{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T03:44:09Z","timestamp":1772163849940,"version":"3.50.1"},"reference-count":18,"publisher":"BMJ","issue":"1","license":[{"start":{"date-parts":[[2020,11,3]],"date-time":"2020-11-03T00:00:00Z","timestamp":1604361600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["J Clin Pathol"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:p>With the global outbreak of COVID-19, the demand for testing rapidly increased and quickly exceeded the testing capacities of many laboratories. Clinical tests which receive CE (Conformit\u00e9 Europ\u00e9enne) and Food and Drug Administration (FDA) authorisations cannot always be tested thoroughly in a real-world environment. Here we demonstrate the long-term stability of nasopharyngeal swab specimens for SARS-CoV-2 molecular testing across three assays recently approved by the US FDA under Emergency Use Authorization. This study demonstrates that nasopharyngeal swab specimens can be stored under refrigeration or even ambient conditions for 21 days without clinically impacting the results of the real-time reverse transcriptase-PCR testing.<\/jats:p>","DOI":"10.1136\/jclinpath-2020-206738","type":"journal-article","created":{"date-parts":[[2020,11,3]],"date-time":"2020-11-03T17:20:48Z","timestamp":1604424048000},"page":"61-64","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":11,"title":["Extended storage of SARS-CoV-2 nasopharyngeal swabs does not negatively impact results of molecular-based testing across three clinical platforms"],"prefix":"10.1136","volume":"75","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2212-3533","authenticated-orcid":false,"given":"Karin A","family":"Skalina","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6367-8335","authenticated-orcid":false,"given":"D Y","family":"Goldstein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaffar","family":"Sulail","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eunkyu","family":"Hahm","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Momka","family":"Narlieva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wendy","family":"Szymczak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amy S","family":"Fox","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[2020,11,3]]},"reference":[{"key":"2021121513050727000_75.1.61.1","unstructured":"US Food & Drug Administration . Emergency use authorization, 2020. Available: https:\/\/www.fda.gov\/emergency-preparedness-and-response\/mcm-legal-regulatory-and-policy-framework\/emergency-use-authorization"},{"key":"2021121513050727000_75.1.61.2","unstructured":"Care DoHaS . Coronavirus (COVID-19): scaling up our testing programs, 2020."},{"key":"2021121513050727000_75.1.61.3","doi-asserted-by":"crossref","unstructured":"Richterich P . Severe underestimation of COVID-19 case numbers: effect of epidemic growth rate and test restrictions. medRxiv 2020.","DOI":"10.1101\/2020.04.13.20064220"},{"key":"2021121513050727000_75.1.61.4","doi-asserted-by":"crossref","first-page":"1070","DOI":"10.1515\/cclm-2020-0285","article-title":"Potential preanalytical and analytical vulnerabilities in the laboratory diagnosis of coronavirus disease 2019 (COVID-19)","volume":"58","author":"Lippi","year":"2020","journal-title":"Clin Chem Lab Med"},{"key":"2021121513050727000_75.1.61.5","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.06551-11"},{"key":"2021121513050727000_75.1.61.6","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.24549"},{"key":"2021121513050727000_75.1.61.7","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1016\/j.jmoldx.2020.04.209","article-title":"Detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is comparable in clinical samples preserved in saline or viral transport medium","volume":"22","author":"Radbel","year":"2020","journal-title":"J Mol Diagn"},{"key":"2021121513050727000_75.1.61.8","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.00708-20","article-title":"Evaluation of transport media and specimen transport conditions for the detection of SARS-CoV-2 by use of real-time reverse transcription-PCR","volume":"58","author":"Rogers","year":"2020","journal-title":"J Clin Microbiol"},{"key":"2021121513050727000_75.1.61.9","doi-asserted-by":"crossref","DOI":"10.1002\/jcla.23411","article-title":"Heat inactivation of serum interferes with the immunoanalysis of antibodies to SARS-CoV-2","volume":"34","author":"Hu","year":"2020","journal-title":"J Clin Lab Anal"},{"key":"2021121513050727000_75.1.61.10","unstructured":"Luminex Corporation . Aries SARS-CoV-2 assay (EUA-IVD), 2020."},{"key":"2021121513050727000_75.1.61.11","unstructured":"Abbott Laboratories . Abbott RealTime SARS-CoV-2: Instructions for use. Abbott molecular technical services 2020."},{"key":"2021121513050727000_75.1.61.12","unstructured":"Hologic . SARS-CoV-2 assay (Panther fusion system. contract No. AW-21159-001 Rev. 003), 2020."},{"key":"2021121513050727000_75.1.61.13","unstructured":"Roser M , Ritchie H , Ortiz-Ospina E , et al . Coronavirus pandemic (COVID-19) OurWorldInData.org, 2020. Available: https:\/\/ourworldindata.org\/coronavirus"},{"key":"2021121513050727000_75.1.61.14","doi-asserted-by":"crossref","DOI":"10.1128\/JCM.00783-20","article-title":"Clinical evaluation of three sample-to-answer platforms for detection of SARS-CoV-2","volume":"58","author":"Zhen","year":"2020","journal-title":"J Clin Microbiol"},{"key":"2021121513050727000_75.1.61.15","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc2004973"},{"key":"2021121513050727000_75.1.61.16","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1093\/infdis\/jiaa272","article-title":"Influence of storage conditions on SARS-CoV-2 nucleic acid detection in throat swabs","volume":"222","author":"Li","year":"2020","journal-title":"J Infect Dis"},{"key":"2021121513050727000_75.1.61.17","doi-asserted-by":"crossref","unstructured":"Yang Y , Yang M , Shen C , et al . Evaluating the accuracy of different respiratory specimens in the laboratory diagnosis and monitoring the viral shedding of 2019-nCoV infections. medRxiv 2020.","DOI":"10.1101\/2020.02.11.20021493"},{"key":"2021121513050727000_75.1.61.18"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jclinpath-2020-206738","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,11,26]],"date-time":"2022-11-26T07:38:46Z","timestamp":1669448326000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jclinpath-2020-206738"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11,3]]},"references-count":18,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2021,12,15]]},"published-print":{"date-parts":[[2022,1]]}},"alternative-id":["10.1136\/jclinpath-2020-206738"],"URL":"https:\/\/doi.org\/10.1136\/jclinpath-2020-206738","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.05.16.20104158","asserted-by":"object"}]},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"value":"0021-9746","type":"print"},{"value":"1472-4146","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,11,3]]}}}