{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T19:52:27Z","timestamp":1780602747628,"version":"3.54.1"},"reference-count":9,"publisher":"BMJ","issue":"3","funder":[{"name":"Children's Hospital of Michigan foundation"}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["J Clin Pathol"],"accepted":{"date-parts":[[2019,9,9]]},"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>To compare immunological microenvironments in local and distant lymphoid tissues in Hodgkin\u2019s lymphoma (HL) in children.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We have analysed diagnostic bone marrow (BM) samples in 22 and corresponding involved lymph node (LN) in eight and peripheral blood (PB) in eight cases of HL by flow cytometry and sought correlations with clinical features retrospectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>While there were significant differences in lymphocyte compositions of BM and LN tissues, the distribution of lymphocyte subsets mimicked each other in BM and PB. CD8-positive cytotoxic T cells predominate the bone marrow in contrast to CD4-positive helper T cells in LN tissue with corresponding CD4\/CD8 ratios (0.85 and 5.3, respectively; p=0.002). Additionally, T-large granular lymphocytes population was much higher in BM in comparison to LN tissue (10.5% vs 4.5%; p=0.036).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Local immunological microenvironment appears to be highly influenced by HL tumour cells and distant site lymphocyte composition reflects immune response to control the neoplastic process.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/jclinpath-2019-205967","type":"journal-article","created":{"date-parts":[[2019,9,21]],"date-time":"2019-09-21T17:15:13Z","timestamp":1569086113000},"page":"176-179","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":2,"title":["Differing reflections of paediatric classical Hodgkin\u2019s lymphoma on local and distant immunological microenvironments: a flow cytometric study"],"prefix":"10.1136","volume":"73","author":[{"given":"S\u00fcreyya","family":"Sava\u015fan","sequence":"first","affiliation":[{"name":"Hematology\/Oncology, Children\u2019s Hospital of Michigan, Wayne State University, Detroit, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0828-2471","authenticated-orcid":false,"given":"Batool","family":"AlQanber","sequence":"additional","affiliation":[{"name":"Hematology\/Oncology, Children\u2019s Hospital of Michigan, Wayne State University, Detroit, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meret","family":"Henry","sequence":"additional","affiliation":[{"name":"Hematology\/Oncology, Children\u2019s Hospital of Michigan, Wayne State University, Detroit, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven","family":"Buck","sequence":"additional","affiliation":[{"name":"Hematology\/Oncology, Children\u2019s Hospital of Michigan, Wayne State University, Detroit, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manisha","family":"Gadgeel","sequence":"additional","affiliation":[{"name":"Hematology\/Oncology, Children\u2019s Hospital of Michigan, Wayne State University, Detroit, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2019,9,21]]},"reference":[{"key":"2025121403320505000_73.3.176.1","doi-asserted-by":"publisher","DOI":"10.1002\/hon.878"},{"key":"2025121403320505000_73.3.176.2","doi-asserted-by":"crossref","DOI":"10.1002\/pbc.27307","article-title":"Flow cytometry for assessment of the tumor microenvironment in pediatric Hodgkin lymphoma","volume":"65","author":"Henry","year":"2018","journal-title":"Pediatr Blood Cancer"},{"key":"2025121403320505000_73.3.176.3","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2011.050138"},{"key":"2025121403320505000_73.3.176.4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2013\/658371","article-title":"Day 100 peripheral blood absolute Lymphocyte\/Monocyte ratio and survival in classical Hodgkin's lymphoma Postautologous peripheral blood hematopoietic stem cell transplantation","volume":"2013","author":"Porrata","year":"2013","journal-title":"Bone Marrow Res"},{"key":"2025121403320505000_73.3.176.5","doi-asserted-by":"crossref","first-page":"1276","DOI":"10.1016\/j.bbmt.2017.04.013","article-title":"Prognostic analysis of absolute lymphocyte and monocyte counts after autologous stem cell transplantation in children, adolescents, and young adults with refractory or relapsed Hodgkin lymphoma","volume":"23","author":"Galvez-Silva","year":"2017","journal-title":"Biol Blood Marrow Transplant"},{"key":"2025121403320505000_73.3.176.6","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2018-04-843714"},{"key":"2025121403320505000_73.3.176.7","doi-asserted-by":"publisher","DOI":"10.1182\/asheducation-2014.1.144"},{"key":"2025121403320505000_73.3.176.8","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0124531","article-title":"Macrophage polarization reflects T cell composition of tumor microenvironment in pediatric classical Hodgkin lymphoma and has impact on survival","volume":"10","author":"Barros","year":"2015","journal-title":"PLoS One"},{"key":"2025121403320505000_73.3.176.9","doi-asserted-by":"crossref","unstructured":"Sava\u015fan S , Wakeling E , Knight T , et al . Different clonal T-Large granular lymphocyte proliferations in SCID. J Clin Immunol 2019.","DOI":"10.1007\/s10875-019-00614-7"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jclinpath-2019-205967","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T11:32:16Z","timestamp":1765711936000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jclinpath-2019-205967"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9,21]]},"references-count":9,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2020,2,21]]},"published-print":{"date-parts":[[2020,3]]}},"alternative-id":["10.1136\/jclinpath-2019-205967"],"URL":"https:\/\/doi.org\/10.1136\/jclinpath-2019-205967","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"value":"0021-9746","type":"print"},{"value":"1472-4146","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,9,21]]}}}