{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T03:20:13Z","timestamp":1790306413783,"version":"4.1.0"},"reference-count":19,"publisher":"BMJ","issue":"10","content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["J Clin Pathol"],"accepted":{"date-parts":[[2016,6,21]]},"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:p>Despite all the knowledge, the cellular and molecular mechanisms involved in myeloproliferative neoplasm (MPN) pathophysiology remain unclear. Authors have shown galectin-1 (Gal-1) and 3 playing roles in tumour angiogenesis and fibrosis, which were correlated with poor prognosis in patients with MPN. In the present study <jats:italic>LGALS1<\/jats:italic> and <jats:italic>LGALS3<\/jats:italic> were differently expressed between polycythemia vera, essential thrombocythemia (ET) and primary myelofibrosis (PMF) diseases. Increased <jats:italic>LGALS3<\/jats:italic> expression was associated with a negative JAK2 V617F status mutation in leucocytes from PMF but not in patients with ET without this mutation. However, a positive Janus kinase 2 (JAK2) V617F cell line established from patients with ET (SET-2 cells) when treated with JAK inhibitor presented high levels of <jats:italic>LGALS3.<\/jats:italic> Additionally, high <jats:italic>LGALS1<\/jats:italic> expression was found in CD34<jats:sup>+<\/jats:sup> cells but not in leucocytes from patients with PMF, in absence of JAK2 V617F mutation, and also in SET-2 cells treated with JAK inhibitor. Thus, our findings indicate that differential expression of <jats:italic>LGALS1<\/jats:italic> and\/or <jats:italic>LGALS3<\/jats:italic> in patients with MPN is linked with JAK2 V617F status mutation in these diseases and state of cell differentiation.<\/jats:p>","DOI":"10.1136\/jclinpath-2016-203948","type":"journal-article","created":{"date-parts":[[2016,7,12]],"date-time":"2016-07-12T06:50:56Z","timestamp":1468306256000},"page":"926-929","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":5,"title":["Different expression patterns of <i>LGALS1<\/i> and <i>LGALS3<\/i> in polycythemia vera, essential thrombocythemia and primary myelofibrosis"],"prefix":"10.1136","volume":"69","author":[{"given":"L G","family":"Moura","sequence":"first","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo, Ribeir\u00e3o Preto, S\u00e3o Paulo, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Tognon","sequence":"additional","affiliation":[{"name":"Departamento de Farm\u00e1cia, Universidade Federal de Juiz de Fora\u2014Campus Governador Valadares, Governador Valadares, Minas Gerais, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N S","family":"Nunes","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo, Ribeir\u00e3o Preto, S\u00e3o Paulo, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L Cataldi","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo, Ribeir\u00e3o Preto, S\u00e3o Paulo, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A F","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo, Ribeir\u00e3o Preto, S\u00e3o Paulo, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Kashima","sequence":"additional","affiliation":[{"name":"Hemocentro de Ribeir\u00e3o Preto-Hospital das Cl\u00ednicas, Faculdade de Medicina de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo, Ribeir\u00e3o Preto, S\u00e3o Paulo, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D T","family":"Covas","sequence":"additional","affiliation":[{"name":"Hemocentro de Ribeir\u00e3o Preto-Hospital das Cl\u00ednicas, Faculdade de Medicina de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo, Ribeir\u00e3o Preto, S\u00e3o Paulo, Brasil"},{"name":"Departamento de Cl\u00ednica M\u00e9dica, Faculdade de Medicina de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo, Ribeir\u00e3o Preto, S\u00e3o Paulo, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Santana","sequence":"additional","affiliation":[{"name":"Hospital Estadual de Transplantes Euryclides de Jesus Zerbini, S\u00e3o Paulo, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E X","family":"Souto","sequence":"additional","affiliation":[{"name":"Hospital Estadual de Transplantes Euryclides de Jesus Zerbini, S\u00e3o Paulo, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Perobelli","sequence":"additional","affiliation":[{"name":"Hospital Estadual de Transplantes Euryclides de Jesus Zerbini, S\u00e3o Paulo, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B P","family":"Sim\u00f5es","sequence":"additional","affiliation":[{"name":"Departamento de Cl\u00ednica M\u00e9dica, Faculdade de Medicina de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo, Ribeir\u00e3o Preto, S\u00e3o Paulo, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Dias-Baruffi","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo, Ribeir\u00e3o Preto, S\u00e3o Paulo, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F A","family":"Castro","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas, Toxicol\u00f3gicas e Bromatol\u00f3gicas, Faculdade de Ci\u00eancias Farmac\u00eauticas de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo, Ribeir\u00e3o Preto, S\u00e3o Paulo, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2016,7,11]]},"reference":[{"key":"2025092501410893000_69.10.926.1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-8722-5-2"},{"key":"2025092501410893000_69.10.926.2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-04-083501"},{"key":"2025092501410893000_69.10.926.3","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)71142-9"},{"key":"2025092501410893000_69.10.926.4","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-02-292102"},{"key":"2025092501410893000_69.10.926.5","first-page":"119","article-title":"The involvement of Galectins in the modulation of the JAK\/STAT pathway in myeloproliferative neoplasia","volume":"2","author":"Koopmans","year":"2012","journal-title":"Am J Blood Res."},{"key":"2025092501410893000_69.10.926.6","doi-asserted-by":"publisher","DOI":"10.3109\/07357907.2010.494319"},{"key":"2025092501410893000_69.10.926.7","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-540-46410-5_4"},{"key":"2025092501410893000_69.10.926.8","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1000154"},{"key":"2025092501410893000_69.10.926.9","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2401608"},{"key":"2025092501410893000_69.10.926.10","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2010.080895"},{"key":"2025092501410893000_69.10.926.11","first-page":"74","article-title":"Expression of galectin-1 in malignant tumors","volume":"31","author":"Demydenko","year":"2009","journal-title":"Exp Oncol"},{"key":"2025092501410893000_69.10.926.12","doi-asserted-by":"crossref","first-page":"983","DOI":"10.3892\/ijo.25.4.983","article-title":"Galectin-3 and cancer (Review)","volume":"25","author":"Califice","year":"2004","journal-title":"Int J Oncol"},{"key":"2025092501410893000_69.10.926.13","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-12-396457"},{"key":"2025092501410893000_69.10.926.14","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2012.315"},{"key":"2025092501410893000_69.10.926.15","first-page":"143","article-title":"Involvement of galectin-3 expression in colorectal cancer progression and metastasis","volume":"15","author":"Nakamura","year":"1999","journal-title":"Int J Oncol"},{"key":"2025092501410893000_69.10.926.16","doi-asserted-by":"publisher","DOI":"10.3109\/08923970902936880"},{"key":"2025092501410893000_69.10.926.17","first-page":"587","article-title":"Varied expression and localization of multiple galectins in different cancer cell lines","volume":"19","author":"Satelli","year":"2008","journal-title":"Oncol Rep"},{"key":"2025092501410893000_69.10.926.18","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2008.113"},{"key":"2025092501410893000_69.10.926.19","doi-asserted-by":"publisher","DOI":"10.1016\/j.jri.2011.04.004"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jclinpath-2016-203948","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T08:42:10Z","timestamp":1758789730000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jclinpath-2016-203948"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,7,11]]},"references-count":19,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2016,9,20]]},"published-print":{"date-parts":[[2016,10]]}},"alternative-id":["10.1136\/jclinpath-2016-203948"],"URL":"https:\/\/doi.org\/10.1136\/jclinpath-2016-203948","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"value":"0021-9746","type":"print"},{"value":"1472-4146","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,7,11]]}}}