{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T05:19:28Z","timestamp":1781587168267,"version":"3.54.5"},"reference-count":17,"publisher":"BMJ","issue":"6","funder":[{"DOI":"10.13039\/501100005247","name":"University of British Columbia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005247","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the Canadian Institutes of Health Research, the Micronutrient Initiative and Sight and Life Foundation"},{"DOI":"10.13039\/501100000245","name":"Michael Smith Foundation for Health Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000245","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["J Clin Pathol"],"accepted":{"date-parts":[[2022,8,27]]},"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:sec>\n                  <jats:title>Aim<\/jats:title>\n                  <jats:p>We investigated the potential of reticulocyte haemoglobin equivalent (RET-He) as an early marker of responsiveness to iron supplementation.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Data were obtained from a randomised controlled trial of daily iron supplementation in 356 Cambodian women (18\u201345 y) who received 60 mg elemental iron for 12 weeks. A fasted venous blood specimen was collected at baseline, 1-week and 12-week timepoints. Whole blood haemoglobin (g\/L) and RET-He (pg) were measured using a Sysmex haematology analyser. RET-He measures were evaluated for their predictive ability on haemoglobin response to iron supplementation (defined as \u226510 g\/L at 12 weeks). Receiver operating characteristic (ROC) curves were used to assess discrimination performance, and the area under the ROC curve (AUC<jats:sup>ROC<\/jats:sup>) served as a measure of the ability of each predictor to discriminate between women likely or unlikely to elicit a haemoglobin response.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Predictive ability (AUC<jats:sup>ROC<\/jats:sup> (95% CI)) of baseline, 1-week, and change from baseline to 1-week RET-He on haemoglobin response was 0.70 (0.63 to 0.76), 0.48 (0.41 to 0.56) and 0.81 (0.75 to 0.87), respectively. Based on the Youden index, an absolute increase in RET-He of ~1.1 pg or a percentage increase of ~4.4% over 1 week were optimal thresholds to predict responsiveness to iron supplementation.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>Single timepoint RET-He measures have poor predictive ability; however, change in RET-He after 1 week was a strong predictor of haemoglobin response among Cambodian women receiving 60 mg elemental iron and can be measured easily and quickly after only 1 week of iron therapy.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1136\/jcp-2021-208077","type":"journal-article","created":{"date-parts":[[2022,9,8]],"date-time":"2022-09-08T13:21:34Z","timestamp":1662643294000},"page":"407-412","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":4,"title":["Reticulocyte haemoglobin equivalent (RET-He) as an early marker of responsiveness to oral iron supplementation"],"prefix":"10.1136","volume":"76","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1168-7990","authenticated-orcid":false,"given":"Lulu X","family":"Pei","sequence":"first","affiliation":[{"name":"Food, Nutrition and Health, The University of British Columbia, Vancouver, British Columbia, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hou","family":"Kroeun","sequence":"additional","affiliation":[{"name":"Helen Keller International, Phnom Penh, Cambodia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1025-2194","authenticated-orcid":false,"given":"Crystal D","family":"Karakochuk","sequence":"additional","affiliation":[{"name":"Food, Nutrition and Health, The University of British Columbia, Vancouver, British Columbia, Canada"},{"name":"BC Children's Hospital Research Institute, Vancouver, British Columbia, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2022,9,8]]},"reference":[{"key":"2025092509340129000_76.6.407.1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.21090"},{"key":"2025092509340129000_76.6.407.2","doi-asserted-by":"publisher","DOI":"10.1080\/10408360091174196"},{"key":"2025092509340129000_76.6.407.3","doi-asserted-by":"crossref","first-page":"1369","DOI":"10.1515\/CCLM.2010.292","article-title":"Automated reticulocyte counting: state of the art and clinical applications in the evaluation of erythropoiesis","volume":"48","author":"Piva","year":"2010","journal-title":"Clin Chem Lab Med"},{"key":"2025092509340129000_76.6.407.4","unstructured":"World Health Organization . Assessing the iron status of populations. 2nd ed. Geneva: World Health Organization, 2007."},{"key":"2025092509340129000_76.6.407.5","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2257.2006.00812.x"},{"key":"2025092509340129000_76.6.407.6","doi-asserted-by":"crossref","first-page":"3100","DOI":"10.1182\/blood.V83.10.3100.3100","article-title":"Reticulocyte hemoglobin content (CHR): early indicator of iron deficiency and response to therapy","volume":"83","author":"Brugnara","year":"1994","journal-title":"Blood"},{"key":"2025092509340129000_76.6.407.7","doi-asserted-by":"crossref","first-page":"1510","DOI":"10.1016\/j.mayocp.2020.10.042","article-title":"Using reticulocyte hemoglobin equivalent as a marker for iron deficiency and responsiveness to iron therapy","volume":"96","author":"Auerbach","year":"2021","journal-title":"Mayo Clin Proc"},{"key":"2025092509340129000_76.6.407.8","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.117.155937"},{"key":"2025092509340129000_76.6.407.9","doi-asserted-by":"crossref","first-page":"2255","DOI":"10.1093\/jn\/nxab108","article-title":"Baseline hemoglobin, hepcidin, ferritin, and total body iron stores are equally strong diagnostic predictors of a hemoglobin response to 12 weeks of daily iron supplementation in Cambodian women","volume":"151","author":"Pei","year":"2021","journal-title":"J Nutr"},{"key":"2025092509340129000_76.6.407.10","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.116.140996"},{"key":"2025092509340129000_76.6.407.11","doi-asserted-by":"crossref","first-page":"3127","DOI":"10.1093\/jn\/134.11.3127","article-title":"Combined measurement of ferritin, soluble transferrin receptor, retinol binding protein, and C-reactive protein by an inexpensive, sensitive, and simple sandwich enzyme-linked immunosorbent assay technique","volume":"134","author":"Erhardt","year":"2004","journal-title":"J Nutr"},{"key":"2025092509340129000_76.6.407.12","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.116.141762"},{"key":"2025092509340129000_76.6.407.13","unstructured":"World Health Organization . Iron deficiency anaemia: assessment, prevention and control. Geneva: World Health Organization, 2001."},{"key":"2025092509340129000_76.6.407.14","doi-asserted-by":"publisher","DOI":"10.1097\/JTO.0b013e3181ec173d"},{"key":"2025092509340129000_76.6.407.15","doi-asserted-by":"publisher","DOI":"10.1111\/j.1651-2227.2006.00178.x"},{"key":"2025092509340129000_76.6.407.16","unstructured":"WHO . Guideline: daily iron supplementation in adult women and adolescent girls. Geneva: World Health Organization, 2016."},{"key":"2025092509340129000_76.6.407.17","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.23354"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp-2021-208077","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T16:34:09Z","timestamp":1758818049000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp-2021-208077"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,8]]},"references-count":17,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2023,5,18]]},"published-print":{"date-parts":[[2023,6]]}},"alternative-id":["10.1136\/jcp-2021-208077"],"URL":"https:\/\/doi.org\/10.1136\/jcp-2021-208077","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"value":"0021-9746","type":"print"},{"value":"1472-4146","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,8]]}}}