{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T02:11:07Z","timestamp":1790475067859,"version":"4.1.0"},"reference-count":31,"publisher":"BMJ","issue":"8","content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["J Clin Pathol"],"accepted":{"date-parts":[[2015,12,1]]},"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:sec>\n                  <jats:title>Aims<\/jats:title>\n                  <jats:p>Pulmonary toxoplasmosis has become a very rare parasitic infection since the advent of highly active antiretroviral therapies. It is generally diagnosed by the direct microscopic observation of <jats:italic>Toxoplasma gondii<\/jats:italic> tachyzoites in bronchoalveolar lavage fluid (BALF). The aim of this study was to assess possible improvements in diagnostic performance associated with the use of real-time PCR.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>This prospective study was carried out on BALFs obtained from immunocompromised patients over a 2-year period. We systematically compared the results of conventional staining with those of molecular detection.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Two cases of pulmonary toxoplasmosis were diagnosed for a total of 336 samples. PCR did not detect any additional cases and was more time-consuming than conventional staining.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Conventional staining is a reliable technique and is probably the most appropriate method for experienced microbiology laboratories, whereas <jats:italic>T. gondii<\/jats:italic>-specific PCR may be useful for laboratories with less experience in parasitology.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Trial registration number<\/jats:title>\n                  <jats:p>2015_030, May 27th 2015.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1136\/jclinpath-2015-203385","type":"journal-article","created":{"date-parts":[[2016,1,4]],"date-time":"2016-01-04T21:10:20Z","timestamp":1451941820000},"page":"726-730","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":15,"title":["Pulmonary toxoplasmosis in immunocompromised patients with interstitial pneumonia: a single-centre prospective study assessing PCR-based diagnosis"],"prefix":"10.1136","volume":"69","author":[{"given":"Guillaume","family":"Desoubeaux","sequence":"first","affiliation":[{"name":"Service de Parasitologie\u2014Mycologie\u2014M\u00e9decine tropicale, CHU de Tours, Tours, France"},{"name":"Facult\u00e9 de M\u00e9decine, Universit\u00e9 Fran\u00e7ois-Rabelais, CEPR\u2014INSERM U1100\/\u00c9quipe 3, Universit\u00e9 Fran\u00e7ois-Rabelais Tours, 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