{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T09:33:31Z","timestamp":1773308011530,"version":"3.50.1"},"reference-count":33,"publisher":"BMJ","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Heart"],"published-print":{"date-parts":[[2008,3]]},"abstract":"<jats:sec>\n                  <jats:title>Objectives:<\/jats:title>\n                  <jats:p>To clarify the role of granzyme B in acute coronary syndrome.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Design and Setting:<\/jats:title>\n                  <jats:p>Granzyme B is a member of the serine esterase family released from cytotoxic lymphocytes and plays an important role in cellular apoptosis by activating intracellular caspases. Granzyme B expression was compared between patients with stable and unstable angina pectoris (UAP).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Patients:<\/jats:title>\n                  <jats:p>173 patients with coronary artery disease (CAD) were enrolled. 84 patients were found to have stable angina pectoris (SAP) and 89 patients to have UAP.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods:<\/jats:title>\n                  <jats:p>Peripheral blood was drawn from the patients. Peripheral blood mononuclear cells (PBMCs) isolated by gradient centrifugation were cultured at a density of 2\u00d7106 cells\/ml for 24 hours. The supernatants were collected 24 hours after incubation and the granzyme B level was measured by enzyme-linked immunosorbent assay. Polychromic flow cytometric analysis was performed to evaluate the expression of granzyme B in the cells.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results:<\/jats:title>\n                  <jats:p>Granzyme B production from PBMCs of UAP patients was significantly higher than from those of patients with SAP (39.1 (SEM 6.6) versus 17.0 (SEM 1.8) pg\/ml, p&lt;0.05). Granzyme B production from PBMCs increased with the increasing TIMI risk score in UAP patients. The percentage of granzyme B-positive lymphocytes to CD3-positive lymphocytes in UAP patients was significantly higher than in SAP (32.1% (SEM 1.6%) versus 18.4% (SEM 0.9%), p&lt;0.01).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions:<\/jats:title>\n                  <jats:p>These results suggest that granzyme B might play an important role in triggering acute coronary events by inducing apoptosis and the degradation of atherosclerotic coronary plaques.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1136\/hrt.2006.110023","type":"journal-article","created":{"date-parts":[[2007,6,25]],"date-time":"2007-06-25T20:34:37Z","timestamp":1182803677000},"page":"305-310","source":"Crossref","is-referenced-by-count":22,"title":["Increased granzyme B production from peripheral blood mononuclear cells in patients with acute coronary syndrome"],"prefix":"10.1136","volume":"94","author":[{"given":"R","family":"Tsuru","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"Kondo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y","family":"Hojo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Gama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O","family":"Mizuno","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Katsuki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Shimada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Kikuchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Yashiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[2007,6,25]]},"reference":[{"key":"2025092004255842000_94.3.305.1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(89)90953-7"},{"key":"2025092004255842000_94.3.305.2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.87.4.1179"},{"key":"2025092004255842000_94.3.305.3","doi-asserted-by":"publisher","DOI":"10.1136\/hrt.69.5.377"},{"key":"2025092004255842000_94.3.305.4","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.90.2.775"},{"key":"2025092004255842000_94.3.305.5","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9150(91)90235-U"},{"key":"2025092004255842000_94.3.305.6","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.89.1.36"},{"key":"2025092004255842000_94.3.305.7","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.90.4.1669"},{"key":"2025092004255842000_94.3.305.8","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.16.8.1070"},{"key":"2025092004255842000_94.3.305.9","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117619"},{"key":"2025092004255842000_94.3.305.10","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000090690.67322.51"},{"key":"2025092004255842000_94.3.305.11","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000042674.89762.20"},{"key":"2025092004255842000_94.3.305.12","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64036-9"},{"key":"2025092004255842000_94.3.305.13","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9150(96)06077-7"},{"key":"2025092004255842000_94.3.305.14","doi-asserted-by":"publisher","DOI":"10.1038\/377446a0"},{"key":"2025092004255842000_94.3.305.15","doi-asserted-by":"publisher","DOI":"10.1084\/jem.192.10.1391"},{"key":"2025092004255842000_94.3.305.16","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.11.3781-3794.2000"},{"key":"2025092004255842000_94.3.305.17","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.43.30468"},{"key":"2025092004255842000_94.3.305.18","doi-asserted-by":"crossref","first-page":"7161","DOI":"10.4049\/jimmunol.151.12.7161","article-title":"Human granzyme B degrades aggrecan proteoglycan in matrix synthesized by chondrocytes.","volume":"151","author":"Froelich","year":"1993","journal-title":"J Immunol"},{"key":"2025092004255842000_94.3.305.19","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M412001200"},{"key":"2025092004255842000_94.3.305.20","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(97)00109-0"},{"key":"2025092004255842000_94.3.305.21","unstructured":"Cannon CP Braunwald E . Unstable Angina. In: Braunwald E Zeipes DP Libby P , eds. Heart Disease Philadelphia, Pennsylvania:W.B. Saunders Company, 2001:1232\u201371."},{"key":"2025092004255842000_94.3.305.22","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000108926.04022.0C"},{"key":"2025092004255842000_94.3.305.23","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1177\/000456329803500607","article-title":"Analytical evaluation of particle-enhanced immunonephelometric assays for C-reactive protein, serum amyloid A and mannose-binding protein in human serum.","volume":"35","author":"Ledue","year":"1998","journal-title":"Ann Clin Biochem"},{"key":"2025092004255842000_94.3.305.24","doi-asserted-by":"publisher","DOI":"10.1001\/jama.284.7.835"},{"key":"2025092004255842000_94.3.305.25","doi-asserted-by":"publisher","DOI":"10.1161\/hc0502.103348"},{"key":"2025092004255842000_94.3.305.26","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000147162.51930.b7"},{"key":"2025092004255842000_94.3.305.27","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000149753.74793.88"},{"key":"2025092004255842000_94.3.305.28","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M412001200"},{"key":"2025092004255842000_94.3.305.29","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2004.04.019"},{"key":"2025092004255842000_94.3.305.30","doi-asserted-by":"publisher","DOI":"10.1161\/hc2601.091703"},{"key":"2025092004255842000_94.3.305.31","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.atherosclerosis.2004.09.011","article-title":"Cytomegalovirus infection is associated with elevated interleukin-10 in coronary artery disease.","volume":"179","author":"Sun","year":"2005","journal-title":"Atherosclerosis"},{"key":"2025092004255842000_94.3.305.32","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.98.9.845"},{"key":"2025092004255842000_94.3.305.33","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1016\/0167-4889(91)90207-E","article-title":"Tumor necrosis factor (TNF) inhibits interleukin (IL)-1 and\/or IL-6 stimulated synthesis of C-reactive protein (CRP) and serum amyloid A (SAA) in primary cultures of human hepatocytes.","volume":"1091","author":"Yap","year":"1991","journal-title":"Biochim Biophys Acta"}],"container-title":["Heart"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/hrt.2006.110023","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T11:26:03Z","timestamp":1758367563000},"score":1,"resource":{"primary":{"URL":"https:\/\/heart.bmj.com\/lookup\/doi\/10.1136\/hrt.2006.110023"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,6,25]]},"references-count":33,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2008,2,14]]},"published-print":{"date-parts":[[2008,3]]}},"alternative-id":["10.1136\/hrt.2006.110023"],"URL":"https:\/\/doi.org\/10.1136\/hrt.2006.110023","relation":{},"ISSN":["1355-6037","1468-201X"],"issn-type":[{"value":"1355-6037","type":"print"},{"value":"1468-201X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,6,25]]}}}