{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T16:10:42Z","timestamp":1762272642563,"version":"3.44.0"},"reference-count":20,"publisher":"BMJ","issue":"11","license":[{"start":{"date-parts":[[2020,5,4]],"date-time":"2020-05-04T00:00:00Z","timestamp":1588550400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/bmj.com\/coronavirus\/usage"}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["J Clin Pathol"],"accepted":{"date-parts":[[2020,4,17]]},"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:p>In the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, pathologists can be exposed to infection handling surgical specimens. Guidelines related to safety procedures in the laboratory have been released. However, there is a lack of studies performed on biopsy and surgical resection specimens. Here we report the detection of SARS-CoV-2 in formalin-fixed paraffin-embedded samples from surgical resection of tongue squamous cell carcinoma of a patient who developed COVID-19 postsurgery. RNA of SARS-CoV-2 strain was detected in the tumour and the normal submandibular gland samples using real-time PCR-based assay. No viral RNA was found in metastatic and reactive lymph nodes. We demonstrated that SARS-CoV-2 RNA can be detected in routine histopathological samples even before COVID-19 disease development. These findings may give important information on the possible sites of infection or virus reservoir, and highlight the necessity of proper handling and fixation before sample processing.<\/jats:p>","DOI":"10.1136\/jclinpath-2020-206635","type":"journal-article","created":{"date-parts":[[2020,10,20]],"date-time":"2020-10-20T19:20:38Z","timestamp":1603221638000},"page":"754-757","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":30,"title":["SARS-CoV-2 detection in formalin-fixed paraffin-embedded tissue specimens from surgical resection of tongue squamous cell carcinoma"],"prefix":"10.1136","volume":"73","author":[{"given":"Elena","family":"Guerini-Rocco","sequence":"first","affiliation":[{"name":"Division of Pathology and Laboratory Medicine, IEO, European Institute of Oncology, IRCCS, Milano, Italy"},{"name":"Department of Oncology and Hemato-Oncology, University of Milano, Milano, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergio Vincenzo","family":"Taormina","sequence":"additional","affiliation":[{"name":"Division of Pathology and Laboratory Medicine, IEO, European Institute of Oncology, IRCCS, Milano, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Davide","family":"Vacirca","sequence":"additional","affiliation":[{"name":"Division of Pathology and Laboratory Medicine, IEO, European Institute of Oncology, IRCCS, Milano, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberto","family":"Ranghiero","sequence":"additional","affiliation":[{"name":"Division of Pathology and Laboratory Medicine, IEO, European Institute of Oncology, IRCCS, Milano, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandra","family":"Rappa","sequence":"additional","affiliation":[{"name":"Division of Pathology and Laboratory Medicine, IEO, European Institute of Oncology, IRCCS, Milano, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caterina","family":"Fumagalli","sequence":"additional","affiliation":[{"name":"Division of Pathology and Laboratory Medicine, IEO, European Institute of Oncology, IRCCS, Milano, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fausto","family":"Maffini","sequence":"additional","affiliation":[{"name":"Division of Pathology and Laboratory Medicine, IEO, European Institute of Oncology, IRCCS, Milano, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristiano","family":"Rampinelli","sequence":"additional","affiliation":[{"name":"Division of Radiology, IEO, European Institute of Oncology, IRCCS, Milano, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Domenico","family":"Galetta","sequence":"additional","affiliation":[{"name":"Division of Thoracic Surgery, IEO, European Institute of Oncology, IRCCS, Milano, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta","family":"Tagliabue","sequence":"additional","affiliation":[{"name":"Division of Otolaryngology and Head-Neck Surgery, IEO, European Institute of Oncology, IRCCS, Milano, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohssen","family":"Ansarin","sequence":"additional","affiliation":[{"name":"Division of Otolaryngology and Head-Neck Surgery, IEO, European Institute of Oncology, IRCCS, Milano, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0943-4804","authenticated-orcid":false,"given":"Massimo","family":"Barberis","sequence":"additional","affiliation":[{"name":"Division of Pathology and Laboratory Medicine, IEO, European Institute of Oncology, IRCCS, Milano, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[2020,5,4]]},"reference":[{"key":"2025092505561720000_73.11.754.1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30183-5"},{"key":"2025092505561720000_73.11.754.2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2001017"},{"key":"2025092505561720000_73.11.754.3","doi-asserted-by":"crossref","DOI":"10.1016\/S0140-6736(20)30644-9","article-title":"COVID-19: protecting health-care workers","volume":"395","year":"2020","journal-title":"Lancet"},{"key":"2025092505561720000_73.11.754.4","unstructured":"Prioritisation\/deferral of Pathology Laboratory Work (in light of SARSCoV-2 (COVID19) epidemic) . Recommendations from RCPath and professional bodies (IBMS, ACP and ACB). Available: https:\/\/www.rcpath.org\/uploads\/assets\/f5123842-950f-49c5-bf69ed866a7ca3da\/Prioritisation deferral-of-pathology-laboratory-work.pdf"},{"key":"2025092505561720000_73.11.754.5","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2020.1585"},{"key":"2025092505561720000_73.11.754.6","doi-asserted-by":"publisher","DOI":"10.1002\/hed.25466"},{"key":"2025092505561720000_73.11.754.7","unstructured":"Amin M , Edge S , Greene F . Ajcc cancer staging manual. 8. New York: Springer, 2017."},{"key":"2025092505561720000_73.11.754.8","first-page":"30132","article-title":"Pulmonary pathology of early-phase 2019 novel coronavirus (COVID-19) pneumonia in two patients with lung cancer","volume":"S1556-0864","author":"Tian","year":"2020","journal-title":"J Thorac Oncol"},{"key":"2025092505561720000_73.11.754.9","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2017.01.014"},{"key":"2025092505561720000_73.11.754.10","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2008.09.003"},{"key":"2025092505561720000_73.11.754.11","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2016-0397-SA"},{"key":"2025092505561720000_73.11.754.12","doi-asserted-by":"publisher","DOI":"10.1126\/science.275.5307.1793"},{"key":"2025092505561720000_73.11.754.13","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(18)30359-1"},{"key":"2025092505561720000_73.11.754.14","doi-asserted-by":"publisher","DOI":"10.1038\/s41368-020-0075-9"},{"key":"2025092505561720000_73.11.754.15","article-title":"Consistent detection of 2019 novel coronavirus in saliva","author":"To","year":"2020","journal-title":"Clin Infect Dis"},{"key":"2025092505561720000_73.11.754.16","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciy137"},{"key":"2025092505561720000_73.11.754.17","unstructured":"Gestione del rischio biologico correlato alla epidemia di COVID-19 nella manipolazione dei campioni tissutali E citologici. con particolare riguardo ai campioni a fresco o non adeguatamente fissati."},{"key":"2025092505561720000_73.11.754.18","unstructured":"Coronavirus Disease 2019 (COVID-19) . Interim laboratory biosafety guidelines for handling and processing specimens associated with coronavirus disease 2019 (COVID-19) print page, 2020. Available: https:\/\/www.cdc.gov\/coronavirus\/2019-ncov\/lab\/lab-biosafety-guidelines.html"},{"key":"2025092505561720000_73.11.754.19","doi-asserted-by":"crossref","unstructured":"Iwen PC , Stiles KL , Pentella MA . Safety considerations in the laboratory testing of specimens suspected or known to contain the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Am J Clin Pathol 2020.","DOI":"10.1093\/labmed\/lmaa018"},{"key":"2025092505561720000_73.11.754.20","unstructured":"College of American Pathologists . COVID19 - Remote Sign-Out Guidance. Available: https:\/\/documents.cap.org\/documents\/COVID19-Remote-Sign-Out-Guidance-vFNL.pdf"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jclinpath-2020-206635","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T12:56:25Z","timestamp":1758804985000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jclinpath-2020-206635"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,4]]},"references-count":20,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2020,10,20]]},"published-print":{"date-parts":[[2020,11]]}},"alternative-id":["10.1136\/jclinpath-2020-206635"],"URL":"https:\/\/doi.org\/10.1136\/jclinpath-2020-206635","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"type":"print","value":"0021-9746"},{"type":"electronic","value":"1472-4146"}],"subject":[],"published":{"date-parts":[[2020,5,4]]}}}