{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T12:26:55Z","timestamp":1793276815520,"version":"4.5.0"},"reference-count":33,"publisher":"BMJ","issue":"12","content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["Gut"],"accepted":{"date-parts":[[2012,10,30]]},"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Non-alcoholic fatty liver disease (NAFLD) is prevalent among obese people and is considered the hepatic manifestation of metabolic syndrome. However, not all obese individuals develop NAFLD. Our objective was to demonstrate the role of the gut microbiota in NAFLD development using transplantation experiments in mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Design<\/jats:title>\n                    <jats:p>Two donor C57BL\/6J mice were selected on the basis of their responses to a high-fat diet (HFD). Although both mice displayed similar body weight gain, one mouse, called the \u2018responder\u2019, developed hyperglycaemia and had a high plasma concentration of pro-inflammatory cytokines. The other, called a \u2018non-responder\u2019, was normoglycaemic and had a lower level of systemic inflammation. Germ-free mice were colonised with intestinal microbiota from either the responder or the non-responder and then fed the same HFD.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Mice that received microbiota from different donors developed comparable obesity on the HFD. The responder-receiver (RR) group developed fasting hyperglycaemia and insulinaemia, whereas the non-responder-receiver (NRR) group remained normoglycaemic. In contrast to NRR mice, RR mice developed hepatic macrovesicular steatosis, which was confirmed by a higher liver concentration of triglycerides and increased expression of genes involved in de-novo lipogenesis. Pyrosequencing of the 16S ribosomal RNA genes revealed that RR and NRR mice had distinct gut microbiota including differences at the phylum, genera and species levels.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Differences in microbiota composition can determine response to a HFD in mice. These results further demonstrate that the gut microbiota contributes to the development of NAFLD independently of obesity.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/gutjnl-2012-303816","type":"journal-article","created":{"date-parts":[[2012,11,29]],"date-time":"2012-11-29T22:11:19Z","timestamp":1354227079000},"page":"1787-1794","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":888,"title":["Intestinal microbiota determines development of non-alcoholic fatty liver disease in mice"],"prefix":"10.1136","volume":"62","author":[{"given":"Tiphaine","family":"Le Roy","sequence":"first","affiliation":[{"name":"INRA, UMR1319 Micalis, Jouy-en-Josas, France"},{"name":"AgroParisTech, UMR Micalis, Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Llopis","sequence":"additional","affiliation":[{"name":"INRA, UMR1319 Micalis, Jouy-en-Josas, France"},{"name":"AgroParisTech, UMR Micalis, Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Lepage","sequence":"additional","affiliation":[{"name":"INRA, UMR1319 Micalis, Jouy-en-Josas, France"},{"name":"AgroParisTech, UMR Micalis, Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aur\u00e9lia","family":"Bruneau","sequence":"additional","affiliation":[{"name":"INRA, UMR1319 Micalis, Jouy-en-Josas, France"},{"name":"AgroParisTech, UMR Micalis, Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvie","family":"Rabot","sequence":"additional","affiliation":[{"name":"INRA, UMR1319 Micalis, Jouy-en-Josas, France"},{"name":"AgroParisTech, UMR Micalis, Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Bevilacqua","sequence":"additional","affiliation":[{"name":"INRA, UMR1313 GABI, Plateforme de Microg\u00e9nomique expressionnelle \u201cIso Cell Express\u201d (ICE), Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice","family":"Martin","sequence":"additional","affiliation":[{"name":"INRA, UMR1313 GABI, Plateforme de Microg\u00e9nomique expressionnelle \u201cIso Cell Express\u201d (ICE), Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Philippe","sequence":"additional","affiliation":[{"name":"INRA, UMR1319 Micalis, Jouy-en-Josas, France"},{"name":"AgroParisTech, UMR Micalis, Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francine","family":"Walker","sequence":"additional","affiliation":[{"name":"INSERM U773, UFR de M\u00e9decine Paris Diderot, Universit\u00e9 Paris Diderot, Sorbonne Paris Cit\u00e9, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9","family":"Bado","sequence":"additional","affiliation":[{"name":"INSERM U773, UFR de M\u00e9decine Paris Diderot, Universit\u00e9 Paris Diderot, Sorbonne Paris Cit\u00e9, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Perlemuter","sequence":"additional","affiliation":[{"name":"INSERM, U996, IPSIT, Clamart, France"},{"name":"Facult\u00e9 de m\u00e9decine Paris-Sud, Universit\u00e9 Paris-Sud, Kremlin-Bic\u00eatre, France"},{"name":"AP-HP, H\u00f4pital Antoine B\u00e9cl\u00e8re, Service d'h\u00e9pato-gastroent\u00e9rologie, Clamart, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne-Marie","family":"Cassard-Doulcier","sequence":"additional","affiliation":[{"name":"INSERM, U996, IPSIT, Clamart, France"},{"name":"Facult\u00e9 de m\u00e9decine Paris-Sud, Universit\u00e9 Paris-Sud, Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"G\u00e9rard","sequence":"additional","affiliation":[{"name":"INRA, UMR1319 Micalis, Jouy-en-Josas, France"},{"name":"AgroParisTech, UMR Micalis, Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2012,11,29]]},"reference":[{"key":"2025090712171864000_62.12.1787.1","doi-asserted-by":"publisher","DOI":"10.2174\/138161210791208875"},{"key":"2025090712171864000_62.12.1787.2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-nutr-072610-145146"},{"key":"2025090712171864000_62.12.1787.3","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2010.172"},{"key":"2025090712171864000_62.12.1787.4","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0407076101"},{"key":"2025090712171864000_62.12.1787.5","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0605374104"},{"key":"2025090712171864000_62.12.1787.6","doi-asserted-by":"publisher","DOI":"10.1096\/fj.10-164921"},{"key":"2025090712171864000_62.12.1787.7","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2010.11.049"},{"key":"2025090712171864000_62.12.1787.8","doi-asserted-by":"publisher","DOI":"10.1038\/nature10809"},{"key":"2025090712171864000_62.12.1787.9","doi-asserted-by":"publisher","DOI":"10.1538\/expanim.52.99"},{"key":"2025090712171864000_62.12.1787.10","doi-asserted-by":"publisher","DOI":"10.1021\/pr800352k"},{"key":"2025090712171864000_62.12.1787.11","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2011-301012"},{"key":"2025090712171864000_62.12.1787.12","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1978"},{"key":"2025090712171864000_62.12.1787.13","doi-asserted-by":"crossref","first-page":"3854","DOI":"10.1128\/AEM.64.10.3854-3859.1998","article-title":"Temperature gradient gel electrophoresis analysis of 16S rRNA from human fecal samples reveals stable and host-specific communities of active bacteria","volume":"64","author":"Zoetendal","year":"1998","journal-title":"Appl Environ Microbiol"},{"key":"2025090712171864000_62.12.1787.14","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114507433001"},{"key":"2025090712171864000_62.12.1787.15","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/S0021-9258(18)64849-5","article-title":"A simple method for the isolation and purification of total lipides from animal tissues","volume":"226","author":"Folch","year":"1957","journal-title":"J Biol Chem"},{"key":"2025090712171864000_62.12.1787.16","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90021-2"},{"key":"2025090712171864000_62.12.1787.17","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn879"},{"key":"2025090712171864000_62.12.1787.18","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.f.303"},{"key":"2025090712171864000_62.12.1787.19","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btl158"},{"key":"2025090712171864000_62.12.1787.20","doi-asserted-by":"publisher","DOI":"10.1016\/j.plipres.2008.08.001"},{"key":"2025090712171864000_62.12.1787.21","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0001668"},{"key":"2025090712171864000_62.12.1787.22","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-010-9582-9"},{"key":"2025090712171864000_62.12.1787.23","doi-asserted-by":"crossref","first-page":"1701","DOI":"10.1136\/gutjnl-2011-301689","article-title":"Gut-derived lipopolysaccharide augments adipose macrophage accumulation but is not essential for impaired glucose or insulin tolerance in mice","volume":"21","author":"Caesar","year":"2012","journal-title":"Gut"},{"key":"2025090712171864000_62.12.1787.24","doi-asserted-by":"publisher","DOI":"10.1096\/fj.07-102723"},{"key":"2025090712171864000_62.12.1787.25","doi-asserted-by":"crossref","unstructured":"Shindo N Fujisawa T Sugimoto K . Involvement of microsomal triglyceride transfer protein in nonalcoholic steatohepatitis in novel spontaneous mouse model. J Hepatol 52:903\u201312.","DOI":"10.1016\/j.jhep.2009.12.033"},{"key":"2025090712171864000_62.12.1787.26","doi-asserted-by":"publisher","DOI":"10.1038\/nature05414"},{"key":"2025090712171864000_62.12.1787.27","doi-asserted-by":"publisher","DOI":"10.3920\/BM2010.0028"},{"key":"2025090712171864000_62.12.1787.28","doi-asserted-by":"publisher","DOI":"10.5740\/jaoacint.SGE_Macfarlane"},{"key":"2025090712171864000_62.12.1787.29","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114509992807"},{"key":"2025090712171864000_62.12.1787.30","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3000322"},{"key":"2025090712171864000_62.12.1787.31","doi-asserted-by":"publisher","DOI":"10.1016\/S0278-6915(02)00324-1"},{"key":"2025090712171864000_62.12.1787.32","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-6496(03)00285-X"},{"key":"2025090712171864000_62.12.1787.33","doi-asserted-by":"publisher","DOI":"10.1097\/MCG.0b013e3181dadb10"}],"container-title":["Gut"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/gutjnl-2012-303816","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,7]],"date-time":"2025-09-07T19:17:24Z","timestamp":1757272644000},"score":1,"resource":{"primary":{"URL":"https:\/\/gut.bmj.com\/lookup\/doi\/10.1136\/gutjnl-2012-303816"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,11,29]]},"references-count":33,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2013,11,7]]},"published-print":{"date-parts":[[2013,12]]}},"alternative-id":["10.1136\/gutjnl-2012-303816"],"URL":"https:\/\/doi.org\/10.1136\/gutjnl-2012-303816","relation":{},"ISSN":["0017-5749","1468-3288"],"issn-type":[{"value":"0017-5749","type":"print"},{"value":"1468-3288","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,11,29]]}}}