{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T09:54:31Z","timestamp":1781776471190,"version":"3.54.5"},"reference-count":55,"publisher":"BMJ","issue":"7","content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["J Clin Pathol"],"accepted":{"date-parts":[[2026,3,21]]},"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Cell division cycle 25A (CDC25A) is a key regulator of cell cycle progression, DNA replication and apoptosis in cancer cells. This study employed multiple well-characterised breast cancer cohorts to evaluate the prognostic significance of CDC25A and to characterise the molecular association linked to its expression in early-stage breast cancer.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>CDC25A transcriptomic expression was systematically assessed for statistical associations with key genes and pathways implicated in cell cycle regulation, DNA damage repair, cyclin-dependent signalling, tumour microenvironment and epithelial\u2013mesenchymal transition. Its prognostic relevance was further evaluated through survival analyses. These investigations were conducted across the Molecular Taxonomy of Breast Cancer International Consortium (n=1980), the Cancer Genome Atlas (n=854) and Kaplan-Meier Plotter (n=4929) breast cancer cohorts. Subsequently, this study explored the associations between CDC25A protein expression and established clinicopathological parameters, molecular characteristics and patient outcomes using immunohistochemistry in a large, well-characterised Nottingham breast cancer cohort (n=1045).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>High CDC25A expression was associated with altered expression of key breast cancer-related genes involved in cell cycle control, DNA damage repair, cyclin-dependent signalling, matrix remodelling and epithelial\u2013mesenchymal transition-related biology. Elevated CDC25A expression at both transcriptomic and proteomic levels was significantly associated with aggressive clinicopathological features, including higher tumour grade, larger tumour size, hormone receptor negativity and lymphovascular invasion. High CDC25A protein expression independently predicted poorer survival outcomes (p=0.027; HR 1.28, 95% CI 1.18 to 1.98).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>CDC25A is an independent prognostic biomarker of clinical outcome in breast cancer. Further functional studies are warranted to validate CDC25A as a potential prognostic and therapeutic biomarker in breast cancer.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/jcp-2026-210688","type":"journal-article","created":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T17:20:33Z","timestamp":1775064033000},"page":"449-457","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":0,"title":["Insights into the prognostic significance of cell division cycle 25A (CDC25A) in breast cancer"],"prefix":"10.1136","volume":"79","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2507-2292","authenticated-orcid":false,"given":"Yousif A","family":"Kariri","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Science, Faculty of Applied Medical Science, Shaqra University, Shaqra, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mansour A","family":"Alsaleem","sequence":"additional","affiliation":[{"name":"Unit of Scientific Research, Applied College, Qassim University, Qassim, Saudi Arabia"},{"name":"Department of Clinical Research, Qassim University Medical City, Qassim University, Qassim, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bader","family":"Alshamsan","sequence":"additional","affiliation":[{"name":"Department of Medicine, College of Medicine, Qassim University, Qassim, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdulaziz","family":"Algharras","sequence":"additional","affiliation":[{"name":"Department of Radiology, College of Medicine, Qassim University, Qassim, Saudi Arabia"},{"name":"Department of Radiology, Qassim University Medical City, Qassim University, Qassim, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taher A","family":"Kariri","sequence":"additional","affiliation":[{"name":"Department of Palliative Medicine, Cancer Centre, Jazan Specialist Hospital, Jazan Health Cluster, Jazan, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5009-5525","authenticated-orcid":false,"given":"Emad A","family":"Rakha","sequence":"additional","affiliation":[{"name":"Nottingham Breast Cancer Research Centre, Academic Unit for Translational Medical Sciences, School of Medicine, University of Nottingham Biodiscovery Institute, University Park, Nottingham, UK"},{"name":"Pathology Department, Nottingham University Hospitals NHS Trust, Nottingham, UK"},{"name":"Pathology Department, Hamad Medical Corporation, Doha, Qatar"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2026,4,1]]},"reference":[{"key":"2026061802100673000_79.7.449.1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21763"},{"key":"2026061802100673000_79.7.449.2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2018.19323"},{"key":"2026061802100673000_79.7.449.3","doi-asserted-by":"publisher","DOI":"10.1111\/joim.12609"},{"key":"2026061802100673000_79.7.449.4","doi-asserted-by":"crossref","unstructured":"Ding L , Cao J , Lin W , et al . 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