japan, \n\nbDepartment of Surgery, Cancer Institute Hospital, Tokyo, Japan, \n\ncDepartment of Surgery, Miyazaki Medical College, Miyazaki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Seki","sequence":"additional","affiliation":[{"name":"aDepartment of Pathology, Cancer Institute, Tokyo, Japan, \n\nbDepartment of Surgery, Cancer Institute Hospital, Tokyo, Japan, \n\ncDepartment of Surgery, Miyazaki Medical College, Miyazaki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Setoguchi","sequence":"additional","affiliation":[{"name":"aDepartment of Pathology, Cancer Institute, Tokyo, Japan, \n\nbDepartment of Surgery, Cancer Institute Hospital, Tokyo, Japan, \n\ncDepartment of Surgery, Miyazaki Medical College, Miyazaki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y","family":"Kato","sequence":"additional","affiliation":[{"name":"aDepartment of Pathology, Cancer Institute, Tokyo, Japan, 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