{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T16:28:03Z","timestamp":1776702483656,"version":"3.51.2"},"reference-count":33,"publisher":"BMJ","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Heart"],"accepted":{"date-parts":[[2010,9,28]]},"published-print":{"date-parts":[[2011,1]]},"abstract":"<jats:sec>\n                  <jats:title>Objective<\/jats:title>\n                  <jats:p>Patients with cardiorenal syndrome (CRS) have high cardiovascular morbidity. Endothelial progenitor cells (EPC) constitute an endogenous vascular repairsystem, protecting against atherosclerosis development. Erythropoietin (EPO) treatment may have beneficial effects by mobilizing EPC from the bonemarrow. Our objective is to determine EPC levels and effects of EPO therapy on EPC levels in CRS patients.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Design<\/jats:title>\n                  <jats:p>Open-label randomized trial.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Setting<\/jats:title>\n                  <jats:p>Part of the EPOCARES-trial, conducted in Utrecht (Netherlands).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Patients<\/jats:title>\n                  <jats:p>Patients with CRS and anaemia and healthy controls were included.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Interventions<\/jats:title>\n                  <jats:p>Patients were randomized to receive EPO therapy (50\u2005IU\/kg\/wk) for 52\u2005weeks or no EPO therapy.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Main outcome measures<\/jats:title>\n                  <jats:p>CD34<jats:sup>+<\/jats:sup>KDR<jats:sup>+<\/jats:sup>-EPC, cultured EPC outgrowth and function at baseline, after 18\u2005days and after 52\u2005weeks.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Patients showed lower CD34<jats:sup>+<\/jats:sup>KDR<jats:sup>+<\/jats:sup>-cell numbers compared to controls (6(12) vs. 19(19) cells\/10<jats:sup>5<\/jats:sup> granulocytes; p=0.010), despite increased levels of stromal cell-derived factor-1\u03b1; (3.1(0.8) vs 2.6(0.3) ng\/ml; p=0.001). EPC outgrowth and function were not different between patients and controls. EPC levels did not change after 18\u2005days with or without EPO treatment. CD34<jats:sup>+<\/jats:sup>KDR<jats:sup>+<\/jats:sup>-cells significantly declined after 52\u2005weeks in the non-treated group (p=0.028). Long-term EPO therapy did not significantly affect this reduction in CD34<jats:sup>+<\/jats:sup>KDR<jats:sup>+<\/jats:sup>-EPC levels.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>CRS patients showed reduced CD34<jats:sup>+<\/jats:sup>KDR<jats:sup>+<\/jats:sup>-EPC levels compared to controls, consistent with a reduced vascular regenerative potential and despite upregulated SDF-1\u03b1 levels. Over a one-year follow-up period a marked 68% further reduction in EPC levels was observed in the patient group without EPO treatment. In spite of promising experimental studies, our longitudinal, randomized study did not show significant influence of either short- or long-term EPO therapy on reduced EPC levels in CRS patients.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1136\/hrt.2010.194654","type":"journal-article","created":{"date-parts":[[2010,11,11]],"date-time":"2010-11-11T21:04:25Z","timestamp":1289509465000},"page":"60-65","source":"Crossref","is-referenced-by-count":16,"title":["Short- and long-term effects of erythropoietin treatment on endothelial progenitor cell levels in patients with cardiorenal syndrome"],"prefix":"10.1136","volume":"97","clinical-trial-number":[{"clinical-trial-number":"nct00356733","registry":"10.18810\/clinical-trials-gov"}],"author":[{"given":"Kim E","family":"Jie","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karien","family":"van der 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