{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T11:20:43Z","timestamp":1790421643455,"version":"4.1.0"},"reference-count":44,"publisher":"BMJ","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"accepted":{"date-parts":[[2012,5,9]]},"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:sec>\n                  <jats:title>Aim<\/jats:title>\n                  <jats:p>To determine whether intestinal <jats:italic>Staphylococcus spp.<\/jats:italic> and their pathogenic features differed between coeliac disease (CD) patients and healthy controls.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>60 children, including active CD (n=20) and non-active CD (n=20) patients and healthy controls (n=20), were studied. Staphylococci were isolated from faeces and identified by PCR and DNA sequencing. The carriage of virulent genes, including adhesion (<jats:italic>atlE<\/jats:italic> and <jats:italic>fbe<\/jats:italic>), cell aggregation (<jats:italic>icaD<\/jats:italic>), global regulatory (<jats:italic>agr<\/jats:italic> and <jats:italic>sar<\/jats:italic>) and methicillin-resistant (<jats:italic>mecA<\/jats:italic>) genes, was analysed by PCR.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>\n                     <jats:italic>Staphylococcus<\/jats:italic> \n                     <jats:italic>epidermidis<\/jats:italic> was more abundant in the microbiota of active and non-active CD patients than in controls. <jats:italic>Staphylococcus<\/jats:italic> \n                     <jats:italic>haemolyticus<\/jats:italic> was more abundant in active CD patients than in control subjects. <jats:italic>Staphylococcus<\/jats:italic> \n                     <jats:italic>aureus<\/jats:italic> was less abundant in active CD patients than in the other child groups. <jats:italic>Staphylococcus spp.<\/jats:italic> diversity was higher in active CD patients than in non-active CD patients and controls. The presence of the <jats:italic>mecA<\/jats:italic> gene and the simultaneous presence of both the <jats:italic>mecA<\/jats:italic> and <jats:italic>atlE<\/jats:italic> genes were higher in <jats:italic>S. epidermidis<\/jats:italic> clones isolated from CD patients, with active and non-active disease, than in those from control subjects. The individual presence of the other virulent genes was lower in <jats:italic>S. epidermidis<\/jats:italic> from active CD patients than in those from the other -child- groups.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Increased abundance of <jats:italic>S. epidermidis<\/jats:italic> carrying the <jats:italic>mecA<\/jats:italic> gene, in active and non-active CD patients, most likely reflects increased exposure of these subjects to opportunistic pathogens and antimicrobials.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1136\/jclinpath-2012-200759","type":"journal-article","created":{"date-parts":[[2012,6,21]],"date-time":"2012-06-21T09:21:18Z","timestamp":1340270478000},"page":"830-834","source":"Crossref","is-referenced-by-count":55,"title":["Intestinal <i>Staphylococcus spp.<\/i> and virulent features associated with coeliac 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