{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T10:02:52Z","timestamp":1789725772561,"version":"4.0.1"},"reference-count":75,"publisher":"BMJ","issue":"10","funder":[{"name":"Pathology and Laboratory Medicine Academic Enrichment Fund"}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["J Clin Pathol"],"accepted":{"date-parts":[[2026,8,2]]},"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>To characterise gene expression differences between oral cavity squamous cell carcinoma (OSCC) and non-neoplastic oral mucosa using targeted transcriptome profiling and to evaluate gene expression patterns associated with tumour differentiation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Formalin-fixed, paraffin-embedded tissue from 16 resected OSCCs (3 well differentiated, 9 moderately differentiated and 4 poorly differentiated) and 7 non-neoplastic oral mucosa samples was analysed. RNA was isolated from microdissected tissue and profiled using a targeted next-generation sequencing assay. Differential gene expression and pathway enrichment analyses were performed, including comparisons across histologic grades.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>OSCC demonstrated broad transcriptomic differences compared with non-neoplastic oral mucosa, with enrichment of pathways related to extracellular matrix remodelling, cell adhesion, immune regulation, oncogenic signalling and cellular metabolism. Grade-stratified analyses identified additional differences involving epithelial differentiation, immune response, lipid metabolism and oxidative phosphorylation pathways.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>OSCC exhibits broad transcriptomic dysregulation involving extracellular matrix remodelling, cell adhesion, immune signalling, oncogenic signalling and cellular metabolism. Grade-stratified findings demonstrate molecular differences associated with tumour differentiation but require validation in larger independent cohorts. These findings may inform future studies of biomarkers and biologically relevant pathways in OSCC.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/jcp-2025-210614","type":"journal-article","created":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T17:20:16Z","timestamp":1786468816000},"page":"678-687","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":0,"title":["Targeted transcriptome profiling of oral cavity squamous cell carcinoma and histologic grade-associated gene expression patterns"],"prefix":"10.1136","volume":"79","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0498-4185","authenticated-orcid":false,"given":"Brittany","family":"Ruschkowski","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Ottawa Hospital, Ottawa, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bryan","family":"Lo","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Ottawa Hospital, Ottawa, Ontario, Canada"},{"name":"Division of Diagnostic and Molecular Pathology, The Ottawa Hospital, Ottawa, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3821-8956","authenticated-orcid":false,"given":"Jason K","family":"Wasserman","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Ottawa Hospital, Ottawa, Ontario, Canada"},{"name":"Division of Diagnostic and Molecular Pathology, The Ottawa Hospital, Ottawa, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2026,8,11]]},"reference":[{"key":"2026091802100911000_79.10.678.1","doi-asserted-by":"crossref","first-page":"3721","DOI":"10.3390\/curroncol30040283","article-title":"Oral Squamous Cell Carcinoma and Concomitant Primary Tumors, What Do We Know? 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