{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T10:54:54Z","timestamp":1783508094905,"version":"3.55.0"},"reference-count":16,"publisher":"BMJ","issue":"9","content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["J Clin Pathol"],"accepted":{"date-parts":[[2016,1,13]]},"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>\n                      RT-qPCR is used to quantify minimal residual disease (MRD) in chronic myeloid leukaemia (CML) in order to make decisions on treatment, but its results depend on the level of\n                      <jats:italic>BCR-ABL1<\/jats:italic>\n                      expression as well as leukaemic cell number. The aims of the study were to quantify inter-individual differences in expression level, to determine the relationship between expression level and response to treatment, and to investigate the effect of expression level on interpretation of the RT-qPCR result.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      <jats:italic>BCR-ABL1<\/jats:italic>\n                      expression was studied in 248 samples from 65 patients with CML by determining the difference between MRD quantified by RT-qPCR and DNA-qPCR. The results were analysed statistically and by simple indicative modelling.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Inter-individual levels of expression approximated a normal distribution with an SD of 0.36 log. Expression at diagnosis correlated with expression during treatment. Response to treatment, as measured by the number of leukaemic cells after 3, 6 or 12\u2005months of treatment, was not related to the level of expression. Indicative modelling suggested that interpretation of RT-qPCR results in relation to treatment guidelines could be affected by variation in expression when MRD was around 10% at 3\u2005months and by both expression variation and Poisson variation when MRD was around or below the limit of detection of RT-qPCR.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Variation between individuals in expression of\n                      <jats:italic>BCR-ABL1<\/jats:italic>\n                      can materially affect interpretation of the RT-qPCR when this test is used to make decisions on treatment.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/jclinpath-2015-203538","type":"journal-article","created":{"date-parts":[[2016,2,2]],"date-time":"2016-02-02T22:49:40Z","timestamp":1454453380000},"page":"817-821","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":8,"title":["<i>BCR-ABL1<\/i>\n                    expression, RT-qPCR and treatment decisions in chronic myeloid leukaemia"],"prefix":"10.1136","volume":"69","author":[{"given":"Susan","family":"Latham","sequence":"first","affiliation":[{"name":"Department of Haematology & Genetic Pathology, School of Medicine, Flinders University and Medical Centre, Bedford Park, South Australia, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul A","family":"Bartley","sequence":"additional","affiliation":[{"name":"Department of Haematology & Genetic Pathology, School of Medicine, Flinders University and Medical Centre, Bedford Park, South Australia, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bradley","family":"Budgen","sequence":"additional","affiliation":[{"name":"Department of Haematology & Genetic Pathology, School of Medicine, Flinders University and Medical Centre, Bedford Park, South Australia, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David M","family":"Ross","sequence":"additional","affiliation":[{"name":"Department of Haematology & Genetic Pathology, School of Medicine, Flinders University and Medical Centre, Bedford Park, South Australia, Australia"},{"name":"Haematology Division, SA Pathology, Adelaide, South Australia, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Hughes","sequence":"additional","affiliation":[{"name":"Department of Haematology & Genetic Pathology, School of Medicine, Flinders University and Medical Centre, Bedford Park, South Australia, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan","family":"Branford","sequence":"additional","affiliation":[{"name":"Centre for Cancer Biology, SA Pathology, Adelaide, South Australia, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah","family":"White","sequence":"additional","affiliation":[{"name":"South Australian Health and Medical Research Institute, Adelaide, South Australia, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy P","family":"Hughes","sequence":"additional","affiliation":[{"name":"Haematology Division, SA Pathology, Adelaide, South Australia, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander A","family":"Morley","sequence":"additional","affiliation":[{"name":"Department of Haematology & Genetic Pathology, School of Medicine, Flinders University and Medical Centre, Bedford Park, South Australia, Australia"},{"name":"Monoquant Pty Ltd, Adelaide, South Australia, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2016,2,2]]},"reference":[{"key":"2025112109101883000_69.9.817.1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-04-150680"},{"key":"2025112109101883000_69.9.817.2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2014.10.002"},{"key":"2025112109101883000_69.9.817.3","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-03-1134"},{"key":"2025112109101883000_69.9.817.4","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-07-1112"},{"key":"2025112109101883000_69.9.817.5","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(67)90910-5"},{"key":"2025112109101883000_69.9.817.6","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2402912"},{"key":"2025112109101883000_69.9.817.7","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-05-501569"},{"key":"2025112109101883000_69.9.817.8","unstructured":"National Comprehensive Cancer Network. 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