{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T18:17:20Z","timestamp":1791656240804,"version":"4.3.4"},"reference-count":18,"publisher":"BMJ","issue":"9","content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["J Clin Pathol"],"accepted":{"date-parts":[[2024,2,11]]},"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>Accurate determination of histological activity in ulcerative colitis (UC) is essential given its diagnostic and prognostic importance. Data on the relationship between histology and immune cell markers are limited. We aimed to evaluate the association between histological disease activity and immune cell marker concentration in colonic biopsies from patients with UC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Sigmoid colon biopsies from 20 patients with UC were retrospectively assessed using the Robarts Histopathology Index (RHI). Targeted mass spectrometry determined the concentration of 18 immune cell markers (cluster of differentiation (CD) 4, CD8, CD19, CD20, CD40, CD56, CD68, CD103, forkhead box p3 (FOXP3), human leucocyte antigen, DR alpha chain (HLA-DRA), interleukin 10 (IL-10), IL-23 subunit alpha (IL-23A), IL-23 receptor (IL-23R), IL-2 receptor alpha chain (IL-2RA), Ki67, lymphocyte-activation gene 3 (LAG-3), programmed cell death protein 1 (PD-1) and PD ligand 1 (PD-L1)). The association between RHI score and immune cell marker concentration was quantified using Spearman\u2019s rank correlation coefficient (\u03c1) and related 95% CIs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Fourteen of the 18 immune cell marker proteins were detected, with tissue concentration ranging from 0.003 to 11.53\u2009fmol\/\u00b5g. The overall RHI score was positively correlated with CD19, CD20, CD40, FOXP3, LAG-3, PD-1 and PD-L1 concentration (\u03c1=0.596\u20130.799) and negatively correlated with CD56 concentration (\u03c1=\u22120.460). There was no significant association between RHI score and CD4, CD8, CD68, CD103, HLA-DRA or Ki67 concentration.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This study provides insight into the correlation between immune cell marker expression and histological disease activity and the possible molecular and immunological determinants underlying microscopic disease activity in UC.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/jcp-2023-209327","type":"journal-article","created":{"date-parts":[[2024,2,28]],"date-time":"2024-02-28T13:20:20Z","timestamp":1709126420000},"page":"605-613","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":7,"title":["Identification of immune cell markers associated with ulcerative colitis histological disease activity in colonic biopsies"],"prefix":"10.1136","volume":"78","author":[{"given":"Pavine L C","family":"Lefevre","sequence":"first","affiliation":[{"name":"Alimentiv Inc, London, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongya","family":"Wang","sequence":"additional","affiliation":[{"name":"Alimentiv Inc, London, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wendy","family":"Teft","sequence":"additional","affiliation":[{"name":"Alimentiv Inc, London, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangyong","family":"Zou","sequence":"additional","affiliation":[{"name":"Alimentiv Inc, London, Ontario, Canada"},{"name":"Department of Epidemiology and Biostatistics, Western University, London, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanja","family":"Van Viegen","sequence":"additional","affiliation":[{"name":"Alimentiv Inc, London, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bryan","family":"Linggi","sequence":"additional","affiliation":[{"name":"Alimentiv Inc, London, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vipul","family":"Jairath","sequence":"additional","affiliation":[{"name":"Alimentiv Inc, London, Ontario, Canada"},{"name":"Department of Epidemiology and Biostatistics, Western University, London, Ontario, Canada"},{"name":"Department of Medicine, Western University, London, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian G","family":"Feagan","sequence":"additional","affiliation":[{"name":"Alimentiv Inc, London, Ontario, Canada"},{"name":"Department of Epidemiology and Biostatistics, Western University, London, Ontario, Canada"},{"name":"Department of Medicine, Western University, London, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rish K","family":"Pai","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine & Pathology, Mayo Clinic Arizona, Scottsdale, Arizona, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niels","family":"Vande Casteele","sequence":"additional","affiliation":[{"name":"Alimentiv Inc, London, Ontario, Canada"},{"name":"Department of Medicine, University of California, La Jolla, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2024,2,28]]},"reference":[{"key":"2025121408433063000_78.9.605.1","doi-asserted-by":"publisher","DOI":"10.1097\/MOG.0000000000000082"},{"key":"2025121408433063000_78.9.605.2","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2016-312307"},{"key":"2025121408433063000_78.9.605.3","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2015-309598"},{"key":"2025121408433063000_78.9.605.4","doi-asserted-by":"publisher","DOI":"10.1038\/ajg.2016.418"},{"key":"2025121408433063000_78.9.605.5","article-title":"Histologic scoring indices for evaluation of disease activity in ulcerative colitis","volume":"5","author":"Mosli","year":"2017","journal-title":"Cochrane Database Syst Rev"},{"key":"2025121408433063000_78.9.605.6","doi-asserted-by":"publisher","DOI":"10.1136\/gut.47.3.404"},{"key":"2025121408433063000_78.9.605.7","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2015-310393"},{"key":"2025121408433063000_78.9.605.8","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1016\/j.gie.2018.08.018","article-title":"The emerging role of histologic disease activity assessment in ulcerative colitis","volume":"88","author":"Pai","year":"2018","journal-title":"Gastrointest Endosc"},{"key":"2025121408433063000_78.9.605.9","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2018-316702"},{"key":"2025121408433063000_78.9.605.10","doi-asserted-by":"crossref","first-page":"9805","DOI":"10.1038\/s41598-020-66902-0","article-title":"Analysis of immune checkpoint drug targets and tumor proteotypes in non-small cell lung cancer","volume":"10","author":"Liebler","year":"2020","journal-title":"Sci Rep"},{"key":"2025121408433063000_78.9.605.11","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa1100"},{"key":"2025121408433063000_78.9.605.12","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1093\/ecco-jcc\/jjx160","article-title":"Intestinal T cell profiling in inflammatory bowel disease: linking T cell Subsets to disease activity and disease course","volume":"12","author":"Smids","year":"2018","journal-title":"J Crohns Colitis"},{"key":"2025121408433063000_78.9.605.13","doi-asserted-by":"publisher","DOI":"10.1002\/ibd.21229"},{"key":"2025121408433063000_78.9.605.14","doi-asserted-by":"crossref","first-page":"1446","DOI":"10.1093\/ecco-jcc\/jjaa054","article-title":"Lymphocyte activation gene (LAG)-3 is associated with mucosal inflammation and disease activity in ulcerative colitis","volume":"14","author":"Slevin","year":"2020","journal-title":"J Crohns Colitis"},{"key":"2025121408433063000_78.9.605.15","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1080\/00365521.2021.1906316","article-title":"Distribution of mucosal PD-1 expressing T cells in patients with colitis of different etiologies","volume":"56","author":"Roosenboom","year":"2021","journal-title":"Scand J Gastroenterol"},{"key":"2025121408433063000_78.9.605.16","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2018.01125","article-title":"Expression of programmed death-ligand 1 by human colonic CD90(+) stromal cells differs between ulcerative colitis and crohn\u2019s disease and determines their capacity to suppress Th1 cells","volume":"9","author":"Beswick","year":"2018","journal-title":"Front Immunol"},{"key":"2025121408433063000_78.9.605.17","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2021.08.055"},{"key":"2025121408433063000_78.9.605.18","doi-asserted-by":"publisher","DOI":"10.1136\/jclinpath-2021-207718"}],"container-title":["Journal of Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jcp-2023-209327","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T16:43:40Z","timestamp":1765730620000},"score":1,"resource":{"primary":{"URL":"https:\/\/jcp.bmj.com\/lookup\/doi\/10.1136\/jcp-2023-209327"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,28]]},"references-count":18,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2025,8,18]]},"published-print":{"date-parts":[[2025,9]]}},"alternative-id":["10.1136\/jcp-2023-209327"],"URL":"https:\/\/doi.org\/10.1136\/jcp-2023-209327","relation":{},"ISSN":["0021-9746","1472-4146"],"issn-type":[{"value":"0021-9746","type":"print"},{"value":"1472-4146","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2,28]]}}}