{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T01:15:51Z","timestamp":1780362951935,"version":"3.54.1"},"reference-count":9,"publisher":"BMJ","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"accepted":{"date-parts":[[2012,12,10]]},"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>In primary aldosteronism (PA) the main source of aldosterone hypersecretion is an aldosterone-producing adenoma (APA) or a bilateral hyperplasia. Histopathology of the adrenal gland from patients with PA has been difficult, as there are no morphological criteria to ascertain which are the cells that produce aldosterone. We therefore applied new specific antibodies to explore which cells in the adrenal contain the enzymes for aldosterone and cortisol production, respectively.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Adrenals from 24 patients with PA were studied. After routine preparation, consecutive sections were stained with antibodies for CYP11B1 (cortisol) and CYP11B2 (aldosterone) enzymes.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>APA had a strong immunoreactivity for CYB11B2. In adrenals from seven patients, we found no APA, but several nodules with strong CYB11B2 immunoreactivity, indicating aldosterone-producing nodular hyperplasia.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Immunohistochemistry of adrenal steroidogenic enzymes provides novel diagnostic information. This may become an important part of routine histopathology, and contribute to improved clinical management in PA.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1136\/jclinpath-2012-201287","type":"journal-article","created":{"date-parts":[[2013,2,23]],"date-time":"2013-02-23T07:43:09Z","timestamp":1361605389000},"page":"351-354","source":"Crossref","is-referenced-by-count":28,"title":["Immunohistochemistry improves histopathologic diagnosis in primary aldosteronism"],"prefix":"10.1136","volume":"66","author":[{"given":"Cristina","family":"Volpe","sequence":"first","affiliation":[{"name":"Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden"},{"name":"Division of Endocrinology, Department of Internal Medicine, S\u00f6dersjukhuset, Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anders","family":"H\u00f6\u00f6g","sequence":"additional","affiliation":[{"name":"Department of Oncology and Pathology, Karolinska Institutet, Stockholm, Sweden"},{"name":"Department of Clinical Pathology and Cytology, Karolinska University Hospital Solna, Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tadashi","family":"Ogishima","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Sciences, Kyushu University, Fukuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuniaki","family":"Mukai","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, School of Medicine, Keio University 35 Shinanomachi, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marja","family":"Thor\u00e9n","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden"},{"name":"Department of Endocrinology, Metabolism and Diabetes, Karolinska University Hospital Solna, Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bertil","family":"Hamberger","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden"},{"name":"Department of Breast and Endocrine Surgery, Karolinska University Hospital Solna, Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2013,2,22]]},"reference":[{"key":"2025092505024324000_66.4.351.1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0031-1295438"},{"key":"2025092505024324000_66.4.351.2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2005-0681"},{"key":"2025092505024324000_66.4.351.3","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2005.01.015"},{"key":"2025092505024324000_66.4.351.4","doi-asserted-by":"publisher","DOI":"10.1586\/erc.10.135"},{"key":"2025092505024324000_66.4.351.5","unstructured":"Lack EE . Atlas of tumor pathology, tumors of the adrenal gland and extra-adrenal paraganglia. Washington: The American Registry of Pathology, The Armed Forces Institute of Pathology, Ser. 4 Fasc. 8, 2007:112\u201314."},{"key":"2025092505024324000_66.4.351.6","unstructured":"DeLellis RA Lloyd RV Heitz PU Eng C , eds. World Health Organization classification of tumours. Pathology and genetics of tumours of endocrine organs. Lyon: IARC Press, 2004:143\u20136."},{"key":"2025092505024324000_66.4.351.7","doi-asserted-by":"publisher","DOI":"10.1530\/eje.0.1510073"},{"key":"2025092505024324000_66.4.351.8","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2009-2010"},{"key":"2025092505024324000_66.4.351.9","doi-asserted-by":"crossref","first-page":"10731","DOI":"10.1016\/S0021-9258(18)99077-0","article-title":"Aldosterone synthase cytochrome P-450 expressed in the adrenals of patients with primary aldosteronism","volume":"266","author":"Ogishima","year":"1991","journal-title":"J Biol Chem"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jclinpath-2012-201287","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T12:02:52Z","timestamp":1758801772000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jclinpath-2012-201287"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,2,22]]},"references-count":9,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2013,3,14]]},"published-print":{"date-parts":[[2013,4]]}},"alternative-id":["10.1136\/jclinpath-2012-201287"],"URL":"https:\/\/doi.org\/10.1136\/jclinpath-2012-201287","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"value":"0021-9746","type":"print"},{"value":"1472-4146","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,2,22]]}}}