{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T17:17:57Z","timestamp":1793812677778,"version":"4.6.1"},"reference-count":45,"publisher":"BMJ","issue":"12","content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["Gut"],"accepted":{"date-parts":[[2017,4,17]]},"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      Postoperative ileus (POI) is assumed to result from myeloid cells infiltrating the intestinal\n                      <jats:italic>muscularis externa<\/jats:italic>\n                      (ME) in patients undergoing abdominal surgery. In the current study, we investigated the role of infiltrating monocytes in a murine model of intestinal manipulation (IM)-induced POI in order to clarify whether monocytes mediate tissue damage and intestinal dysfunction or they are rather involved in the recovery of gastrointestinal (GI) motility.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Design<\/jats:title>\n                    <jats:p>\n                      IM was performed in mice with defective monocyte migration to tissues (C-C motif chemokine receptor 2,\n                      <jats:italic>\n                        Ccr2\n                        <jats:sup>\n                          \u2212\/\n                          <jats:italic>\n                            <jats:sup>\u2212<\/jats:sup>\n                          <\/jats:italic>\n                        <\/jats:sup>\n                      <\/jats:italic>\n                      mice) and wild-type (WT) mice to study the role of monocytes and monocyte-derived macrophages (M\u03a6s) during onset and resolution of ME inflammation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      At early time points, IM-induced GI transit delay and inflammation were equal in WT and\n                      <jats:italic>\n                        Ccr2\n                        <jats:italic>\n                          <jats:sup>\u2212<\/jats:sup>\n                        <\/jats:italic>\n                        <jats:sup>\/<\/jats:sup>\n                      <\/jats:italic>\n                      <jats:sup>\u2212<\/jats:sup>\n                      mice. However, GI transit recovery after IM was significantly delayed in\n                      <jats:italic>\n                        Ccr2\n                        <jats:italic>\n                          <jats:sup>\u2212<\/jats:sup>\n                        <\/jats:italic>\n                        <jats:sup>\/<\/jats:sup>\n                        <jats:italic>\n                          <jats:sup>\u2212<\/jats:sup>\n                        <\/jats:italic>\n                      <\/jats:italic>\n                      mice compared with WT mice, associated with increased neutrophil-mediated immunopathology and persistent impaired neuromuscular function. During recovery, monocyte-derived M\u03a6s acquire pro-resolving features that aided in the resolution of inflammation. In line, bone marrow reconstitution and treatment with M\u03a6 colony-stimulating factor 1 enhanced monocyte recruitment and M\u03a6 differentiation and ameliorated GI transit in\n                      <jats:italic>\n                        Ccr2\n                        <jats:italic>\n                          <jats:sup>\u2212<\/jats:sup>\n                        <\/jats:italic>\n                        <jats:sup>\/<\/jats:sup>\n                        <jats:italic>\n                          <jats:sup>\u2212<\/jats:sup>\n                        <\/jats:italic>\n                      <\/jats:italic>\n                      mice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our study reveals a critical role for monocyte-derived M\u03a6s in restoring intestinal homeostasis after surgical trauma. From a therapeutic point of view, our data indicate that inappropriate targeting of monocytes may increase neutrophil-mediated immunopathology and prolong the clinical outcome of POI, while future therapies should be aimed at enhancing M\u03a6 physiological repair functions.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/gutjnl-2016-313144","type":"journal-article","created":{"date-parts":[[2017,6,14]],"date-time":"2017-06-14T20:55:16Z","timestamp":1497473716000},"page":"2098-2109","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":85,"title":["CCR2-dependent monocyte-derived macrophages resolve inflammation and restore gut motility in postoperative ileus"],"prefix":"10.1136","volume":"66","author":[{"given":"Giovanna","family":"Farro","sequence":"first","affiliation":[{"name":"Translational Research Center for Gastrointestinal Disorders (TARGID), Department of Clinical and Experimental Medicine, KU Leuven, Leuven, 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Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gera","family":"Goverse","sequence":"additional","affiliation":[{"name":"Translational Research Center for Gastrointestinal Disorders (TARGID), Department of Clinical and Experimental Medicine, KU Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina Di","family":"Giovangiulio","sequence":"additional","affiliation":[{"name":"Translational Research Center for Gastrointestinal Disorders (TARGID), Department of Clinical and Experimental Medicine, KU Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Stakenborg","sequence":"additional","affiliation":[{"name":"Translational Research Center for Gastrointestinal Disorders (TARGID), Department of Clinical and Experimental Medicine, KU Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisa","family":"Meroni","sequence":"additional","affiliation":[{"name":"Translational Research Center for Gastrointestinal Disorders (TARGID), Department of Clinical and Experimental Medicine, KU Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"D\u2019Errico","sequence":"additional","affiliation":[{"name":"Translational Research Center for Gastrointestinal Disorders (TARGID), Department of Clinical and Experimental Medicine, KU Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvon","family":"Elkrim","sequence":"additional","affiliation":[{"name":"Lab of Cellular and Molecular Immunology, Vrije Universiteit Brussel, Brussels, Belgium"},{"name":"Myeloid Cell Immunology Lab, VIB Inflammation Research Center, Ghent, 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