{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T14:50:31Z","timestamp":1791903031843,"version":"4.3.4"},"reference-count":38,"publisher":"BMJ","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"accepted":{"date-parts":[[2001,2,28]]},"published-print":{"date-parts":[[2001,10,1]]},"abstract":"<jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      Gastric infection with the human pathogen\n                      <jats:italic>Helicobacter pylori<\/jats:italic>\n                      results in a large accumulation of IgA and IgM secreting cells in the gastric mucosa. The molecular mechanisms resulting in B cell migration to the gastric mucosa in\n                      <jats:italic>H pylori<\/jats:italic>\n                      infection are however not known.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>AIMS<\/jats:title>\n                    <jats:p>\n                      To examine expression of the mucosal homing receptor integrin \u03b14\u03b27 and the homing receptor for secondary lymphoid tissues,\n                      <jats:sc>l<\/jats:sc>\n                      -selectin, on lymphocytes activated by gastric, intestinal, or systemic antigens. Furthermore, to examine gastric expression of the mucosal addressin cellular adhesion molecule 1 (MAdCAM-1), the endothelial counter-receptor to integrin \u03b14\u03b27.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>SUBJECTS AND METHODS<\/jats:title>\n                    <jats:p>\n                      H pylori infected individuals were immunised by either gastric (n=8) or intestinal (n=8) delivery of an inactivated cholera vaccine. The resulting circulating vaccine specific B cells were sorted according to \u03b14\u03b27 and\n                      <jats:sc>l<\/jats:sc>\n                      -selectin expression and assayed for production of IgA and IgG using an enzyme linked immunospot assay. In addition, circulating CD4+ T cells from seven\n                      <jats:italic>H pylori<\/jats:italic>\n                      infected individuals were fractionated according to \u03b14\u03b27 and\n                      <jats:sc>l<\/jats:sc>\n                      -selectin expression. The resulting T cell fractions were then assayed for specific proliferation against\n                      <jats:italic>H pylori<\/jats:italic>\n                      or the systemic antigen tetanus toxoid. Finally, gastric expression of MAdCAM-1 was determined by immunohistochemistry in\n                      <jats:italic>H pylori<\/jats:italic>\n                      infected (n=16) and uninfected (n=8) individuals.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Virtually all B cells induced by both gastric and intestinal antigen delivery expressed \u03b14\u03b27 whereas less then half coexpressed\n                      <jats:sc>l<\/jats:sc>\n                      -selectin. Furthermore,\n                      <jats:italic>H pylori<\/jats:italic>\n                      reactive T cells were mainly found in the \u03b14\u03b27+\n                      <jats:sc>l<\/jats:sc>\n                      -selectin+ T cell fraction whereas tetanus specific T cells were largely \u03b14\u03b27\u2212\n                      <jats:sc>l<\/jats:sc>\n                      -selectin+. MAdCAM-1 was present in similar amounts in gastric mucosa from\n                      <jats:italic>H pylori<\/jats:italic>\n                      infected and uninfected individuals.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS<\/jats:title>\n                    <jats:p>B cells and T cells activated by antigens delivered to the gastric mucosa express the mucosal homing receptor integrin \u03b14\u03b27, as do cells activated in the intestine. Together with the observation that gastric endothelial cells express MAdCAM-1, this may partly explain the homing of lymphocytes activated in the stomach or in the small intestine to the gastric mucosa.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/gut.49.4.519","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T06:37:51Z","timestamp":1027751871000},"page":"519-525","source":"Crossref","is-referenced-by-count":42,"title":["Homing commitment of lymphocytes activated in the human gastric and intestinal mucosa"],"prefix":"10.1136","volume":"49","author":[{"given":"M","family":"Quiding-J\u00e4rbrink","sequence":"first","affiliation":[{"name":"aDepartment of Medical Microbiology and Immunology, G\u00f6teborg University, G\u00f6teborg, Sweden, \n\nbDepartment of Surgery, G\u00f6teborg University, G\u00f6teborg, 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