{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T05:39:49Z","timestamp":1764999589251,"version":"3.46.0"},"reference-count":37,"publisher":"BMJ","issue":"12","content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["J Clin Pathol"],"accepted":{"date-parts":[[2016,4,24]]},"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>Differentiating between human papilloma virus-dependent vulvar low-grade and high-grade squamous intraepithelial lesions (LSILs and HSILs) remains difficult in selected cases. Stathmin, a protein involved in cell cycle progression, might be a useful additional marker for this differentiation. The aim of this study was to investigate the additional diagnostic value of stathmin expression in vulvar intraepithelial neoplastic (VIN) lesions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Immunohistochemical analysis was used to evaluate stathmin, P16 and Ki67 expression in 91 samples, including LSILs (n=16), HSILs (n=50), differentiated VIN (dVIN; n=10), lichen sclerosis (LS; n=10) and normal vulvar tissue (n=5).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Stathmin was expressed in more than one-third of the epithelium in all HSILs and in 20% of LSILs. P16 and Ki67 were expressed in more than one-third of the epithelium in 94% of HSILs and in 13% and 40% of LSILs, respectively. Stathmin was expressed in more than one-third of the epithelium in 10% of the dVIN and in none of the LS or normal lesions. P16 and Ki67 expression was not present in more than one-third of the epithelium in any of these lesions. The sensitivity of stathmin for differentiating between LSILs and HSILs was 100% compared with a sensitivity of 94% for both p16 and Ki67. The specificity of stathmin, p16 and Ki67 was 80%, 87% and 60%, respectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Stathmin is a highly sensitive and specific biomarker for the diagnosis of vulvar HSIL. In addition to the more commonly used immunohistochemical markers p16 and Ki67, stathmin can be a useful diagnostic tool for identifying HSILs, especially in cases in which differentiating between LSIL and HSIL is difficult.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/jclinpath-2016-203676","type":"journal-article","created":{"date-parts":[[2016,5,25]],"date-time":"2016-05-25T23:45:37Z","timestamp":1464219937000},"page":"1070-1075","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":10,"title":["Stathmin is a highly sensitive and specific biomarker for vulvar high-grade squamous intraepithelial lesions"],"prefix":"10.1136","volume":"69","author":[{"given":"Linda S","family":"Nooij","sequence":"first","affiliation":[{"name":"Department of Pathology, Leiden University Medical Center, Leiden, The Netherlands"},{"name":"Department of Gynaecology, Leiden University Medical Center, Leiden, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enno J","family":"Dreef","sequence":"additional","affiliation":[{"name":"Department of Pathology, Leiden University Medical Center, Leiden, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincent T H B M","family":"Smit","sequence":"additional","affiliation":[{"name":"Department of Pathology, Leiden University Medical Center, Leiden, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mari\u00ebtte I E","family":"van Poelgeest","sequence":"additional","affiliation":[{"name":"Department of Gynaecology, Leiden University Medical Center, Leiden, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tjalling","family":"Bosse","sequence":"additional","affiliation":[{"name":"Department of Pathology, Leiden University Medical Center, Leiden, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[2016,5,25]]},"reference":[{"key":"2025120521351889000_69.12.1070.1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-0528.2000.tb13298.x"},{"key":"2025120521351889000_69.12.1070.2","unstructured":"Kurman RJ , Carcangiu ML , Herrington CS , et al . WHO classification of tumours of female reproductive organs. Lyon: IARC, 2014."},{"key":"2025120521351889000_69.12.1070.3","first-page":"807","article-title":"Squamous vulvar intraepithelial neoplasia: 2004 modified terminology, ISSVD Vulvar Oncology Subcommittee","volume":"50","author":"Sideri","year":"2005","journal-title":"J Reprod Med"},{"key":"2025120521351889000_69.12.1070.4","doi-asserted-by":"publisher","DOI":"10.1097\/PGP.0b013e31826916c7"},{"key":"2025120521351889000_69.12.1070.5","unstructured":"Bornstein J . The 2015 International Society for the Study of Vulvovaginal Disease Terminology of Vulvar Squamous Intraepithelial Lesions. http:\/\/issvd.org\/wp-content\/uploads\/2015\/09\/2015-ISSVD-VIN-terminology-for-the-website-v5.pdf 2015. 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