{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T22:42:29Z","timestamp":1773787349503,"version":"3.50.1"},"reference-count":43,"publisher":"BMJ","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"published-print":{"date-parts":[[2003,11]]},"abstract":"<jats:p>\n                    <jats:bold>Background and aims:<\/jats:bold>\n                    Positron emission tomography (PET) using\n                    <jats:sup>18<\/jats:sup>\n                    F labelled 2-fluoro-2-deoxy-D-glucose (\n                    <jats:sup>18<\/jats:sup>\n                    FDG) is an established imaging tool, although the recent development of a biologically stable thymidine analogue [18F] 3\u2032-deoxy-3-fluorothymidine (\n                    <jats:sup>18<\/jats:sup>\n                    FLT) has allowed PET to image cellular proliferation by utilising the salvage pathway of DNA synthesis. In this study, we have compared uptake of\n                    <jats:sup>18<\/jats:sup>\n                    FLT and\n                    <jats:sup>18<\/jats:sup>\n                    FDG with MIB-1 immunohistochemistry to evaluate the role of PET in quantifying in vivo cellular proliferation in colorectal cancer (CRC).\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Patients and methods:<\/jats:bold>\n                    Patients with resectable, primary, or recurrent CRC were prospectively studied. Thirteen lesions from 10 patients (five males, five females), median age 68 years (range 54\u201387), were evaluated. Patients underwent\n                    <jats:sup>18<\/jats:sup>\n                    FDG and\n                    <jats:sup>18<\/jats:sup>\n                    FLT PET scanning. Tracer uptake within lesions was quantified using standardised uptake values (SUVs). Histopathological examination and MIB-1 immunohistochemistry were performed on all lesions, and proliferation quantified by calculating a labelling index (% of MIB-1 positively stained nuclei within 1500 tumour cells).\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results:<\/jats:bold>\n                    Histology confirmed adenocarcinoma in 12 of 13 lesions; the remaining lesion was reactive. All eight extrahepatic lesions were visualised using both\n                    <jats:sup>18<\/jats:sup>\n                    FLT and\n                    <jats:sup>18<\/jats:sup>\n                    FDG. Three of the five resected liver metastases were also avid for\n                    <jats:sup>18<\/jats:sup>\n                    FLT and showed high proliferation, while the remaining two lesions which demonstrated no uptake of\n                    <jats:sup>18<\/jats:sup>\n                    FLT had correspondingly very low proliferation. There was a statistically significant positive correlation (\n                    <jats:italic>r<\/jats:italic>\n                    =0.8, p&lt;0.01) between SUVs of the tumours visualised with\n                    <jats:sup>18<\/jats:sup>\n                    FLT and the corresponding MIB-1 labelling indices. No such correlation was demonstrated with\n                    <jats:sup>18<\/jats:sup>\n                    FDG avid lesions (\n                    <jats:italic>r<\/jats:italic>\n                    =0.4).\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusions:<\/jats:bold>\n                    <jats:sup>18<\/jats:sup>\n                    FLT PET correlates with cellular proliferation markers in both primary and metastatic CRC. This technique could provide a mechanism for in vivo grading of malignancy and early prediction of response to adjuvant chemotherapy.\n                  <\/jats:p>","DOI":"10.1136\/gut.52.11.1602","type":"journal-article","created":{"date-parts":[[2003,10,21]],"date-time":"2003-10-21T21:43:34Z","timestamp":1066772614000},"page":"1602-1606","source":"Crossref","is-referenced-by-count":105,"title":["In vivo imaging of cellular proliferation in colorectal cancer using positron emission tomography"],"prefix":"10.1136","volume":"52","author":[{"given":"D L","family":"Francis","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Freeman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D","family":"Visvikis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D C","family":"Costa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S K","family":"Luthra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Novelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I","family":"Taylor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P J","family":"Ell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[2003,10,21]]},"reference":[{"key":"2025120523455185000_52.11.1602.1","doi-asserted-by":"crossref","first-page":"1666","DOI":"10.1002\/bjs.1800811136","volume":"81","year":"1994","journal-title":"Br J Surg"},{"key":"2025120523455185000_52.11.1602.2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9610(99)80067-1"},{"key":"2025120523455185000_52.11.1602.3","first-page":"1196","volume":"38","year":"1997","journal-title":"J Nucl Med"},{"key":"2025120523455185000_52.11.1602.4","doi-asserted-by":"publisher","DOI":"10.1001\/archsurg.1996.01430190025007"},{"key":"2025120523455185000_52.11.1602.5","doi-asserted-by":"publisher","DOI":"10.1007\/BF02303744"},{"key":"2025120523455185000_52.11.1602.6","doi-asserted-by":"publisher","DOI":"10.1001\/archsurg.134.5.503"},{"key":"2025120523455185000_52.11.1602.7","doi-asserted-by":"crossref","first-page":"894","DOI":"10.1200\/JCO.1999.17.3.894","volume":"17","year":"1999","journal-title":"J Clin Oncol"},{"key":"2025120523455185000_52.11.1602.8","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9610(00)00406-2"},{"key":"2025120523455185000_52.11.1602.9","doi-asserted-by":"publisher","DOI":"10.1007\/s002590100646"},{"key":"2025120523455185000_52.11.1602.10","doi-asserted-by":"publisher","DOI":"10.1038\/sj.neo.7900075"},{"key":"2025120523455185000_52.11.1602.11","first-page":"615","volume":"59","year":"1999","journal-title":"Cancer Res"},{"key":"2025120523455185000_52.11.1602.12","first-page":"1633","volume":"29","year":"1988","journal-title":"J Nucl Med"},{"key":"2025120523455185000_52.11.1602.13","first-page":"1757","volume":"39","year":"1998","journal-title":"J Nucl Med"},{"key":"2025120523455185000_52.11.1602.14","first-page":"974","volume":"35","year":"1994","journal-title":"J Nucl Med"},{"key":"2025120523455185000_52.11.1602.15","doi-asserted-by":"crossref","first-page":"539","DOI":"10.3109\/02841869209088303","volume":"31","year":"1992","journal-title":"Acta Oncol"},{"key":"2025120523455185000_52.11.1602.16","doi-asserted-by":"crossref","unstructured":"Krohn KA, Mankoff DA, Eary JF. 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