{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T13:23:52Z","timestamp":1765545832577,"version":"3.44.0"},"reference-count":23,"publisher":"BMJ","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Clin Pathol"],"accepted":{"date-parts":[[2013,4,29]]},"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:sec>\n                  <jats:title>Aims<\/jats:title>\n                  <jats:p>Clonal expansion of lymphocytes may account for asymptomatic lymphocytosis. Unlike monoclonal B lymphocytosis, the detection of T-cell clones is difficult using immunophenotype-based assays. This study aimed to evaluate PCR-based clonality assays for the identification of T-cell clones among patients with unexplained lymphocytosis.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>We incorporated the BIOMED-2 multiplex PCR into the investigation of peripheral lymphocytosis. Clonality analysis was performed with three reactions for <jats:italic>TCR\u03b2<\/jats:italic> and two reactions for <jats:italic>TCR\u03b3<\/jats:italic> gene rearrangements. The analysis was performed with blood specimens from a total of 150 adult patients who presented with incidental lymphocytosis.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>PCR assays were validated using confirmed T-cell malignancies and the detection sensitivity was determined at a level of 2%. Using the <jats:italic>TCR\u03b2<\/jats:italic> and <jats:italic>TCR\u03b3<\/jats:italic> combination, 25 (16.6%) of 150 patients were found to have clonal <jats:italic>TCR<\/jats:italic> arrangement. Patients who harboured clonal T cells presented with a mild to moderate absolute lymphocytosis, with a median lymphocyte count of 4.5\u2005\u00d7\u200510<jats:sup>9<\/jats:sup>\/l (range 3.7\u20139.8\u2005\u00d7\u200510<jats:sup>9<\/jats:sup>) and the absence of other haematological abnormalities. Immunophenotyping confirmed T-cell lymphocytosis with an increase in CD8 T cells in the majority of patients.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>This study demonstrated the use of PCR assays for the effective detection of clonal T lymphocytosis. Our data indicate a high prevalence of silent T-cell clones among patients with peripheral lymphocytosis.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1136\/jclinpath-2012-201417","type":"journal-article","created":{"date-parts":[[2013,5,24]],"date-time":"2013-05-24T00:56:35Z","timestamp":1369356995000},"page":"749-752","source":"Crossref","is-referenced-by-count":4,"title":["T-cell clonality is detected in a high frequency among patients with incidental lymphocytosis by PCR assays for <i>TCR<\/i> gene rearrangements"],"prefix":"10.1136","volume":"66","author":[{"given":"Fenglin","family":"Cao","sequence":"first","affiliation":[{"name":"Department of Hematology, The First Hospital of Harbin Medical University, Harbin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuye","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Hematology, The First Hospital of Harbin Medical University, Harbin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Hematology, The First Hospital of Harbin Medical University, Harbin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Hematology, The First Hospital of Harbin Medical University, Harbin, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guisheng","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, University of Toronto, Toronto, Ontario, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Hematology, The First Hospital of Harbin Medical University, Harbin, China"},{"name":"Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, University of Toronto, Toronto, Ontario, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[2013,5,23]]},"reference":[{"key":"2025092503471530000_66.9.749.1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2005.05550.x"},{"key":"2025092503471530000_66.9.749.2","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPV2D1DDSGJDBW"},{"key":"2025092503471530000_66.9.749.3","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2009.175"},{"key":"2025092503471530000_66.9.749.4","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V98.1.165"},{"key":"2025092503471530000_66.9.749.5","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403202"},{"key":"2025092503471530000_66.9.749.6","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404467"},{"key":"2025092503471530000_66.9.749.7","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404481"},{"key":"2025092503471530000_66.9.749.8","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403428"},{"key":"2025092503471530000_66.9.749.9","first-page":"659","article-title":"Molecular immunoglobulin\/T-cell receptor clonality analysis in cutaneous lymphoproliferations. 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