{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T12:20:53Z","timestamp":1791807653317,"version":"4.3.4"},"reference-count":56,"publisher":"BMJ","issue":"2","content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["Gut"],"accepted":{"date-parts":[[2017,2,20]]},"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To investigate the beneficial role of prebiotics on endothelial dysfunction, an early key marker of cardiovascular diseases, in an original mouse model linking steatosis and endothelial dysfunction.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Design<\/jats:title>\n                    <jats:p>\n                      We examined the contribution of the gut microbiota to vascular dysfunction observed in apolipoprotein E knockout (Apoe\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      ) mice fed an n-3 polyunsaturated fatty acid (PUFA)-depleted diet for 12\u2005weeks with or without inulin-type fructans (ITFs) supplementation for the last 15\u2005days. Mesenteric and carotid arteries were isolated to evaluate endothelium-dependent relaxation ex vivo. Caecal microbiota composition (Illumina Sequencing of the 16S rRNA gene) and key pathways\/mediators involved in the control of vascular function, including bile acid (BA) profiling, gut and liver key gene expression, nitric oxide and gut hormones production were also assessed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      ITF supplementation totally reverses endothelial dysfunction in mesenteric and carotid arteries of n-3 PUFA-depleted Apoe\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      mice via activation of the nitric oxide (NO) synthase\/NO pathway. Gut microbiota changes induced by prebiotic treatment consist in increased NO-producing bacteria, replenishment of abundance in\n                      <jats:italic>Akkermansia<\/jats:italic>\n                      and decreased abundance in bacterial taxa involved in secondary BA synthesis. Changes in gut and liver gene expression also occur upon ITFs suggesting increased glucagon-like peptide 1 production and BA turnover as drivers of endothelium function preservation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>We demonstrate for the first time that ITF improve endothelial dysfunction, implicating a short-term adaptation of both gut microbiota and key gut peptides. If confirmed in humans, prebiotics could be proposed as a novel approach in the prevention of metabolic disorders-related cardiovascular diseases.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/gutjnl-2016-313316","type":"journal-article","created":{"date-parts":[[2017,4,4]],"date-time":"2017-04-04T20:44:27Z","timestamp":1491338667000},"page":"271-283","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":184,"title":["Targeting the gut microbiota with inulin-type fructans: preclinical demonstration of a novel approach in the management of endothelial dysfunction"],"prefix":"10.1136","volume":"67","author":[{"given":"Emilie","family":"Catry","sequence":"first","affiliation":[{"name":"Metabolism and Nutrition Research Group, Louvain Drug Research Institute, Universit\u00e9 catholique de Louvain, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laure B","family":"Bindels","sequence":"additional","affiliation":[{"name":"Metabolism and Nutrition Research Group, Louvain Drug Research Institute, Universit\u00e9 catholique de Louvain, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Tailleux","sequence":"additional","affiliation":[{"name":"European Genomic Institute for Diabetes (EGID), Univ Lille, Lille, France"},{"name":"INSERM UMR 1011, Lille, France"},{"name":"Institut Pasteur de Lille, Lille, France"},{"name":"CHU de Lille, Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Lestavel","sequence":"additional","affiliation":[{"name":"European Genomic Institute for Diabetes (EGID), Univ Lille, Lille, France"},{"name":"INSERM UMR 1011, Lille, France"},{"name":"Institut Pasteur de Lille, Lille, France"},{"name":"CHU de Lille, Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Audrey M","family":"Neyrinck","sequence":"additional","affiliation":[{"name":"Metabolism and Nutrition Research Group, Louvain Drug Research Institute, Universit\u00e9 catholique de Louvain, Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Fran\u00e7ois","family":"Goossens","sequence":"additional","affiliation":[{"name":"Centre Universitaire de Mesures et d'Analyses, Univ. 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