{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T19:46:36Z","timestamp":1774467996126,"version":"3.50.1"},"reference-count":25,"publisher":"BMJ","issue":"1","content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["J Clin Pathol"],"accepted":{"date-parts":[[2016,5,24]]},"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:sec>\n                  <jats:title>Aims<\/jats:title>\n                  <jats:p>The presence of the \u03b6-globin chain is a good marker of (--<jats:sup>SEA<\/jats:sup>) \u03b1<jats:sup>0<\/jats:sup>-thalassaemia. We evaluated an immunochromatographic (IC) strip assay for \u03b6-globin in screening for (--<jats:sup>SEA<\/jats:sup>) \u03b1<jats:sup>0<\/jats:sup>-thalassaemia in a population with a high prevalence and heterogeneity of haemoglobinopathies.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>The study was carried out on 300 screen positive blood samples of Thai individuals. The IC strip assay for the \u03b6-globin chain was performed on all samples. The results were interpreted with thalassaemia genotyping using standard haemoglobin and DNA analyses.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Several thalassaemia genotypes were noted. Among the 300 subjects investigated, 79 had a positive IC strip assay for \u03b6-globin and (--<jats:sup>SEA<\/jats:sup>) \u03b1<jats:sup>0<\/jats:sup>-thalassaemia was identified in 40 of them. No (--<jats:sup>SEA<\/jats:sup>) \u03b1<jats:sup>0<\/jats:sup>-thalassaemia was detected in the remaining 39 samples with a positive IC strip test result or in the 221 samples with a negative IC strip test result. Further DNA analysis identified \u03b1<jats:sup>+<\/jats:sup>-thalassaemia in 25 of the 39 (--<jats:sup>SEA<\/jats:sup>) \u03b1<jats:sup>0<\/jats:sup>-thalassaemia negative samples. Using this IC strip assay in combination with a conventional screening protocol for (--<jats:sup>SEA<\/jats:sup>) \u03b1<jats:sup>0<\/jats:sup>-thalassaemia could provide sensitivity and specificity of 100% and 90.4%, respectively.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>IC strip assay for \u03b6-globin is simple, rapid and does not require sophisticated equipment. Use of this test in addition to the existing screening protocol could detect potential (--<jats:sup>SEA<\/jats:sup>) \u03b1<jats:sup>0<\/jats:sup>-thalassaemia leading to a significant reduction in the workload of DNA analysis. This could be used in areas where haemoglobinopathies are prevalent and heterogeneous but molecular testing is not available.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1136\/jclinpath-2016-203765","type":"journal-article","created":{"date-parts":[[2016,6,16]],"date-time":"2016-06-16T22:39:30Z","timestamp":1466116770000},"page":"63-68","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":11,"title":["Screening of (\u2013<sup>SEA<\/sup>) \u03b1-thalassaemia using an immunochromatographic strip assay for the \u03b6-globin chain in a population with a high prevalence and heterogeneity of haemoglobinopathies"],"prefix":"10.1136","volume":"70","author":[{"given":"Wittaya","family":"Jomoui","sequence":"first","affiliation":[{"name":"Biomedical Science Program, Graduate School, Khon Kaen, Thailand"},{"name":"Faculty of Associated Medical Sciences, Centre for Research and Development of Medical Diagnostic Laboratories, Khon Kaen University, Khon Kaen, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Goonnapa","family":"Fucharoen","sequence":"additional","affiliation":[{"name":"Faculty of Associated Medical Sciences, Centre for Research and Development of Medical Diagnostic Laboratories, Khon Kaen University, Khon Kaen, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kanokwan","family":"Sanchaisuriya","sequence":"additional","affiliation":[{"name":"Faculty of Associated Medical Sciences, Centre for Research and Development of Medical Diagnostic Laboratories, Khon Kaen University, Khon Kaen, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Supan","family":"Fucharoen","sequence":"additional","affiliation":[{"name":"Faculty of Associated Medical Sciences, Centre for Research and Development of Medical Diagnostic Laboratories, Khon Kaen University, Khon Kaen, 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