{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T05:28:16Z","timestamp":1791350896460,"version":"4.3.1"},"reference-count":25,"publisher":"BMJ","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"accepted":{"date-parts":[[2012,5,8]]},"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:p>Plasma cell myeloma (PCM) exhibits immunophenotypic aberrancies that can be used for minimal residual disease (MRD) detection after therapy. The authors sought to determine whether non-neoplastic plasma cells, especially in the bone marrow (BM) post various therapies, would exhibit immunophenotypic variations interfering PCM MRD detection. The authors studied the flow cytometric immunophenotypes of non-neoplastic plasma cells from 50 BM specimens, including 12 untreated BM and 38 BM specimens from patients with non-plasmacytic haematological malignancies undergoing various therapies, and compared with 59 BM specimens positive for PCM MRD. Non-neoplastic plasma cells showed heterogeneous expressions of CD45 (78% (41\u2013100)) and CD19 (80% (52\u201397)), and were negative for CD20 and CD117. CD56 was observed in a small subset (6% (0\u201337)) and CD28 in a larger subset (15% (0\u201359)) of non-neoplastic plasma cells, with the latter more frequently expressed in post-treatment BMs (p=0.01). However, despite a partial immunophenotypic overlap, PCM cells could be reliably discriminated from non-neoplastic plasma cells by the presence of a higher number of aberrancies (3 (1\u20136) vs 0 (0\u20132)) and stronger intensity and uniformity of aberrant expression (p&lt;0.001 in each marker using a cut-off value). In addition, simultaneous assessment of cytoplasmic \u03ba\/\u03bb with surface markers detected light chain restriction in all 59 PCM cases. In conclusion, non-neoplastic plasma cells in BM are more immunophenotypically heterogeneous than previously understood; however, these immunophenotypic variations differ from those of PCM. With advances in multicolour flow cytometry and application of recently validated markers, PCM MRD may still be reliably distinguished from non-neoplastic plasma cells.<\/jats:p>","DOI":"10.1136\/jclinpath-2012-200881","type":"journal-article","created":{"date-parts":[[2012,6,8]],"date-time":"2012-06-08T23:29:16Z","timestamp":1339198156000},"page":"823-829","source":"Crossref","is-referenced-by-count":48,"title":["Immunophenotypic heterogeneity of normal plasma cells: comparison with minimal residual plasma cell myeloma"],"prefix":"10.1136","volume":"65","author":[{"given":"Dingsheng","family":"Liu","sequence":"first","affiliation":[{"name":"1Department of Hematopathology, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA"},{"name":"2Department of Hematology, No.1 Hospital of Huaian, Nanjing Medical University, Huaian, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei","family":"Lin","sequence":"additional","affiliation":[{"name":"1Department of Hematopathology, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Hu","sequence":"additional","affiliation":[{"name":"1Department of Hematopathology, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Zhou","sequence":"additional","affiliation":[{"name":"1Department of Hematopathology, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guilin","family":"Tang","sequence":"additional","affiliation":[{"name":"1Department of Hematopathology, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda","family":"Powers","sequence":"additional","affiliation":[{"name":"1Department of Hematopathology, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L Jeffrey","family":"Medeiros","sequence":"additional","affiliation":[{"name":"1Department of Hematopathology, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey L","family":"Jorgensen","sequence":"additional","affiliation":[{"name":"1Department of Hematopathology, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sa A","family":"Wang","sequence":"additional","affiliation":[{"name":"1Department of Hematopathology, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2012,6,8]]},"reference":[{"key":"2025120519325527000_65.9.823.1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.11080"},{"key":"2025120519325527000_65.9.823.2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-05-159624"},{"key":"2025120519325527000_65.9.823.3","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2007.15.4120"},{"key":"2025120519325527000_65.9.823.4","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2010.33.1967"},{"key":"2025120519325527000_65.9.823.5","unstructured":"Paiva B Gutierrez NC Rosinol L . 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