{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T09:57:01Z","timestamp":1790935021533,"version":"4.1.0"},"reference-count":37,"publisher":"BMJ","issue":"9","content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["J Clin Pathol"],"accepted":{"date-parts":[[2022,3,25]]},"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>\n                      Risk stratification as per the International Neuroblastoma Risk Group (INRG) stratification is important for management of neuroblastoma. INRG incorporates various parameters including histological category as per the International Neuroblastoma Pathology Classification (INPC) and\n                      <jats:italic>MYCN<\/jats:italic>\n                      amplification, which were evaluated in fine needle aspiration biopsy (FNAB) samples of neuroblastoma patients to ascertain their impact in our population.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      This was a retrospective study including 60 neuroblastoma cases diagnosed on FNAB, staged and stratified by INRG. Mitosis Karyorrhexis Index (MKI), INPC morphological category and\n                      <jats:italic>MYCN<\/jats:italic>\n                      status by fluorescence in situ hybridisation (n=46) were evaluated and correlated to outcome.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The mean age was 29 months (21 days to 9 years) with 27 and 33 children &lt;\/\u226518 months; male: female ratio of 1.6: 1; INRG stage-30(M), 20(L2), 2(L1) and 2(MS); INRG-36 high-risk, 13 intermediate-risk and 11 low-risk categories, respectively. MKI was high, intermediate and low in 39, 4 and 7 cases, respectively. INPC morphological type included 2 ganglioneuroblastomas and 58 neuroblastomas, graded further as 25 undifferentiated and 33 poorly differentiated tumours.\n                      <jats:italic>MYCN<\/jats:italic>\n                      was amplified in 48% (22\/46) cases and correlated with undifferentiated morphology (p=0.01). At a mean follow-up of 469 (7-835) days, 22\/50 were disease free and 28\/50 had relapsed\/died. The overall survival correlated with age (p=0.03), stage (p=0.01), INRG group (p=0.0001) and tumour grade (p=0.036).\n                      <jats:italic>MYCN<\/jats:italic>\n                      status independently did not correlate with age (p=0.5), INRG stage (p=0.2) and overall survival (p=0.4).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      FNAB is a complete modality for diagnosing neuroblastoma and providing all information required for risk stratification as per INRG including MKI,\n                      <jats:italic>MYCN<\/jats:italic>\n                      amplification, INPC category. Our cohort with predominant high-risk neuroblastoma cases highlights regional variation.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/jclinpath-2022-208177","type":"journal-article","created":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T14:01:48Z","timestamp":1649772108000},"page":"599-605","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":6,"title":["<i>MYCN<\/i>\n                    amplification and International Neuroblastoma Risk Group stratification on fine-needle aspiration biopsy and their correlation to survival in neuroblastoma"],"prefix":"10.1136","volume":"76","author":[{"given":"Neha","family":"Bhardwaj","sequence":"first","affiliation":[{"name":"Pathology, Post Graduate Institute of Medical Education and Research, Chandigarh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manish","family":"Rohilla","sequence":"additional","affiliation":[{"name":"Cytology & Gynecological Pathology, Post Graduate Institute of Medical Education and Research, Chandigarh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amita","family":"Trehan","sequence":"additional","affiliation":[{"name":"Department of Pediatrics (Hematology-Oncology Division), Post Graduate Institute of Medical Education and Research, Chandigarh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deepak","family":"Bansal","sequence":"additional","affiliation":[{"name":"Department of Pediatrics (Hematology-Oncology Division), Post Graduate Institute of Medical Education and Research, Chandigarh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nandita","family":"Kakkar","sequence":"additional","affiliation":[{"name":"Histopathology, Post Graduate Institute of Medical Education and Research, Chandigarh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1771-091X","authenticated-orcid":false,"given":"Radhika","family":"Srinivasan","sequence":"additional","affiliation":[{"name":"Cytology & Gynecological Pathology, Post Graduate Institute of Medical Education and Research, Chandigarh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2022,4,12]]},"reference":[{"key":"2025121401001309000_76.9.599.1","unstructured":"Brodeur GHM , Bagatell R , et al . Neuroblastoma. In: Pizzo P , Poplack D , eds. Principles and practice of pediatric oncology. 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