{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T20:45:04Z","timestamp":1788209104427,"version":"build-2803163510"},"reference-count":28,"publisher":"BMJ","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"accepted":{"date-parts":[[2012,1,7]]},"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:sec>\n                  <jats:title>Aims<\/jats:title>\n                  <jats:p>Accurate serum aldosterone determination is critical to the screening and diagnosis of primary aldosteronism, the localisation of aldosterone producing tumours, and the investigation of other disorders of the renin-angiotensin system. Mass spectrometry offers a means to overcome problems with method-dependent bias between competitive immunoassays for aldosterone. The authors have developed a simple, sensitive and precise liquid\u2013liquid extraction aldosterone method for the ABSCIEX API-5000 liquid chromatography and tandem mass spectrometry (LC-MS\/MS) system.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Using d7-aldosterone internal standard, 500\u2005\u03bcl of sample is extracted with 2500\u2005\u03bcl of methyl tertbutyl ether followed by dry-down, reconstitution and LC-MS\/MS analysis in ESI negative mode. Method validation was undertaken using standard approaches and comparison made against a commercial radioimmunoassay. Accuracy was assessed using EQA material with assigned aldosterone concentrations.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The assay was linear up to 3420\u2005pmol\/l (LOQ=50\u2005pmol\/l, LOD&lt;22\u2005pmol\/l). Total CVs were \u22645% for concentrations \u2265120\u2005pmol\/l and 10% at the LOQ. Mean accuracy was 98.5% against GCMS assigned material.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>The authors present a precise, sensitive and simple aldosterone method suitable for routine clinical use that requires no solid phase extraction or specialised ion sources.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1136\/jclinpath-2011-200564","type":"journal-article","created":{"date-parts":[[2012,3,12]],"date-time":"2012-03-12T20:42:26Z","timestamp":1331584946000},"page":"457-462","source":"Crossref","is-referenced-by-count":51,"title":["Determination of serum aldosterone by liquid chromatography and tandem mass spectrometry: a liquid\u2013liquid extraction method for the ABSCIEX API-5000 mass spectrometry system"],"prefix":"10.1136","volume":"65","author":[{"given":"Jessica Grace","family":"Van Der Gugten","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua","family":"Dubland","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua-Fen","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Joseph","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel T","family":"Holmes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2012,3,12]]},"reference":[{"key":"2025092505164719000_65.5.457.1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2008-0104"},{"key":"2025092505164719000_65.5.457.2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0029-1246186"},{"key":"2025092505164719000_65.5.457.3","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2010-0457"},{"key":"2025092505164719000_65.5.457.4","unstructured":"Gardner D Shoback D . 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