{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T15:59:26Z","timestamp":1774972766079,"version":"3.50.1"},"reference-count":24,"publisher":"BMJ","issue":"3","content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["J Clin Pathol"],"accepted":{"date-parts":[[2015,8,4]]},"published-print":{"date-parts":[[2016,3]]},"abstract":"<jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>\n                      <jats:italic>MYC<\/jats:italic>\n                      rearrangements with or without\n                      <jats:italic>BCL2<\/jats:italic>\n                      rearrangements have been shown to be associated with poor prognosis and inferior survival in diffuse large B-cell lymphomas (DLBCL). Most of these cases are still diagnosed by fluorescent in situ hybridisation (FISH) testing, which is expensive, requires expertise and is not routinely available in all laboratories. Immunohistochemistry (IHC) is widely available and has the potential to be used as a screening test to identify cases with increased protein expression and select cases that require confirmatory testing. We correlated the expression of\n                      <jats:italic>MYC<\/jats:italic>\n                      and\n                      <jats:italic>BCL2<\/jats:italic>\n                      by IHC with FISH studies in an attempt to define a cut-off value, which can be used by laboratories to select cases requiring confirmatory FISH testing. The prevalence of\n                      <jats:italic>MYC<\/jats:italic>\n                      -positive DLBCL and double-hit lymphoma (DHL) has also been studied.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      209 cases comprising of 15 cases of Burkitt lymphoma (BL), 13 cases of intermediate BL\/DLBCL and 181 cases of DLBCL were included. IHC and FISH for\n                      <jats:italic>MYC<\/jats:italic>\n                      and\n                      <jats:italic>BCL2<\/jats:italic>\n                      were performed and the results were correlated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The prevalence of\n                      <jats:italic>MYC<\/jats:italic>\n                      -positive DLBCL and\n                      <jats:italic>MYC<\/jats:italic>\n                      \/\n                      <jats:italic>BCL2<\/jats:italic>\n                      DHL was 13.4% and 7.4%, respectively, in our study. Germinal-centre subtype was more common in\n                      <jats:italic>MYC<\/jats:italic>\n                      -positive DLBCL and DHL.\n                      <jats:italic>MYC<\/jats:italic>\n                      -positive DLBCL also showed higher median Ki-67 (&gt;90%) and CD10 positivity as compared with\n                      <jats:italic>MYC<\/jats:italic>\n                      -negative cases.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      IHC can be used for screening cases, which require further confirmatory FISH testing. We recommend a cut-off value of \u226530% for\n                      <jats:italic>MYC<\/jats:italic>\n                      by IHC; however, international standardisation of these values is necessary to provide uniformity among laboratories.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/jclinpath-2015-203002","type":"journal-article","created":{"date-parts":[[2015,8,25]],"date-time":"2015-08-25T22:04:33Z","timestamp":1440540273000},"page":"266-270","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":30,"title":["Role of immunohistochemistry in the era of genetic testing in\n                    <i>MYC<\/i>\n                    -positive aggressive B-cell lymphomas: a study of 209 cases"],"prefix":"10.1136","volume":"69","author":[{"given":"Rishu","family":"Agarwal","sequence":"first","affiliation":[{"name":"Division of Cancer Medicine, Department of Pathology, Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen","family":"Lade","sequence":"additional","affiliation":[{"name":"Division of Cancer Medicine, Department of Pathology, Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danny","family":"Liew","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Haematology, Royal Melbourne Hospital, Parkville, Victoria, Australia"},{"name":"University of Melbourne, Department of Pathology, Parkville, Victoria, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toni-Maree","family":"Rogers","sequence":"additional","affiliation":[{"name":"Division of Cancer Medicine, Department of Pathology, Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Byrne","sequence":"additional","affiliation":[{"name":"Division of Cancer Medicine, Department of Pathology, Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank","family":"Feleppa","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Haematology, Royal Melbourne Hospital, Parkville, Victoria, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Surender","family":"Juneja","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Haematology, Royal Melbourne Hospital, Parkville, Victoria, Australia"},{"name":"University of Melbourne, Department of Pathology, Parkville, Victoria, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David A","family":"Westerman","sequence":"additional","affiliation":[{"name":"Division of Cancer Medicine, Department of Pathology, Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia"},{"name":"University of Melbourne, Department of Pathology, Parkville, Victoria, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[2015,8,25]]},"reference":[{"key":"2025120519160492000_69.3.266.1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199309303291402"},{"key":"2025120519160492000_69.3.266.2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2009.26.3947"},{"key":"2025120519160492000_69.3.266.3","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-05-220095"},{"key":"2025120519160492000_69.3.266.4","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-03-212191"},{"key":"2025120519160492000_69.3.266.5","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.11305"},{"key":"2025120519160492000_69.3.266.6","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2011.41.0985"},{"key":"2025120519160492000_69.3.266.7","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2011.41.4342"},{"key":"2025120519160492000_69.3.266.8","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-10-460063"},{"key":"2025120519160492000_69.3.266.9","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-06-435842"},{"key":"2025120519160492000_69.3.266.10","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.12763"},{"key":"2025120519160492000_69.3.266.11","doi-asserted-by":"publisher","DOI":"10.3109\/10428194.2013.806803"},{"key":"2025120519160492000_69.3.266.12","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2009.012864"},{"key":"2025120519160492000_69.3.266.13","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2011.08675.x"},{"key":"2025120519160492000_69.3.266.14","unstructured":"Swerdlow SH , Campo E , Haris NL , et al ., eds. 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