{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T10:09:32Z","timestamp":1779098972128,"version":"3.51.4"},"reference-count":25,"publisher":"BMJ","issue":"6","funder":[{"name":"University of Calgary, Department of Pathology and Laboratory Medicine","award":["TLPSI-RS-504"],"award-info":[{"award-number":["TLPSI-RS-504"]}]}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["J Clin Pathol"],"accepted":{"date-parts":[[2026,3,29]]},"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>To determine if the frequency of CD103+ T cells among background lymphocytes is associated with non-haematopoietic metastasis in samples of clinically concerning unexplained lymphadenopathy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We retrospectively reviewed clinical flow cytometry data from 126 lymph node (LN) samples including 36 involved by metastatic non-haematopoietic malignancy and 90 control LNs (54 benign\/reactive and 36 lymphomas). We evaluated the frequency of CD103+ cells among CD3+, CD3+CD4+ and CD3+CD8+ T\u2009cell subsets. Immunohistochemistry was used to visualise CD103 expression in a limited number of cases.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>CD103 expression in \u22653%\u2009of total CD3+ T\u2009cells and \u22657%\u2009of CD3+CD8+ T\u2009cells correlated with the presence of metastatic non-haematopoietic malignancy in LNs, and differentiated LNs with metastasis from control LNs with sensitivities of 75% (95% CI 59% to 86%) and 78% (95% CI 62% to 88%) and specificities of 100% (95% CI 96% to 100%) and 99% (95% CI 94% to 100%), respectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Increased frequency of CD103+ T\u2009cells is associated with metastatic non-haematopoietic malignancies in excisional and core needle biopsies of clinically concerning lymphadenopathy.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/jcp-2026-210641","type":"journal-article","created":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T17:20:15Z","timestamp":1775582415000},"page":"404-409","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":0,"title":["Increased CD103+ T lymphocytes in lymph nodes as a marker of metastasis from non-haematopoietic malignancies"],"prefix":"10.1136","volume":"79","author":[{"given":"Mahboobsadat","family":"Hamidi","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, University of Calgary, Calgary, Alberta, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5791-1532","authenticated-orcid":false,"given":"Jason E","family":"Love","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology, University of Washington School of Medicine, 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