{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T17:45:38Z","timestamp":1789839938116,"version":"4.0.1"},"reference-count":36,"publisher":"BMJ","issue":"6","funder":[{"name":"Shanghai Sixth People's Hospital","award":["ynlc201727"],"award-info":[{"award-number":["ynlc201727"]}]},{"DOI":"10.13039\/501100001809","name":"National Nature Science Foundation of China","doi-asserted-by":"crossref","award":["81972500"],"award-info":[{"award-number":["81972500"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["20Z11900304"],"award-info":[{"award-number":["20Z11900304"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine"}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["J Clin Pathol"],"accepted":{"date-parts":[[2022,12,27]]},"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:sec>\n                  <jats:title>Aims<\/jats:title>\n                  <jats:p>To elucidate the clinicopathological features and the diagnostic value of mutation specific antibody H3F3 K36M of chondroblastoma (CB) in China.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Clinicopathological profiles were retrieved, and immunohistochemistry was performed on 185 CB specimens and the control group.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Our series included 307 patients with a mean age of 22.1 years. Long tubular bones (63.8%, 196\/307) were most commonly involved, followed by short bones of the hands and feet (22.1%, 68\/307), sesamoid bones (8.1%, 25\/307), flat bones and irregular bones (5.9%, 18\/307). The most commonly involved site was the proximal femur, followed by distal femur, proximal humerus and calcaneus. The average age in the long bones group (20.3 years) was significantly younger than the short bones group (24.9 years) (p&lt;0.001), sesamoid bones group (24.4 years) (p=0.02) and flat bones and irregular bones group (29.1 years) (p&lt;0.001). Microscopically, aneurysmal bone cyst-like change (63.6%, 117\/184), necrosis (43.5%, 80\/184) and chicken-wire calcification (26.1%, 48\/184) were variably noted. In rare cases, cortical destruction, soft tissue and lymphovascular invasion were identified. Positive immunoreaction with H3F3 K36M was examined in all non-decalcified, all EDTA decalcified, 87.1% hydrochloric acid (HCl) decalcified CB samples and the high-grade sarcoma secondary to CB, but not the control group.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>CB usually involves the long tubular bones in younger age group. H3F3 K36M can identify K36M mutation with 100% specificity and 100% sensitivity in non-decalcified and EDTA decalcified samples, more than 80% sensitivity in HCl decalcified samples. Virtually, all CBs harbour an H3K36M mutation.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1136\/jcp-2022-208630","type":"journal-article","created":{"date-parts":[[2023,1,5]],"date-time":"2023-01-05T13:22:25Z","timestamp":1672924945000},"page":"367-373","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":11,"title":["Chondroblastoma: clinicopathological analyses of 307 cases from a single institution in China and the diagnostic value of the H3F3 K36M mutant antibody"],"prefix":"10.1136","volume":"76","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6117-8465","authenticated-orcid":false,"given":"Qianqian","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Pathology, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Pathology, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanli","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Pathology, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Pathology, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dingjun","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Radiology, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4032-0116","authenticated-orcid":false,"given":"Jinyu","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Radiology, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Pathology, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huizhen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathology, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9668-7756","authenticated-orcid":false,"given":"Zhiyan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pathology, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2023,1,5]]},"reference":[{"key":"2025092501441053000_76.6.367.1","first-page":"969","article-title":"Benign chondroblastoma of bone: a reinterpretation of the so-called calcifying or chondromatous giant cell tumor","volume":"18","author":"Jaffe","year":"1942","journal-title":"Am J Pathol"},{"key":"2025092501441053000_76.6.367.2","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-197910000-00007"},{"key":"2025092501441053000_76.6.367.3","doi-asserted-by":"publisher","DOI":"10.1007\/s002560050567"},{"key":"2025092501441053000_76.6.367.4","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19850415)55:8<1770::AID-CNCR2820550825>3.0.CO;2-Q"},{"key":"2025092501441053000_76.6.367.5","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-020-0604-2"},{"key":"2025092501441053000_76.6.367.6","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1302\/0301-620X.52B4.741","article-title":"Benign chondroblastoma of bone. 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