{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T23:06:45Z","timestamp":1781564805454,"version":"3.54.5"},"reference-count":41,"publisher":"BMJ","issue":"1","content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["Gut"],"accepted":{"date-parts":[[2012,10,17]]},"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Eosinophilic oesophagitis (EoE) is a chronic inflammatory condition of the oesophagus with limited treatment options. No previous transgenic model has specifically targeted the oesophageal mucosa to induce oesophageal eosinophilia.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Design<\/jats:title>\n                    <jats:p>We developed a mouse model that closely resembles EoE by utilising oxazolone haptenation in mice with transgenic overexpression of an eosinophil poietic and survival factor (interleukin (IL)-5) in resident squamous oesophageal epithelia.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Overexpression of IL-5 in the healthy oesophagus was achieved in transgenic mice (\n                      <jats:italic>L2-IL5<\/jats:italic>\n                      ) using the squamous epithelial promoter Epstein\u2013Barr virus ED-L2. Oxazolone-challenged\n                      <jats:italic>L2-IL5<\/jats:italic>\n                      mice developed dose-dependent pan-oesophageal eosinophilia, including eosinophil microabscess formation and degranulation as well as basal cell hyperplasia. Moreover, oesophagi expressed increased IL-13 and the eosinophil agonist chemokine eotaxin-1. Treatment of these mice with corticosteroids significantly reduced eosinophilia and epithelial inflammation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      <jats:italic>L2-IL5<\/jats:italic>\n                      mice provide a novel experimental model that can potentially be used in preclinical testing of EoE-related therapeutics and mechanistic studies identifying pathogenetic features associated with mucosal eosinophilia.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/gutjnl-2012-303631","type":"journal-article","created":{"date-parts":[[2012,11,17]],"date-time":"2012-11-17T21:23:17Z","timestamp":1353187397000},"page":"43-53","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":52,"title":["Local hypersensitivity reaction in transgenic mice with squamous epithelial IL-5 overexpression provides a novel model of eosinophilic oesophagitis"],"prefix":"10.1136","volume":"63","author":[{"given":"Joanne C","family":"Masterson","sequence":"first","affiliation":[{"name":"Section of Pediatric Gastroenterology, Hepatology and Nutrition, Digestive Health Institute, Aurora, Colorado, USA"},{"name":"Gastrointestinal Eosinophilic Diseases Program, Aurora, Colorado, USA"},{"name":"Children's Hospital Colorado, Aurora, Colorado, USA"},{"name":"Mucosal Inflammation Program, University of Colorado Denver, Aurora, Colorado, USA"},{"name":"University of Colorado Denver School of Medicine, Aurora, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E\u00f3in N","family":"McNamee","sequence":"additional","affiliation":[{"name":"Mucosal Inflammation Program, University of Colorado Denver, Aurora, Colorado, USA"},{"name":"University of Colorado Denver School of Medicine, Aurora, Colorado, USA"},{"name":"Department of Anesthesiology, University of Colorado Denver, Aurora, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lindsay","family":"Hosford","sequence":"additional","affiliation":[{"name":"Section of Pediatric Gastroenterology, Hepatology and Nutrition, Digestive Health Institute, Aurora, Colorado, USA"},{"name":"Gastrointestinal Eosinophilic Diseases Program, Aurora, Colorado, USA"},{"name":"Children's Hospital Colorado, Aurora, Colorado, USA"},{"name":"Mucosal Inflammation Program, University of Colorado Denver, Aurora, Colorado, USA"},{"name":"University of Colorado Denver School of Medicine, Aurora, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelley E","family":"Capocelli","sequence":"additional","affiliation":[{"name":"Gastrointestinal Eosinophilic Diseases Program, Aurora, Colorado, USA"},{"name":"Children's Hospital Colorado, Aurora, Colorado, USA"},{"name":"University of Colorado Denver School of Medicine, Aurora, Colorado, USA"},{"name":"Department of Pathology, Children's Hospital Colorado, Aurora, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Ruybal","sequence":"additional","affiliation":[{"name":"Section of Pediatric Gastroenterology, Hepatology and Nutrition, Digestive Health Institute, Aurora, Colorado, USA"},{"name":"Gastrointestinal Eosinophilic Diseases Program, Aurora, Colorado, USA"},{"name":"Children's Hospital Colorado, Aurora, Colorado, USA"},{"name":"Mucosal Inflammation Program, University of Colorado Denver, Aurora, Colorado, USA"},{"name":"University of Colorado Denver School of Medicine, Aurora, Colorado, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie A","family":"Fillon","sequence":"additional","affiliation":[{"name":"Section of Pediatric Gastroenterology, Hepatology and Nutrition, Digestive Health Institute, Aurora, Colorado, USA"},{"name":"Gastrointestinal Eosinophilic Diseases Program, Aurora, 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