{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T20:52:44Z","timestamp":1793393564420,"version":"4.5.0"},"reference-count":21,"publisher":"BMJ","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"accepted":{"date-parts":[[2012,7,26]]},"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>Infection following transplantation is a cause of morbidity and mortality. Perfusion fluid (PF) used to preserve organs between recovery and transplantation represents a medium suitable for the growth of microbes. We evaluated the relevance of positive growth from PF sampled before the implantation of kidney or kidney\u2013pancreas (KP) allografts.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Between January 2007 and January 2011, 548 kidney\/KP transplants were performed in our centre. A retrospective review of patient records with culture-positive PF was performed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      PF was received from 483 (88%) patients, of which 35 (7%, 95% CI 5.3% to 9.9%) were positive for bacteria (31\/483, 6.4%, 95% CI 4.6% to 9.8%) and fungi (4\/483, 0.8%, 95% CI 0.3% to 2.1%). Thirty-two of the 35 culture-positive PF (91.4%, 95% CI 77.6% to 97%) were considered insignificant. The remaining three patients developed sepsis postoperatively, which was considered to be possibly related to growth in PF;\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                      in one and\n                      <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                      in two. Of the non-skin flora bacteria cultured from PF, six were resistant to the prophylactic antibiotic given intraoperatively, but only one developed infection postoperatively (\n                      <jats:italic>E coli<\/jats:italic>\n                      , resistant to the co-amoxiclav).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Significant attributable morbidity associated with PF-positive culture results was relatively rare. Culture of organisms other than Enterobacteriaceae or fungi are likely to represent contamination.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/jclinpath-2012-200918","type":"journal-article","created":{"date-parts":[[2012,8,23]],"date-time":"2012-08-23T20:25:28Z","timestamp":1345753528000},"page":"1132-1135","source":"Crossref","is-referenced-by-count":11,"title":["Low predictive value of positive transplant perfusion fluid cultures for diagnosing postoperative infections in kidney and kidney\u2013pancreas transplantation"],"prefix":"10.1136","volume":"65","author":[{"given":"Meaghan P","family":"Cotter","sequence":"first","affiliation":[{"name":"Department of Microbiology, Beaumont Hospital, Dublin, Ireland"},{"name":"Department of Clinical Microbiology, The Royal College of Surgeons in Ireland, Dublin, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Smyth","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, The Royal College of Surgeons in Ireland, Dublin, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanne","family":"O'Gorman","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Beaumont Hospital, Dublin, Ireland"},{"name":"Department of Clinical Microbiology, The Royal College of Surgeons in Ireland, Dublin, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Browne","sequence":"additional","affiliation":[{"name":"Division of Nephrology, University of British Columbia, Vancouver, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David P","family":"Hickey","sequence":"additional","affiliation":[{"name":"Department of Urology and Transplantation, Beaumont Hospital, Dublin, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hilary","family":"Humphreys","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Beaumont Hospital, Dublin, Ireland"},{"name":"Department of Clinical Microbiology, The Royal College of Surgeons in Ireland, Dublin, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2012,8,22]]},"reference":[{"key":"2025120518570210000_65.12.1132.1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-198403000-00020"},{"key":"2025120518570210000_65.12.1132.2","doi-asserted-by":"publisher","DOI":"10.1001\/archsurg.1978.01370150041006"},{"key":"2025120518570210000_65.12.1132.3","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/S0140-6736(75)90960-5","article-title":"Staphylococcus aureus transmitted in transplanted kidneys","volume":"2","author":"Doig","year":"1975","journal-title":"Lancet"},{"key":"2025120518570210000_65.12.1132.4","doi-asserted-by":"publisher","DOI":"10.1177\/153857447901300310"},{"key":"2025120518570210000_65.12.1132.5","first-page":"49","article-title":"Infections after transplantation of a contaminated kidney","volume":"92","author":"Bijnen","year":"1985","journal-title":"Scand J Urol Nephrol Suppl"},{"key":"2025120518570210000_65.12.1132.6","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2011.01.019"},{"key":"2025120518570210000_65.12.1132.7","doi-asserted-by":"publisher","DOI":"10.1086\/595688"},{"key":"2025120518570210000_65.12.1132.8","doi-asserted-by":"publisher","DOI":"10.1016\/0196-6553(88)90053-3"},{"key":"2025120518570210000_65.12.1132.9","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-197512000-00004"},{"key":"2025120518570210000_65.12.1132.10","doi-asserted-by":"publisher","DOI":"10.1016\/j.transproceed.2005.08.053"},{"key":"2025120518570210000_65.12.1132.11","doi-asserted-by":"crossref","first-page":"pii:20165","DOI":"10.2807\/ese.17.18.20165-en","article-title":"Possible contamination of organ preservation fluid with Bacillus cereus: the United Kingdom response","volume":"17","author":"Brown","year":"2012","journal-title":"Euro Surveill"},{"key":"2025120518570210000_65.12.1132.12","unstructured":"Benedetti E Sileri P . Donor selection and management. In: Gruessner R Sutherland D , eds. Transplantation of the pancreas. New York: Springer-Verlag, 2004:113\u201315."},{"key":"2025120518570210000_65.12.1132.13","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.1800690819"},{"key":"2025120518570210000_65.12.1132.14","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2010.02.008"},{"key":"2025120518570210000_65.12.1132.15","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-2277.2005.00140.x"},{"key":"2025120518570210000_65.12.1132.16","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfn622"},{"key":"2025120518570210000_65.12.1132.17","doi-asserted-by":"publisher","DOI":"10.1007\/s00147-004-0792-6"},{"key":"2025120518570210000_65.12.1132.18","first-page":"895","article-title":"Incidence of transport medium contamination in cadaver kidney procurement","volume":"20","author":"Benoit","year":"1988","journal-title":"Transplant Proc"},{"key":"2025120518570210000_65.12.1132.19","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9610(05)80611-7"},{"key":"2025120518570210000_65.12.1132.20","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-197901000-00016"},{"key":"2025120518570210000_65.12.1132.21","doi-asserted-by":"publisher","DOI":"10.1097\/01.TP.0000086468.40268.F9"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jclinpath-2012-200918","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T02:57:09Z","timestamp":1764989829000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jclinpath-2012-200918"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,8,22]]},"references-count":21,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2012,11,16]]},"published-print":{"date-parts":[[2012,12]]}},"alternative-id":["10.1136\/jclinpath-2012-200918"],"URL":"https:\/\/doi.org\/10.1136\/jclinpath-2012-200918","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"value":"0021-9746","type":"print"},{"value":"1472-4146","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,8,22]]}}}