{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T03:03:35Z","timestamp":1789009415411,"version":"build-2803163510"},"reference-count":18,"publisher":"BMJ","issue":"7","content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["J Clin Pathol"],"accepted":{"date-parts":[[2015,3,29]]},"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Thalassaemia is a haemoglobin disorder caused by a reduction in, or a complete absence of, the production of \u03b1- or \u03b2-globin genes. Detection of \u03b2-thalassaemia carriers is the first step in the prenatal diagnosis of the disease and is based primarily on the differences between levels of blood cell indices. Since co-inheritance of \u03b2- and \u03b1-thalassaemia mutations modulates the haematological parameters of heterozygote \u03b2-thalassaemia indices, understanding the influence of this interaction is helpful for identification of disease carriers.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To determine the effects of \u03b1-thalassaemia mutations on the haematological parameters of \u03b2-thalassaemia carriers.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>We used gap-PCR and amplification refractory mutation system techniques to find any \u03b1- and\/or \u03b2-thalassaemia mutations in 270 subjects who were suspected to be thalassaemia carriers. The mean values of the haematological parameters in \u03b1, \u03b2-thalassaemia and \u03b2-thalassaemia carriers were compared.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Significant differences in mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and HbA2 were found between the two groups. Patients who were \u03b1, \u03b2-thalassaemia carriers had higher mean values of MCV and MCH, whereas HbA2 levels were higher in simple \u03b2-thalassaemia. No marked differences were found in mean cell haemoglobin (Hb) concentration and Hb blood cell indices. The value of MCV, MCH and HbA2 were significantly different between \u03b1,\u03b2-thalassaemia and simple \u03b2-thalassaemia in men and women, but the mean values of Hb in the two groups differed markedly only in men.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>We conclude that co-inheritance of \u03b1- and \u03b2-thalassaemia mutations may result in misdiagnosis of \u03b2-thalassaemia carriers. Therefore, in genetic counselling of patients with a near-normal range of blood cell indices the possibility that they may carry \u03b1, \u03b2-thalassaemia mutations must be considered.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/jclinpath-2014-202825","type":"journal-article","created":{"date-parts":[[2015,5,2]],"date-time":"2015-05-02T21:12:07Z","timestamp":1430601127000},"page":"562-566","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":23,"title":["Effects of \u03b1-thalassaemia mutations on the haematological parameters of \u03b2-thalassaemia carriers"],"prefix":"10.1136","volume":"68","author":[{"given":"Nasrollah","family":"Saleh-Gohari","sequence":"first","affiliation":[{"name":"Physiology Research Center, Kerman University of Medical Sciences, Kerman, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryam","family":"Khademi Bami","sequence":"additional","affiliation":[{"name":"Genetic Laboratory, Afzalipour Hospital, Kerman, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roya","family":"Nikbakht","sequence":"additional","affiliation":[{"name":"Department of Biostatistics and Epidemiology, Research Centre for Modelling in Health, Institute for Futures Studies in Health, Kerman University of Medical Sciences, Kerman, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hassan","family":"Karimi-Maleh","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Graduate University of Advanced Technology, Kerman, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2015,5,2]]},"reference":[{"key":"2025120618520120000_68.7.562.1","first-page":"649","article-title":"Genetic modifiers of \u03b2-thalassemia","volume":"90","author":"Thein","year":"2005","journal-title":"Haematologica"},{"key":"2025120618520120000_68.7.562.2","first-page":"69","article-title":"Distribution of \u03b2-globin gene mutations in thalassemia minor population of Kerman Province, Iran","volume":"39","author":"Saleh-Gohari","year":"2010","journal-title":"Iran J Public Health"},{"key":"2025120618520120000_68.7.562.3","unstructured":"Forget BG . Molecular genetics of the human globin genes. In: Steinberg MH , Forget BG , Higgs DR , Nager RL, eds . Disorders of haemoglobin. Genetic, pathophysiology and clinical management. Cambridge University Press, 2001:117\u201330."},{"key":"2025120618520120000_68.7.562.4","doi-asserted-by":"publisher","DOI":"10.3109\/03630269.2010.511587"},{"key":"2025120618520120000_68.7.562.5","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1002\/humu.9479","article-title":"HbVar database of human haemoglobin variants and thalassemia mutations: 2007 update","volume":"28","author":"Giardine","year":"2007","journal-title":"Hum Mutat"},{"key":"2025120618520120000_68.7.562.6","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.e228"},{"key":"2025120618520120000_68.7.562.7","first-page":"81","article-title":"Co-inheritance of sickle cell trait and thalassemia mutations in South central Iran","volume":"41","author":"Saleh-Gohari","year":"2012","journal-title":"Iran J Public Health"},{"key":"2025120618520120000_68.7.562.8","first-page":"856","article-title":"Analysis of hematological characteristics on the 79 co-inheritance of \u03b1-thalassemia and \u03b2-thalassemia carriers in Guangxi]","volume":"33","author":"Xiong","year":"2012","journal-title":"Zhonghua Xue Ye Xue Za Zhi"},{"key":"2025120618520120000_68.7.562.9","doi-asserted-by":"publisher","DOI":"10.1266\/ggs.84.67"},{"key":"2025120618520120000_68.7.562.10","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1982.tb03916.x"},{"key":"2025120618520120000_68.7.562.11","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/16.3.1215"},{"key":"2025120618520120000_68.7.562.12","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-8652(199808)58:4<306::AID-AJH10>3.0.CO;2-5"},{"key":"2025120618520120000_68.7.562.13","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/17.7.2503"},{"key":"2025120618520120000_68.7.562.14","doi-asserted-by":"publisher","DOI":"10.3109\/03630267909069153"},{"key":"2025120618520120000_68.7.562.15","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2008.056945"},{"key":"2025120618520120000_68.7.562.16","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.22188"},{"key":"2025120618520120000_68.7.562.17","doi-asserted-by":"crossref","first-page":"858294","DOI":"10.1155\/2013\/858294","article-title":"Hemoglobin A2 lowered by iron deficiency and \u03b1-thalassemia: should screening recommendation for \u03b2-thalassemia change?","volume":"2013","author":"Denic","year":"2013","journal-title":"ISRN Hematol"},{"key":"2025120618520120000_68.7.562.18","first-page":"81","article-title":"Co-inheritance of \u03b1-and \u03b2-thalassemia: challenges in prenatal diagnosis of thalassemia","volume":"2","author":"Kiani Moghaddam","year":"2012","journal-title":"IJBC"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jclinpath-2014-202825","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,7]],"date-time":"2025-12-07T02:52:13Z","timestamp":1765075933000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jclinpath-2014-202825"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,5,2]]},"references-count":18,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2015,6,19]]},"published-print":{"date-parts":[[2015,7]]}},"alternative-id":["10.1136\/jclinpath-2014-202825"],"URL":"https:\/\/doi.org\/10.1136\/jclinpath-2014-202825","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"value":"0021-9746","type":"print"},{"value":"1472-4146","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,5,2]]}}}