{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T06:09:01Z","timestamp":1769148541255,"version":"3.49.0"},"reference-count":22,"publisher":"BMJ","issue":"8","content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["J Clin Pathol"],"accepted":{"date-parts":[[2015,4,21]]},"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:sec>\n                  <jats:title>Objective<\/jats:title>\n                  <jats:p>Problems in pre-cancer diagnosis complicate cancer theragnosis as well as life expectancy. There is uncertainty regarding malignant transformation of oral submucous fibrosis (OSF), an oral pre-cancer with dysplastic (OSFWD) and non-dysplastic (OSFWT) subtypes. Understanding the structural, molecular and physical aspects of epithelial homeostasis may be useful.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Materials and methods<\/jats:title>\n                  <jats:p>Histopathological grading of biopsy sections was performed using H&amp;E staining. Alterations in epithelial surface architecture in different groups was evaluated using scanning electron microscopy (SEM). The expression of crucial epithelial genes (p63, CK-5\/6, CK-10, E-cadherin and \u03b2-catenin) was studied by immunohistochemistry, Western blot and RT-PCR analysis.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>SEM observations revealed that the surface epithelial ridge pattern became thick and dense, and pit pattern gradually decreased in OSFWD and oral squamous cell carcinoma (OSCC). p63, \u0394Np63 and CK-5\/6 were up-regulated in OSFWD and OSCC but down-regulated in OSFWT. CK-10 was down-regulated in OSFWD compared to OSFWT. Cytoplasmic expression of E-cadherin and \u03b2-catenin was elevated in dysplastic and cancerous conditions. Moreover, statistical correlation between SEM features (ridges and pits) and molecular attributes demonstrated a significant positive relationship between the ridge-to-pit ratio and p63 population density (r=0.85) and the ridge-to-pit ratio and CK-5\/6 intensity (r=0.63).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Molecular changes related to epithelial progressive maturation and cellular proliferation are correlated with concomitant alteration of epithelial surface architecture which helps to predict the malignant potentiality of OSF.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1136\/jclinpath-2014-202791","type":"journal-article","created":{"date-parts":[[2015,6,2]],"date-time":"2015-06-02T22:35:39Z","timestamp":1433284539000},"page":"605-613","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":6,"title":["Connecting cyto-nano-architectural attributes and epithelial molecular expression in oral submucous fibrosis progression to cancer"],"prefix":"10.1136","volume":"68","author":[{"given":"Swarnendu","family":"Bag","sequence":"first","affiliation":[{"name":"School of Medical Science and Technology, Indian Institute of Technology, Kharagpur, West Bengal, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mousumi","family":"Pal","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Pathology, Guru Nanak Institute of Dental Sciences and Research, Kolkata, West Bengal, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amrita","family":"Chaudhary","sequence":"additional","affiliation":[{"name":"School of Medical Science and Technology, Indian Institute of Technology, Kharagpur, West Bengal, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raunak Kumar","family":"Das","sequence":"additional","affiliation":[{"name":"School of Bio Sciences and Technology & Centre for Biomaterials Science and Technology, Vellore Institute of Technology, VIT University, Vellore, Tamil Nadu, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ranjan Rashmi","family":"Paul","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Pathology, Guru Nanak Institute of Dental Sciences and Research, Kolkata, West Bengal, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanghamitra","family":"Sengupta","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Calcutta, Kolkata, West Bengal, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jyotirmoy","family":"Chatterjee","sequence":"additional","affiliation":[{"name":"School of Medical Science and Technology, Indian Institute of Technology, Kharagpur, West Bengal, 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