{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,12]],"date-time":"2026-11-12T09:09:16Z","timestamp":1794474556420,"version":"4.9.1"},"reference-count":51,"publisher":"BMJ","issue":"6","content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["Gut"],"accepted":{"date-parts":[[2017,6,9]]},"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>We aimed to characterise the microbial changes associated with histological stages of gastric tumourigenesis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Design<\/jats:title>\n                    <jats:p>We performed 16S rRNA gene analysis of gastric mucosal samples from 81 cases including superficial gastritis (SG), atrophic gastritis (AG), intestinal metaplasia (IM) and gastric cancer (GC) from Xi\u2019an, China, to determine mucosal microbiome dysbiosis across stages of GC. We validated the results in mucosal samples of 126 cases from Inner Mongolia, China.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      We observed significant mucosa microbial dysbiosis in IM and GC subjects, with significant enrichment of 21 and depletion of 10 bacterial taxa in GC compared with SG (q&lt;0.05). Microbial network analysis showed increasing correlation strengths among them with disease progression (p&lt;0.001). Five GC-enriched bacterial taxa whose species identifications correspond to\n                      <jats:italic>Peptostreptococcus stomatis<\/jats:italic>\n                      ,\n                      <jats:italic>Streptococcus anginosus<\/jats:italic>\n                      ,\n                      <jats:italic>Parvimonas micra<\/jats:italic>\n                      ,\n                      <jats:italic>Slackia exigua<\/jats:italic>\n                      and\n                      <jats:italic>Dialister pneumosintes<\/jats:italic>\n                      had significant centralities in the GC ecological network (p&lt;0.05) and classified GC from SG with an area under the receiver-operating curve (AUC) of 0.82. Moreover, stronger interactions among gastric microbes were observed in\n                      <jats:italic>Helicobacter pylori<\/jats:italic>\n                      -negative samples compared with\n                      <jats:italic>H. pylori<\/jats:italic>\n                      -positive samples in SG and IM. The fold changes of selected bacteria, and strengths of their interactions were successfully validated in the Inner Mongolian cohort, in which the five bacterial markers distinguished GC from SG with an AUC of 0.81.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      In addition to microbial compositional changes, we identified differences in bacterial interactions across stages of gastric carcinogenesis. The significant enrichments and network centralities suggest potentially important roles of\n                      <jats:italic>P. stomatis<\/jats:italic>\n                      ,\n                      <jats:italic>D. pneumosintes<\/jats:italic>\n                      ,\n                      <jats:italic>S. exigua<\/jats:italic>\n                      ,\n                      <jats:italic>P. micra<\/jats:italic>\n                      and\n                      <jats:italic>S. anginosus<\/jats:italic>\n                      in GC progression.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/gutjnl-2017-314281","type":"journal-article","created":{"date-parts":[[2017,8,1]],"date-time":"2017-08-01T20:50:29Z","timestamp":1501620629000},"page":"1024-1032","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":652,"title":["Mucosal microbiome dysbiosis in gastric carcinogenesis"],"prefix":"10.1136","volume":"67","author":[{"given":"Olabisi Oluwabukola","family":"Coker","sequence":"first","affiliation":[{"name":"Department of Medicine and Therapeutics, Institute of Digestive Disease, State Key Laboratory of Digestive Disease, Li Ka Shing Institute of Health Sciences, CUHK Shenzhen Research Institute, The Chinese University of Hong Kong, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenwei","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of Medicine and Therapeutics, Institute of Digestive Disease, State Key Laboratory of Digestive Disease, Li Ka Shing Institute of Health Sciences, CUHK Shenzhen Research Institute, The Chinese University of Hong Kong, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongzhan","family":"Nie","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cancer Biology, Xijing Hospital, Fourth Military Medical University, Xian, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guijun","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, Inner Mongolia People\u2019s Hospital, Hohhot, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Medicine and Therapeutics, Institute of Digestive Disease, State Key Laboratory of Digestive Disease, Li Ka Shing Institute of Health Sciences, CUHK Shenzhen Research Institute, The Chinese University of Hong Kong, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geicho","family":"Nakatsu","sequence":"additional","affiliation":[{"name":"Department of Medicine and Therapeutics, Institute of Digestive Disease, State Key Laboratory of Digestive Disease, Li Ka Shing Institute of Health Sciences, CUHK Shenzhen Research Institute, The Chinese University of Hong Kong, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William KK","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Medicine and Therapeutics, Institute of Digestive Disease, State Key Laboratory of Digestive Disease, Li Ka Shing Institute of Health Sciences, CUHK Shenzhen Research Institute, The Chinese University of Hong Kong, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunny Hei","family":"Wong","sequence":"additional","affiliation":[{"name":"Department of Medicine and Therapeutics, Institute of Digestive Disease, State Key Laboratory of Digestive Disease, Li Ka Shing Institute of Health Sciences, CUHK Shenzhen Research Institute, The Chinese University of Hong Kong, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zigui","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph J Y","family":"Sung","sequence":"additional","affiliation":[{"name":"Department of Medicine and Therapeutics, Institute of Digestive Disease, State Key Laboratory of Digestive Disease, Li Ka Shing Institute of Health Sciences, CUHK Shenzhen Research Institute, The Chinese University of Hong Kong, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Medicine and Therapeutics, Institute of Digestive Disease, State Key Laboratory of Digestive Disease, Li Ka Shing Institute of Health Sciences, CUHK Shenzhen Research Institute, The Chinese University of Hong Kong, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2017,8,1]]},"reference":[{"key":"2025120619132744000_67.6.1024.1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21332"},{"key":"2025120619132744000_67.6.1024.2","first-page":"6735","article-title":"Human gastric carcinogenesis: a multistep and multifactorial process--first American Cancer society award lecture on Cancer epidemiology and Prevention","volume":"52","author":"Correa","year":"1992","journal-title":"Cancer Res"},{"key":"2025120619132744000_67.6.1024.3","first-page":"302","article-title":"Risk factors in gastric Cancer","volume":"14","author":"Compare","year":"2010","journal-title":"Eur Rev Med Pharmacol Sci"},{"key":"2025120619132744000_67.6.1024.4","doi-asserted-by":"publisher","DOI":"10.1177\/2050640614566846"},{"key":"2025120619132744000_67.6.1024.5","doi-asserted-by":"publisher","DOI":"10.1038\/srep04202"},{"key":"2025120619132744000_67.6.1024.6","doi-asserted-by":"publisher","DOI":"10.1111\/hel.12145"},{"key":"2025120619132744000_67.6.1024.7","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.007302-0"},{"key":"2025120619132744000_67.6.1024.8","doi-asserted-by":"publisher","DOI":"10.1002\/path.1868"},{"key":"2025120619132744000_67.6.1024.9","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2010.09.048"},{"key":"2025120619132744000_67.6.1024.10","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-08-3856"},{"key":"2025120619132744000_67.6.1024.11","first-page":"100444","article-title":"Cross-sectional analysis of Helicobacter pylori infection in physical examination population","volume":"7","author":"Zheng","year":"2013","journal-title":"Chinese journal of clinical medicine"},{"key":"2025120619132744000_67.6.1024.12","first-page":"760","article-title":"Analysis of infestation rate of Helicobacter pylori and factors affcecting the infestation in inner Mongolia border recruits","volume":"39","author":"Jw","year":"2014","journal-title":"Med J Chn PLA"},{"key":"2025120619132744000_67.6.1024.13","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01541-09"},{"key":"2025120619132744000_67.6.1024.14","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts252"},{"key":"2025120619132744000_67.6.1024.15","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr381"},{"key":"2025120619132744000_67.6.1024.16","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1111\/hel.12214","article-title":"An Appropriate Cutoff Value for determining the colonization of Helicobacter pylori by the Pyrosequencing Method: Comparison with Conventional Methods","volume":"20","author":"Kim","year":"2015","journal-title":"Helicobacter"},{"key":"2025120619132744000_67.6.1024.17","doi-asserted-by":"publisher","DOI":"10.1198\/jasa.2011.tm10563"},{"key":"2025120619132744000_67.6.1024.18","doi-asserted-by":"publisher","DOI":"10.1214\/14-AOAS771"},{"key":"2025120619132744000_67.6.1024.19","doi-asserted-by":"publisher","DOI":"10.1198\/004017005000000319"},{"key":"2025120619132744000_67.6.1024.20","doi-asserted-by":"publisher","DOI":"10.1198\/016214506000000843"},{"key":"2025120619132744000_67.6.1024.21","doi-asserted-by":"crossref","unstructured":"Chen T , Wh Y , Izard J , et al . The Human Oral Microbiome Database: a web Accessible resource for investigating Oral microbe Taxonomic and Genomic information. Database;2010;2010. baq013.","DOI":"10.1093\/database\/baq013"},{"key":"2025120619132744000_67.6.1024.22","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pcbi.1002687","article-title":"Inferring correlation networks from genomic survey data","volume":"8","author":"Friedman","year":"2012","journal-title":"PLoS Comput Biol"},{"key":"2025120619132744000_67.6.1024.23","doi-asserted-by":"publisher","DOI":"10.1186\/1745-6150-9-12"},{"key":"2025120619132744000_67.6.1024.24","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2676"},{"key":"2025120619132744000_67.6.1024.25","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2014.64"},{"key":"2025120619132744000_67.6.1024.26","doi-asserted-by":"crossref","first-page":"404","DOI":"10.4172\/2161-069X.1000404","article-title":"Oral Bacteria and bowel diseases \u2013 Mini Review","volume":"06","author":"Yoneda","year":"2016","journal-title":"J Gastrointest Dig Syst"},{"key":"2025120619132744000_67.6.1024.27","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgt249"},{"key":"2025120619132744000_67.6.1024.28","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms9727"},{"key":"2025120619132744000_67.6.1024.29","doi-asserted-by":"crossref","first-page":"S34","DOI":"10.1097\/MCG.0000000000000233","article-title":"Preliminary results on clinical effects of probiotic Lactobacillus salivarius LS01 in children affected by atopic dermatitis","volume":"48 Suppl 1","author":"Niccoli","year":"2014","journal-title":"J Clin Gastroenterol"},{"key":"2025120619132744000_67.6.1024.30","doi-asserted-by":"crossref","first-page":"8113","DOI":"10.1007\/s00253-015-6679-x","article-title":"Probiotic attributes of Lactobacillus fermentum isolated from human feces and dairy products","volume":"99","author":"Archer","year":"2015","journal-title":"Appl Microbiol Biotechnol"},{"key":"2025120619132744000_67.6.1024.31","doi-asserted-by":"crossref","first-page":"1395","DOI":"10.1016\/j.celrep.2016.01.017","article-title":"Gastric Helicobacter pylori infection affects local and distant microbial populations and Host responses","volume":"14","author":"Kienesberger","year":"2016","journal-title":"Cell Rep"},{"key":"2025120619132744000_67.6.1024.32","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0506655103"},{"key":"2025120619132744000_67.6.1024.33","doi-asserted-by":"crossref","first-page":"26191","DOI":"10.3402\/mehd.v26.26191","article-title":"Dysbiosis of the gut Microbiota in disease","volume":"26","author":"Carding","year":"2015","journal-title":"Microbial Ecology in Health & Disease"},{"key":"2025120619132744000_67.6.1024.34","doi-asserted-by":"publisher","DOI":"10.1038\/srep18594"},{"key":"2025120619132744000_67.6.1024.35","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0154090","article-title":"Intestinal Microbiota is Influenced by gender and body Mass Index","volume":"11","author":"Haro","year":"2016","journal-title":"PLoS One"},{"key":"2025120619132744000_67.6.1024.36","doi-asserted-by":"publisher","DOI":"10.3390\/nu7010017"},{"key":"2025120619132744000_67.6.1024.37","first-page":"2991","article-title":"Presence of Streptococcus anginosus DNA in esophageal Cancer, dysplasia of esophagus, and gastric Cancer","volume":"58","author":"Sasaki","year":"1998","journal-title":"Cancer Res"},{"key":"2025120619132744000_67.6.1024.38","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2003.tb01471.x"},{"key":"2025120619132744000_67.6.1024.39","doi-asserted-by":"publisher","DOI":"10.1111\/j.2041-1014.2010.00599.x"},{"key":"2025120619132744000_67.6.1024.40","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bdj.2016.865"},{"key":"2025120619132744000_67.6.1024.41","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0149653","article-title":"Association between Tooth loss and gastric Cancer: a Meta-Analysis of Observational Studies","volume":"11","author":"Yin","year":"2016","journal-title":"PLoS One"},{"key":"2025120619132744000_67.6.1024.42","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2016-312580"},{"key":"2025120619132744000_67.6.1024.43","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1406199111"},{"key":"2025120619132744000_67.6.1024.44","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2015.04.011"},{"key":"2025120619132744000_67.6.1024.45","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2001.27999"},{"key":"2025120619132744000_67.6.1024.46","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2016-312904"},{"key":"2025120619132744000_67.6.1024.47","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-12-1600"},{"key":"2025120619132744000_67.6.1024.48","doi-asserted-by":"publisher","DOI":"10.1097\/00004836-200603000-00015"},{"key":"2025120619132744000_67.6.1024.49","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.04.051"},{"key":"2025120619132744000_67.6.1024.50","doi-asserted-by":"publisher","DOI":"10.1093\/femsec\/fiw161"},{"key":"2025120619132744000_67.6.1024.51","doi-asserted-by":"publisher","DOI":"10.1038\/nature11465"}],"container-title":["Gut"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/gutjnl-2017-314281","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,7]],"date-time":"2025-12-07T03:13:36Z","timestamp":1765077216000},"score":1,"resource":{"primary":{"URL":"https:\/\/gut.bmj.com\/lookup\/doi\/10.1136\/gutjnl-2017-314281"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,1]]},"references-count":51,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2018,5,8]]},"published-print":{"date-parts":[[2018,6]]}},"alternative-id":["10.1136\/gutjnl-2017-314281"],"URL":"https:\/\/doi.org\/10.1136\/gutjnl-2017-314281","relation":{"has-part":[{"id-type":"doi","id":"https:\/\/doi.org\/10.1136\/gutjnl-2017-314281.supp1","asserted-by":"subject"},{"id-type":"doi","id":"https:\/\/doi.org\/10.1136\/gutjnl-2017-314281.supp50","asserted-by":"subject"},{"id-type":"doi","id":"https:\/\/doi.org\/10.1136\/gutjnl-2017-314281.supp2","asserted-by":"subject"},{"id-type":"doi","id":"https:\/\/doi.org\/10.1136\/gutjnl-2017-314281.supp4","asserted-by":"subject"},{"id-type":"doi","id":"https:\/\/doi.org\/10.1136\/gutjnl-2017-314281.supp5","asserted-by":"subject"},{"id-type":"doi","id":"https:\/\/doi.org\/10.1136\/gutjnl-2017-314281.supp8","asserted-by":"subject"},{"id-type":"doi","id":"https:\/\/doi.org\/10.1136\/gutjnl-2017-314281.supp9","asserted-by":"subject"},{"id-type":"doi","id":"https:\/\/doi.org\/10.1136\/gutjnl-2017-314281.supp10","asserted-by":"subject"},{"id-type":"doi","id":"https:\/\/doi.org\/10.1136\/gutjnl-2017-314281.supp25","asserted-by":"subject"},{"id-type":"doi","id":"https:\/\/doi.org\/10.1136\/gutjnl-2017-314281.supp6","asserted-by":"subject"},{"id-type":"doi","id":"https:\/\/doi.org\/10.1136\/gutjnl-2017-314281.supp11","asserted-by":"subject"},{"id-type":"doi","id":"https:\/\/doi.org\/10.1136\/gutjnl-2017-314281.supp15","asserted-by":"subject"},{"id-type":"doi","id":"https:\/\/doi.org\/10.1136\/gutjnl-2017-314281.supp16","asserted-by":"subject"},{"id-type":"doi","id":"https:\/\/doi.org\/10.1136\/gutjnl-2017-314281.supp17","asserted-by":"subject"},{"id-type":"doi","id":"https:\/\/doi.org\/10.1136\/gutjnl-2017-314281.supp18","asserted-by":"subject"},{"id-type":"doi","id":"https:\/\/doi.org\/10.1136\/gutjnl-2017-314281.supp24","asserted-by":"subject"}]},"ISSN":["0017-5749","1468-3288"],"issn-type":[{"value":"0017-5749","type":"print"},{"value":"1468-3288","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,1]]}}}