{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T12:27:03Z","timestamp":1790684823129,"version":"4.1.0"},"reference-count":31,"publisher":"BMJ","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Gut"],"published-print":{"date-parts":[[2003,3]]},"abstract":"<jats:p>\n                    <jats:bold>Introduction:<\/jats:bold>\n                    Interleukin 10 knockout (IL-10\u2212\/\u2212) mice spontaneously develop a Th1 T cell mediated colitis with many similarities to Crohn\u2019s disease. Daily injections of IL-10 are unable to induce remission in mice with established disease. In contrast, we have shown previously that intravenous administration of adenoviral vectors encoding IL-10 (AdvmuIL-10) induces hepatic IL-10 release and leads to long term disease suppression with profound systemic immunoregulatory changes.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Aims:<\/jats:bold>\n                    To determine whether rectal delivery of AdvmuIL-10 induces localised colonic IL-10 expression without systemic immune suppression, and assess its therapeutic efficacy in IL-10\u2212\/\u2212 mice with established colitis.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results:<\/jats:bold>\n                    A single rectal infusion of 5\u00d710\n                    <jats:sup>8<\/jats:sup>\n                    PFU AdvmuIL-10 to 10 week IL-10\u2212\/\u2212 mice resulted in a median level of 27.3 pg\/mg IL-10 in colonic homogenates harvested one week later. IL-10\u2212\/\u2212 mice with established colitis treated with an enema of 5\u00d710\n                    <jats:sup>8<\/jats:sup>\n                    PFU AdvmuIL-10 entered clinical and histological remission whereas empty cassette adenovirus (Adv0) or phosphate buffered saline (PBS) treated mice developed progressive disease. After four weeks, the histological score of AdvmuIL-10 treated mice (4.4 (1.5)) was significantly lower than that of Adv0 (11.1 (1.1); p&lt;0.001) and PBS (10.9 (1.0); p&lt;0.01) treated controls. In addition, the stool concentration of IL-1\u03b2 over the four week experiment was significantly higher in mice treated with saline or Adv0 than in those treated with AdvmuIL-10 (p&lt;0.01).\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion:<\/jats:bold>\n                    Local AdvmuIL-10 therapy reverses colitis in IL-10\u2212\/\u2212 mice without the systemic effects seen after intravenous administration. Gene therapy strategies using adenoviral vectors encoding immunoregulatory cytokines may prove to be a potent approach to the treatment of chronic inflammatory diseases such as Crohn\u2019s disease.\n                  <\/jats:p>","DOI":"10.1136\/gut.52.3.363","type":"journal-article","created":{"date-parts":[[2003,2,12]],"date-time":"2003-02-12T20:48:32Z","timestamp":1045082912000},"page":"363-369","source":"Crossref","is-referenced-by-count":31,"title":["Local delivery of adenoviral vectors encoding murine interleukin 10 induces colonic interleukin 10 production and is therapeutic for murine colitis"],"prefix":"10.1136","volume":"52","author":[{"given":"J O","family":"Lindsay","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C 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