{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T19:19:45Z","timestamp":1765653585485,"version":"3.48.0"},"reference-count":27,"publisher":"BMJ","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"published-print":{"date-parts":[[2002,1]]},"abstract":"\n Background: Different acid and peptic related gastroduodenal diseases are associated with both increased gastric secretion and\n Helicobacter pylori<\/jats:italic>\n infection. Patients with\n H pylori<\/jats:italic>\n associated gastritis or duodenal ulcer have increased serum pepsinogen levels which decrease after eradication. The mechanisms of\n H pylori<\/jats:italic>\n induced gastric mucosal damage are not completely understood.\n <\/jats:p>\n \n Aim: To determine the effects of\n H pylori<\/jats:italic>\n on pepsinogen secretion from isolated human peptic cells.\n <\/jats:p>\n \n Methods: Dispersed human peptic cells were prepared from endoscopically obtained biopsy specimens after collagenase digestion, mechanical disruption, and density gradient centrifugation.\n H pylori<\/jats:italic>\n was obtained from gastric biopsies (antrum and body), and cultured in non-selective and selective media. Isolates of\n H pylori<\/jats:italic>\n were used at different concentrations (1\u201320\u00d710\n 6<\/jats:sup>\n colony forming units (cfu)).\n <\/jats:p>\n \n Results:\n H pylori<\/jats:italic>\n (10\n 6<\/jats:sup>\n \u20132\u00d710\n 7<\/jats:sup>\n cfu) increased basal pepsinogen secretion in a concentration dependent manner. This stimulus was not observed with\n Escherichia coli<\/jats:italic>\n . The increased secretion was in addition to that observed with 0.1 mM histamine and 0.1 mM dibutyryl-cyclic adenosine monophosphate. However,\n H pylori<\/jats:italic>\n did not affect either carbamylcholine (0.1\u201310 \u03bcM) or cholecystokinin (1 \u03bcM) stimulated pepsinogen secretion. Addition of the nitric oxide synthase inhibitor N\n w<\/jats:sup>\n -monomethyl-\n l<\/jats:sc>\n -arginine (1 mM) inhibited\n H pylori<\/jats:italic>\n induced cGMP generation and pepsinogen secretion, which were also reduced in the absence of extracellular calcium.\n H pylori<\/jats:italic>\n induced pepsinogen secretion was not affected by the absence\/presence of the\n cagA<\/jats:italic>\n gene.\n <\/jats:p>\n \n Conclusions:\n H pylori<\/jats:italic>\n increases pepsinogen secretion from human peptic cells through a calcium and nitric oxide mediated intracellular pathway. This effect is independent of the\n H pylori<\/jats:italic>\n virulent\n cagA<\/jats:italic>\n gene, and may be a mechanism of\n H pylori<\/jats:italic>\n induced gastric mucosal damage.\n <\/jats:p>","DOI":"10.1136\/gut.50.1.13","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T06:29:07Z","timestamp":1027751347000},"page":"13-18","source":"Crossref","is-referenced-by-count":52,"title":["Helicobacter pylori<\/i>\n stimulates pepsinogen secretion from isolated human peptic cells"],"prefix":"10.1136","volume":"50","author":[{"given":"S","family":"Lorente","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O","family":"Doiz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M Trinidad","family":"Serrano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Castillo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Lanas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[2002,1,1]]},"reference":[{"key":"2025121311152865000_50.1.13.1","first-page":"109","volume":"2","year":"1987","journal-title":"Lancet"},{"key":"2025121311152865000_50.1.13.2","first-page":"233","volume":"335","year":"1990","journal-title":"Lancet"},{"key":"2025121311152865000_50.1.13.3","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(88)90424-2"},{"key":"2025121311152865000_50.1.13.4","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199302043280503"},{"key":"2025121311152865000_50.1.13.5","doi-asserted-by":"crossref","first-page":"S43","DOI":"10.1016\/S0016-5085(97)80010-8","volume":"113","year":"1997","journal-title":"Gastroenterology"},{"key":"2025121311152865000_50.1.13.6","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/S0016-5085(19)32639-3","volume":"69","year":"1975","journal-title":"Gastroenterology"},{"key":"2025121311152865000_50.1.13.7","first-page":"81","volume":"38","year":"1993","journal-title":"Dig Dis Sci"},{"key":"2025121311152865000_50.1.13.8","first-page":"211","volume":"89","year":"1994","journal-title":"Am J Gastroenterol"},{"key":"2025121311152865000_50.1.13.9","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1097\/00042737-199602000-00012","volume":"8","year":"1996","journal-title":"Eur J Gastroenterol Hepatol"},{"key":"2025121311152865000_50.1.13.10","first-page":"20","volume":"27","year":"1992","journal-title":"Scand J Gastroenterol"},{"key":"2025121311152865000_50.1.13.11","first-page":"9","volume":"181","year":"1991","journal-title":"Scand J Gastroenterol"},{"key":"2025121311152865000_50.1.13.12","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1111\/j.1365-2036.1992.tb00260.x","volume":"6","year":"1992","journal-title":"Aliment Pharmacol Ther"},{"key":"2025121311152865000_50.1.13.13","doi-asserted-by":"crossref","first-page":"678","DOI":"10.3109\/00365529409092493","volume":"29","year":"1994","journal-title":"Scand J Gastroenterol"},{"key":"2025121311152865000_50.1.13.14","doi-asserted-by":"publisher","DOI":"10.1136\/gut.40.1.42"},{"key":"2025121311152865000_50.1.13.15","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1128\/jcm.33.3.752-754.1995","volume":"33","year":"1995","journal-title":"J Clin Microbiol"},{"key":"2025121311152865000_50.1.13.16","doi-asserted-by":"publisher","DOI":"10.1136\/gut.36.5.657"},{"key":"2025121311152865000_50.1.13.17","first-page":"214","volume":"109","year":"1995","journal-title":"Gastroenterology"},{"key":"2025121311152865000_50.1.13.18","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.46.9.826"},{"key":"2025121311152865000_50.1.13.19","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1128\/iai.58.3.603-610.1990","volume":"58","year":"1990","journal-title":"Infect Immun"},{"key":"2025121311152865000_50.1.13.20","doi-asserted-by":"publisher","DOI":"10.1006\/mpat.1998.0225"},{"key":"2025121311152865000_50.1.13.21","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(96)01304-X"},{"key":"2025121311152865000_50.1.13.22","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1056\/NEJM197901113000203","volume":"300","year":"1979","journal-title":"N Engl J Med"},{"key":"2025121311152865000_50.1.13.23","doi-asserted-by":"crossref","first-page":"S54","DOI":"10.1007\/s000110050269","volume":"47","year":"1998","journal-title":"Inflamm Res"},{"key":"2025121311152865000_50.1.13.24","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(80)90404-2"},{"key":"2025121311152865000_50.1.13.25","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(95)90374-7"},{"key":"2025121311152865000_50.1.13.26","first-page":"709","volume":"108","year":"1995","journal-title":"Gastroenterology"},{"key":"2025121311152865000_50.1.13.27","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(96)70062-8"}],"container-title":["Gut"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/gut.50.1.13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T19:15:38Z","timestamp":1765653338000},"score":1,"resource":{"primary":{"URL":"https:\/\/gut.bmj.com\/lookup\/doi\/10.1136\/gut.50.1.13"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,1]]},"references-count":27,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2002,1,1]]},"published-print":{"date-parts":[[2002,1]]}},"alternative-id":["10.1136\/gut.50.1.13"],"URL":"https:\/\/doi.org\/10.1136\/gut.50.1.13","relation":{},"ISSN":["0017-5749","1468-3288"],"issn-type":[{"type":"print","value":"0017-5749"},{"type":"electronic","value":"1468-3288"}],"subject":[],"published":{"date-parts":[[2002,1]]}}}